Individual behavior related to oral health could cause poor oral condition. Health behavior consists of domains of knowledge, attitude, and action related to health, including oral health. This study was aimed to evaluate the relationship between knowledge and action to maintain oral health and subjective complaints related to oral condition of medical and non-medical students. This was an analytical and observational study with a cross sectional design. Samples were medical and non-medical students obtained by using the consecutive sampling technique. Data were collected at Diponegoro University using online questionnaire through the Google form platform regarding knowledge, actions to maintain oral health, and subjective complaints of oral health problems. Data were analyzed by using the chi-square test. The results showed that the majority of students had a high level of knowledge about dental and oral health, meanwhile, their action to maintain oral health was in moderate level. The levels of knowledge and action to maintain oral health of medical students were better than of the non-medical students. Dental and oral health problems most frequently complained were recurrent aphtous ulcers (RAU) and dental caries. The chi-square test showed a p-value of 0.023 between the action to maintain oral health and subjective complaints of oral health problems. In conclusion, there was a significant relationship between the action to maintain oral health and subjective complaints of oral health problems.Keywords: knowledge, action to maintain oral health, dental and oral health problem Abstrak: Salah satu faktor penyebab permasalahan gigi dan mulut ialah perilaku terkait kesehatan gigi dan mulut. Perilaku kesehatan terdiri dari domain pengetahuan, sikap, dan tindakan yang berkait-an dengan kesehatan termasuk kesehatan gigi dan mulut. Penelitian ini bertujuan untuk mengetahui hubungan pengetahuan dan tindakan menjaga kesehatan gigi dan mulut dengan keluhan subyektif permasalahan gigi dan mulut pada mahasiswa kesehatan dan non kesehatan. Jenis penelitian ialah observasional analitik dengan desain potong lintang. Sampel penelitian ialah mahasiswa kesehatan dan non kesehatan, diperoleh dengan teknik consecutive sampling. Pengambilan data dilakukan di Universitas Diponegoro dengan menggunakan kuesioner online melalui platform Google form ten-tang pengetahuan, tindakan menjaga kesehatan gigi dan mulut, dan keluhan subyektif permasalahan kesehatan gigi dan mulut. Analisis statistik menggunakan uji chi-square. Hasil penelitian menun-jukkan tingkat pengetahuan kesehatan gigi dan mulut mahasiswa umumnya tinggi, sedangkan tindak-an menjaga kesehatan gigi dan mulut umumnya berada pada kategori sedang. Tingkat pengetahuan dan tindakan menjaga kesehatan gigi dan mulut mahasiswa kesehatan lebih baik daripada yang non kesehatan. Permasalahan yang terbanyak dikeluhkan ialah recurrent aphtous ulcers (RAU) dan karies gigi. Uji chi-square mendapatkan nilai p=0,023 untuk hubungan tindakan menjaga kesehatan gigi dan mulut dengan keluhan subyektif permasalahan kesehatan gigi dan mulut. Simpulan penelitian ini ialah terdapat hubungan bermakna antara tindakan menjaga kesehatan gigi dan mulut dengan keluhan subyektif permasalahan kesehatan gigi dan mulut.Kata kunci: pengetahuan, tindakan menjaga kesehatan gigi dan mulut, masalah kesehatan gigi mulut