Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Velazquez, Fabiola N.; Miretti, Mariana; Baumgartner, María T.; Caputto, Beatriz L.; Tempesti, Tomás Cristian; Prucca, César G.

doi:10.1038/s41598-019-39390-0

Cited by 25 publications

(9 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It could be thought that the irradiation time employed in the present photoinactivation experiments may not be so practical. However, let us note that such large times are not unusual as reported in other photoinactivation studies [52][53][54][55][56].…”

Section: Plos Onesupporting

confidence: 73%

The growth of Escherichia coli cultures under the influence of pheomelanin nanoparticles and a chelant agent in the presence of light

et al. 2022

View full text Add to dashboard Cite

Growing concern of antibiotic resistance has increased research efforts to find nonspecific treatments to inhibit pathogenic microorganisms. In this regard, photodynamic inactivation is a promising method. It is based on the excitation of a photosensitizer molecule (PS) with UV-Vis radiation to produce reactive oxygen species. The high reactivity of such species nearby the PS leads to oxidation of bacterial cell walls, lipid membranes (lipid peroxidation), enzymes, and nucleic acids, eventually producing cell death. In the last decade, many studies have been carried out with different photosensitizers to suppress the growth of bacteria, fungi, viruses, and malignant tumors. Here, our main motivation is to employ pheomelanin nanoparticles as sensitizers for inhibiting the growth of the Gram-negative bacteria E. coli, exposed to blue and UVA radiation. In order to perform our experiments, we synthesized pheomelanin nanoparticles from L-DOPA and L-cysteine through an oxidation process. We carried out experiments at different particle concentrations and different energy fluences. We found that cultures exposed to UVA at 166 μg/mL and 270 J/cm2, in conjunction with ethylenediaminetetraacetic acid (EDTA) as an enhancer, decreased in the viable count 5 log10. Different reactive oxygen species (singlet oxygen, hydroxyl radicals, and peroxynitrates) were detected using different procedures. Our results suggest that the method reported here is effective against E. coli, which could encourage further investigations in other type of bacteria.

show abstract

Section: Plos Onesupporting

confidence: 73%

The growth of Escherichia coli cultures under the influence of pheomelanin nanoparticles and a chelant agent in the presence of light

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The differences in sensitivity to each of the compounds observed between particular cell lines most likely result from the aforementioned biodiversity of cell lines used in this study. Indeed, several groups have documented a differential response of those three cell lines to various therapeutic approaches [ 55 , 56 , 57 , 58 ]. Here, compared to T98G and LN229 cells, U87MG cells turned out to be less susceptible to dendrimer-mediated toxicity, which was particularly pronounced in colony-forming assay.…”

Section: Discussionmentioning

confidence: 99%

Design, Synthesis and Activity of New N1-Alkyl Tryptophan Functionalized Dendrimeric Peptides against Glioblastoma

et al. 2022

View full text Add to dashboard Cite

Background: Due to resistance to conventional therapy, a blood–brain barrier that results in poor drug delivery, and a high potential for metastasis, glioblastoma (GBM) presents a great medical challenge. Since the repertoire of the possible therapies is very limited, novel therapeutic strategies require new drugs as well as new approaches. The multiple roles played by L-tryptophan (Trp) in tumorigenesis of GBM and the previously found antiproliferative properties of Trp-bearing dendrimers against this malignancy prompted us to design novel polyfunctional peptide-based dendrimers covalently attached to N1-alkyl tryptophan (Trp) residues. Their antiproliferative properties against GBM and normal human astrocytes (NHA) and their antioxidant potential were tested. Methods: Two groups of amphiphilic peptide dendrimers terminated with N1-butyl and N1-aminopentane tryptophan were designed. The influence of dendrimers on viability of NHA and human GBM cell lines, displaying different genetic backgrounds and tumorigenic potentials, was determined by the MTT test. The influence of compounds on the clonogenic potential of GBM cells was assessed by colony-formation assay. Dendrimers were tested for radical scavenging potency as well as redox capability (DPPH, ABTS, and FRAP models). Results: Several peptide dendrimers functionalized with N1-alkyl-tryptophan at 5 µM concentration exhibited high selectivity towards GBM cells retaining 85–95% viable NHA cells while killing cancer cells. In both the MTT and colony-formation assays, compounds 21 (functionalized with N1-butyl-Trp and (+)8 charged) and 25 (functionalized with N1-aminopentane-Trp and (+)12 charged) showed the most promise for their development into anticancer drugs. According to ABTS, DPPH, and FRAP antioxidant tests, dendrimers functionalized with N1-alkylated Trp expressed higher ROS-scavenging capacity (ABTS and DPPH) than those with unsubstituted Trp. Conclusions: Peptide dendrimers functionalized with N1-alkyl-tryptophan showed varying toxicity to NHA, while all were toxic to GBM cells. Based on their activity towards inhibition of GBM viability and relatively mild effect on NHA cells the most advantageous were derivatives 21 and 25 with the respective di-dodecyl and dodecyl residue located at the C-terminus. As expected, peptide dendrimers functionalized with N1-alkyl-tryptophan expressed higher scavenging potency against ROS than dendrimers with unsubstituted tryptophan.

show abstract

“…Зокрема in vitro показано протипухлинний ефект ФДТ з 5-ALA у культурах клітин ЗГ SU-DIPG-XIII у поєднанні з фракціонованою радіотерапією [56]. Фталоціаніни ZnPc і TAZnPc in vitro спричиняли загибель клітин ГБ ліній T98G, MO59, LN229 та U87-MG шляхом апоптозу, що супроводжувалось активацією каспази 3, втратою морфології і функцій мітохондрій, цілісності лізосом, екс терналізацією фосфатидилсерину та фрагментацією ДНК пух линних к літин [61]. Виявлено протипухлинні ефекти поєднання ФДТ синопорфірином натрію і сонодинамічної терапії in vitro у клітинах гліоми U118 [57].…”

Section: експериментальні дослідження фотодинамічної терапіїunclassified

Photodynamic therapy of malignant brain gliomas

Rozumenko¹,

Любич²,

Стайно³

et al. 2022

Ukr Neurosurg J

View full text Add to dashboard Cite

Treatment of malignant gliomas of the brain remains a serious problem on a global scale, despite intensive research into the causes and mechanisms of their progression. When using traditional surgical approaches and imaging methods tumor cell infiltrates may be overlooked, as a result of which, malignant gliomas relapse often occurs near the marginal region of the surgical cavity. A method that allows visual identification of tumor tissue and at the same time provides an opportunity to selectively destroy it is photodynamic therapy (PDT) ‒ a two-stage treatment that includes the introduction (intravenous, intraperitoneal, local or oral) of a light-sensitive chemical agent (photosensitizer (PS)) followed by its activation at a certain wavelength of light.The principle of PDT is based on the cytotoxic effects caused by PS, which selectively accumulates in malignant tumor cells and is activated by light rays of the appropriate wavelength, generating singlet oxygen and free radicals, which trigger photochemical reactions in tumor cells with subsequent destruction of protein structures. Tumor tissue has a higher affinity for PSs. PSs are divided into 1st, 2nd and 3rd generation molecules. So far, 3rd generation PSs have not yet been approved for clinical use. In vitro and in vivo experimental studies confirmed the effectiveness of PDT of brain tumors using 2nd generation PSs.The simultaneous use of surgery under the control of fluorescence and PDT enables both the visualization of tumor cells and their selective destruction. Regardless of PDT, PSs are used for the purpose of auxiliary delineation of tumor borders for maximum tumor removal during fluorescence-guided surgery.The review examines the development of PDT in a historical aspect, the contribution of domestic scientists, in particular, scientists of the Institute of Neurosurgery named after acad. A. P. Romodanov, National Academy of Medical Sciences of Ukraine to the development of the problem of PDT in neuro-oncology; preclinical studies of PDT and experimental approaches to increase the efficiency of PDT are characterized. Analysis of data from clinical trials confirms that using PDT as an adjunctive treatment of malignant gliomas administered immediately after maximal resection is safe, reduces the risk of recurrence by targeting residual tumor cells in the resection cavity, improves survival and quality of life of patients. The absence of information on the development of resistance to multiple PDT sessions suggests the possibility of repeated treatments of tumor cells not removed during surgery.

show abstract

Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Cited by 25 publications

References 64 publications

The growth of Escherichia coli cultures under the influence of pheomelanin nanoparticles and a chelant agent in the presence of light

The growth of Escherichia coli cultures under the influence of pheomelanin nanoparticles and a chelant agent in the presence of light

Design, Synthesis and Activity of New N1-Alkyl Tryptophan Functionalized Dendrimeric Peptides against Glioblastoma

Photodynamic therapy of malignant brain gliomas

Contact Info

Product

Resources

About