Effects 4-(1-adamantyl)-phenoxy-3-(n-benzyl, n-dimethylamino)-2-propanol chloride on the strains of Pseudomonas Spp.

Dudikova, D. M.; Voychuk, S.I.; Vrynchanu, N. O.

doi:10.15587/2519-8025.2018.141396

ScienceRise: Biological Science

2018

DOI: 10.15587/2519-8025.2018.141396

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Effects 4-(1-adamantyl)-phenoxy-3-(n-benzyl, n-dimethylamino)-2-propanol chloride on the strains of Pseudomonas Spp.

D. M. Dudikova¹,

S.I. Voychuk²,

N. O. Vrynchanu³

Abstract: Одним из основных возбудителей внутрибольничных инфекций является Pseudomonas aeruginosa, высокая устойчивость которого к современным антимикробным средствам приводит к снижению эффективности антибактериальной химиотерапии и необходимости поиска новых активных соединений. Производные адамантана, проявляющие широкий спектр биологической активности, могут быть рассмотрены как перспективный класс веществ с антимикробным действием. Цель. Установить чувствительность и изменения ультраструктуры P. aeruginosa при дей… Show more

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2022

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“…Th is eff ect is possibly associated with changes in the adhesion and formation of a monolayer. It completed recently found data that adamantane-containing substances have a membranotropic eff ect, inhibit effl ux pumps of microorganisms, disrupt respiration, and bind to proteins and DNA [13,14,29,30].…”

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confidence: 99%

Antibiofilm Activity of 4-(Adamantyl-1)-1-(1-Aminobutyl) Benzol against Methicillin-Resistant Staphylococcus aureus

Hrynchuk¹,

Zelena²,

Bukhtiarova³

et al. 2022

Mikrobiol. Z.

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Staphylococcus aureus is a widespread opportunistic pathogen, causing community-acquired and nosocomial infections with both acute and chronic recurrent courses. The process of chronicity of the disease is provided by biofilms. Features of the structure and functioning of biofilms, in particular the presence of matrix, quorum sensing systems, persistent cells, and efflux pumps, provide microbial communities with resistance to antimicrobial drugs under their action in therapeutic concentrations. The insufficient eff ectiveness of modern antimicrobial chemotherapy against biofi lm microorganisms indicates the urgency of the problem to search for compounds with antibiofilm activity that can affect various stages of the biofilm formation and the formed biofilm. The aim of the study is to establish the antibiofilm activity of 4-(adamantyl-1)-1-(1-aminobutyl) benzol against methicillin-resistant S. aureus (MRSA) and to determine the mechanism of its action. Methods. The ability of adamantane-containing compound 4-(adamantyl-1)-1-(1-aminobutyl) benzol (AM-166) to prevent biofilm formation and destroy the formed biofilm of S. aureus was investigated on polystyrene plates by the sorption of gentian violet on its structures followed with desorption of the dye into the organic solvent. The viability of S. aureus cells at the first stage of biofilm formation and in the composition of mature biofilms was evaluated using specific dyes for living (acridine orange) and non-viable (propidium iodide) cells. Detection of genes responsible for antibiotic resistance and biofi lm formation was performed by the polymerase chain reaction (PCR) with detection of PCR products in agarose gel. Evaluation of the effect of AM-166 on the expression of genes regulating the biofilm formation (ica, agrA, sarA, and sigB) was investigated by the real-time PCR and semi-quantitative PCR. Results. It was found that the compound AM-166 shows activity against S. aureus biofilm formation. The most pronounced effect was registered at a concentration of 5.0 minimum inhibitory concentration (MIC) (92.3%.) Under the action of AM-166 on the formed 2-day biofilms, their destruction was marked: the biomass decreases by 30.9% at 5.0 MIC. According to the results of fluorescence microscopy, the adamantane derivative at 5.0 MIC helps to reduce the number of viable cells at different stages of formation of the S. aureus biofilm. The results of molecular genetic studies indicate that the ica gene expression is significantly inhibited by the action of subinhibitory concentrations of the compound AM-116. No significant changes in the expression of sarA, agrA, and sigB genes were registered. Conclusions. Experiments on the effect of adamantane derivative on S. aureus biofilms showed that the most pronounced activity of AM-116 was observed at the stage of biofilm formation, as evidenced by the inhibition of transcriptional activity of the ica gene responsible for early stages of the biofilm formation, in particular the adhesion of planktonic cells to the substrate.

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mentioning

confidence: 99%

Antibiofilm Activity of 4-(Adamantyl-1)-1-(1-Aminobutyl) Benzol against Methicillin-Resistant Staphylococcus aureus

Hrynchuk¹,

Zelena²,

Bukhtiarova³

et al. 2022

Mikrobiol. Z.

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Effects 4-(1-adamantyl)-phenoxy-3-(n-benzyl, n-dimethylamino)-2-propanol chloride on the strains of Pseudomonas Spp.

Cited by 1 publication

References 6 publications

Antibiofilm Activity of 4-(Adamantyl-1)-1-(1-Aminobutyl) Benzol against Methicillin-Resistant Staphylococcus aureus

Antibiofilm Activity of 4-(Adamantyl-1)-1-(1-Aminobutyl) Benzol against Methicillin-Resistant Staphylococcus aureus

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