Effectiveness of γ-oryzanol in reducing neuromotor deficits, dopamine depletion and oxidative stress in a Drosophila melanogaster model of Parkinson's disease induced by rotenone

Araújo, Stífani Machado; Paula, Mariane Trindade de; Poetini, Márcia Rósula; Meichtry, Luana Barreto; Bortolotto, Vandreza Cardoso; Zarzecki, Micheli Stéfani; Jessé, Cristiano Ricardo; Prigol, Marina

doi:10.1016/j.neuro.2015.09.003

Cited by 48 publications

(17 citation statements)

References 58 publications

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“…The production of free radicals and oxidative stress are among the deleterious factors associated with neuronal mitochondrial dysfunction. In a Drosophila melanogaster model of PD induced by rotenone, Orz improved antioxidant defenses, prevented oxidative stress, and attenuated mitochondrial dysfunction [33]. A significant increase in antioxidant enzymes (such as catalase, superoxide dismutase, and glutathione-S-transferase) was also observed and linked to abrogation of deleterious MDA and ROS produced by rotatone [33].…”

Section: Antioxidant Activity Of γ-Oryzanolmentioning

confidence: 99%

“…In a Drosophila melanogaster model of PD induced by rotenone, Orz improved antioxidant defenses, prevented oxidative stress, and attenuated mitochondrial dysfunction [33]. A significant increase in antioxidant enzymes (such as catalase, superoxide dismutase, and glutathione-S-transferase) was also observed and linked to abrogation of deleterious MDA and ROS produced by rotatone [33]. These activities are likely associated to the inhibition of free radical generation and the consequent prevention of inflammation progress.…”

Section: Antioxidant Activity Of γ-Oryzanolmentioning

confidence: 99%

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Antioxidant Activity of γ-Oryzanol: A Complex Network of Interactions

Minatel

Francisqueti

Corrêa

et al. 2016

IJMS

126

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γ-oryzanol (Orz), a steryl ferulate extracted from rice bran layer, exerts a wide spectrum of biological activities. In addition to its antioxidant activity, Orz is often associated with cholesterol-lowering, anti-inflammatory, anti-cancer and anti-diabetic effects. In recent years, the usefulness of Orz has been studied for the treatment of metabolic diseases, as it acts to ameliorate insulin activity, cholesterol metabolism, and associated chronic inflammation. Previous studies have shown the direct action of Orz when downregulating the expression of genes that encode proteins related to adiposity (CCAAT/enhancer binding proteins (C/EBPs)), inflammatory responses (nuclear factor kappa-B (NF-κB)), and metabolic syndrome (peroxisome proliferator-activated receptors (PPARs)). It is likely that this wide range of beneficial activities results from a complex network of interactions and signals triggered, and/or inhibited by its antioxidant properties. This review focuses on the significance of Orz in metabolic disorders, which feature remarkable oxidative imbalance, such as impaired glucose metabolism, obesity, and inflammation.

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Section: Antioxidant Activity Of γ-Oryzanolmentioning

confidence: 99%

Section: Antioxidant Activity Of γ-Oryzanolmentioning

confidence: 99%

Antioxidant Activity of γ-Oryzanol: A Complex Network of Interactions

Minatel

Francisqueti

Corrêa

et al. 2016

IJMS

126

View full text Add to dashboard Cite

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“…It has been extensively used as an antioxidant, antiulcerogenic, antineoplastic, antidiabetic, and antiallergic drug for many years (Islam et al, 2011;Kim et al, 2012;Kozuka et al, 2013;Goufo and Trindade, 2014;Kozuka et al, 2015). Therapeutic use of GORZ has also been approved for inflammation and nervousness (Chotimarkorn and Ushio, 2008;Araujo et al, 2015). In addition, GORZ can decrease the risk of HFDinduced obesity by inhibiting HFD-induced hyperlipidemia and oxidative stress in mice (Jin Son et al, 2010), although very little is known about the molecular mechanisms involved in this process.…”

Section: Introductionmentioning

confidence: 99%

Gamma Oryzanol Alleviates High-Fat Diet-Induced Anxiety-Like Behaviors Through Downregulation of Dopamine and Inflammation in the Amygdala of Mice

et al. 2020

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Background: A high-fat diet (HFD) can induce obesity and metabolic disorders that are closely associated with cognitive impairments, and the progression of several psychiatric disorders such as anxiety. We have previously demonstrated the anxiolytic-like effect of Gamma oryzanol (GORZ) in chronic restraint stressed mice.Objective: We studied the neurochemical and molecular mechanisms that underlie the preventive effect of GORZ in HFD-induced anxiety-like behaviors, monoaminergic dysfunction, and inflammation.Methods: Eight-week-old Institute of Cancer (ICR) male mice weighing 33-34 g were divided into the following groups and free-fed for 8 weeks: control (14% casein, AIN 93M); HFD; HFD + GORZ (0.5% GORZ). Body weight gain was checked weekly. The anxiolyticlike effects of GORZ were examined via open-field test (OFT) and elevated plus maze (EPM) test. Brain levels of monoamines [5-hydroxy tryptamine (5-HT), dopamine (DA), and norepinephrine (NE)] and their metabolites [5-hydroxyindole acetic acid (5-HIAA), homovanillic acid (HVA), and 3-methoxy-4-hydroxyphenylglycol (MHPG)], proinflammatory cytokines such as tumor necrosis factor-aa (Tnf-a) mRNA levels, and interleukin 1-b (Il-1b) mRNA levels in the cerebral cortex and amygdala were examined using high-performance liquid chromatography-electrochemical detection (HPLC-ECD), and real-time reverse transcription-polymerase chain reaction (RT-PCR), respectively.Results: Mice fed a HFD for eight weeks showed anxiety-like behaviors in association with HFD-induced body weight gain. GORZ potentially blocked HFD-induced anxiety-like behaviors via significant improvement of the primary behavioral parameters in behavioral tests, with a minor reduction in HFD-induced body weight gain. Furthermore, GORZ treatment significantly downregulated HFD-induced upregulation of dopamine levels in the brain's amygdala. Significant reduction of the relative mRNA expression of Tnf-a and Il-1 b was also observed in the amygdala of HFD + GORZ mice, compared to HFD mice. Conclusions:Our findings strongly suggest that 0.5% GORZ exerts anxiolytic-like effects, possibly through downregulation of dopamine, and via expression of proinflammatory cytokines Tnf-a and Il-1 b in the case of chronic HFD exposure.

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“…22 An alternative biological model to test the beta-carotene effects is Drosophila melanogaster (DM), which is widely used in biological tests due to its great similarity to the human genetic sequence, allowing the understanding of complex biological problems. 19,23 Toxicity of beta-carotene has been investigated due to the use of beta-carotene as a dietary supplement. As stated by Sacha and co-workers, 24 beta-carotene is able to inhibit the cell growth of tumor cells like lung, melanoma, colon and breast cancer.…”

Section: Introductionmentioning

confidence: 99%

Nanodispersions of beta-carotene: effects on antioxidant enzymes and cytotoxic properties

et al. 2018

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Beta-carotene is a carotenoid precursor of vitamin A, known for its biological activities. Due to its high hydrophobicity, nanonization processes, i.e. the transformation into nanoparticles, can improve its water affinity, and therefore the activity in aqueous systems. The objective of this study was to produce beta-carotene nanoparticles by the solid dispersion method and to evaluate their effects on the activity of glutathione-S-transferase and acetylcholinesterase enzymes using Drosophila melanogaster (DM) homogenate, the superoxide dismutase- and catalase-like activities under in vitro conditions, and their cytotoxic properties against tumor and non-tumor cells. The formed nanometric beta-carotene particles resulted in stable colloids, readily dispersed in water, able to modulate acetylcholinesterase (AChE) activity, and presenting high potential to control the cholinergic system. Beta-carotene nanoparticles, at concentrations much lower than the pure pristine beta-carotene, presented in vitro mimetic activity to superoxide dismutase and altered glutathione-S-transferase activity in DM tissue. The content of hydrogen peroxide was neither affected by the nanoparticles (in aqueous solution) nor by pristine beta-carotene (in DMSO). In the cytotoxic assays, beta-carotene nanoparticles dispersed in water showed activity against four different tumor cell lines. Overall, beta-carotene nanoparticles presented significant bioactivity in aqueous medium surpassing their high hydrophobicity constraint.

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Effectiveness of γ-oryzanol in reducing neuromotor deficits, dopamine depletion and oxidative stress in a Drosophila melanogaster model of Parkinson's disease induced by rotenone

Cited by 48 publications

References 58 publications

Antioxidant Activity of γ-Oryzanol: A Complex Network of Interactions

Antioxidant Activity of γ-Oryzanol: A Complex Network of Interactions

Gamma Oryzanol Alleviates High-Fat Diet-Induced Anxiety-Like Behaviors Through Downregulation of Dopamine and Inflammation in the Amygdala of Mice

Nanodispersions of beta-carotene: effects on antioxidant enzymes and cytotoxic properties

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