The work is devoted to the study of monounsaturated fatty acids (MUFA) content in artemia enriched with biologically active substances at different stages of ontogenesis. Artemia cysts are used in the preparation of dietary supplements, highly effective feed additives for farm animals, fertilizers for crop production, chitosan for pharmaceutical industry and cosmetology, as raw materials in many other fields. When growing fish, crustaceans, shellfish in aquaculture, artemia nauplii are used as live starter feeds, which are obtained in situ when cultivating artemia cysts. Recently, the direction of enrichment of artemia nauplia used for feeding fish larvae and fry with biologically active substances has begun to develop: vitamins, essential amino acids, essential fatty acids, probiotics. In our work, we used a feed additive for the enrichment of artemia nauplia, including: vitamin-amino acid complex – «Chiktonic», probiotic – «Vetom-1», adaptogen – «Trekrezan», hemp oil. Enrichment was carried out through the skin at the stage of early nauplia. The aim of the work was to study the composition of MUFA at different stages of ontogenesis in intact artemia and artemia enriched with biologically active substances. The main function of MUFA is to activate metabolic processes and maintain homeostasis. When identifying monounsaturated fatty acids of artemia, the race we studied, the following were identified: oleic, palmitoleic, eicosenic, elaidic, erucic, nervonic, myristoleic acids. Oleic, palmitoleic and eicosenic fatty acids played a dominant role in the structure of MUFA. Two fatty acids, erucic and elaidic, can be distinguished in the composition of MUFA, the content of which was minimal.