Effects and detection of Nandrosol and ractopamine administration in veal calves

Divari, Sara; Berio, Enrica; Pregel, Paola; Sereno, Alessandra; Chiesa, Luca Maria; Pavlović, Radmila; Panseri, Sara; Bovee, Toine F.H.; Biolatti, B.; Cannizzo, Francesca Tiziana

doi:10.1016/j.foodchem.2016.11.116

Cited by 8 publications

(3 citation statements)

References 43 publications

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“…7,8 So far, some different analytical methods have been developed to detect ractopamine. These methods mainly include high-performance liquid chromatography (HPLC), 9 gas and liquid chromatography (GC and LC, respectively) GC−MS, 10 LC−MS, 11 capillary electrophoresis, 12 and immunoassay. 13 Compared with the above analysis methods, the electrochemical detection of ractopamine has the advantages of low cost, less time-consuming, simple instrument operation, high sensitivity, and so on.…”

Section: Introductionmentioning

confidence: 99%

Biochar-Supported Cu²⁺/Cu⁺ Composite as an Electrochemical Ultrasensitive Interface for Ractopamine Detection

Cao

Ding

Liu

et al. 2021

ACS Appl. Bio Mater.

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Ractopamine as an important β-agonist is frequently found in pork to enhance food quality, which is harmful to human health. Thus, it is extremely important to develop an efficient analytical method to detect ractopamine for food safety control. Herein, we develop a kind of electrochemical biosensor using a biochar-supported Cu2+/Cu+ composite as an electrochemical sensing interface for detecting ractopamine. The electrochemical sensor combines the collective advantages of Nafion (enriches ractopamine and effectively shields the interference of negatively charged compounds), biochar (unique three-dimensional porous network structure to increase the contact area), and Cu2+/Cu+ (excellent electrical conductivity to speed up the charge transfer rate), which are beneficial to the accumulation and electrochemical sensing of targets on a Nafion-biochar-supported Cu2+/Cu+ electrode (NBC-GCE). The as-prepared sensor shows a good electrochemical sensing ability, with an ultralow detection limit and high sensitivity (0.041 μM and 416 μA·mM–1·cm–2, respectively), in the 0.1–1.75 μM range. In addition, the stability, repeatability, and anti-interference performance of the modified electrode are also satisfying. Last, its practicability is shown for assaying ractopamine in pork sausages. This research provides an environmentally friendly method to simultaneously treat food waste and achieve ultrasensitive detection of ractopamine in food.

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Section: Introductionmentioning

confidence: 99%

Biochar-Supported Cu²⁺/Cu⁺ Composite as an Electrochemical Ultrasensitive Interface for Ractopamine Detection

Cao

Ding

Liu

et al. 2021

ACS Appl. Bio Mater.

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“…Commonly SARMs are detected by chromatographic methods often linked with mass spectrometric detector systems, from a variety of media including hair, urine, 9,10 nails 11 and serum. 12 In all these, low or sub nM detection (pg or ng per mL mg −1 ) is observed.…”

Section: Introductionmentioning

confidence: 99%

Detection of selective androgen receptor modulators (SARMs) in serum using a molecularly imprinted nanoparticle surface plasmon resonance sensor

et al. 2022

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“…According to Dunshea et al (2005) they are absorbed into the blood and bind to these specific β-receptors on the muscle cell membrane and the primary response is to increase the muscle fibre size. They act as repartitioning agents to modify carcass composition by altering nutrient partitioning to increase muscle protein content up to 40% and lower fat deposition up to 40% (Herago and Agonafir 2017) due the inhibition of proteolysis and enhanced lipolysis, respectively (Divari et al 2017).…”

Section: Ractopamine Properties Carcass Improvement and Meat Qualitymentioning

confidence: 99%

Ractopamine: analytical method validation; and the detection in loin, tissues and urine of pigs fed meat and bone meal containing this growth promoter

Aroeira¹

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Ractopamine: analytical method validation; and the detection in loin, tissues and urine of pigs fed meat and bone meal containing this growth promoter Ractopamine hydrochloride (RAC) is a β-agonist additive that has been used in many countries as a repartitioning agent, redirecting nutrients in order to increase leanness and decrease lipid deposition in pigs. Countries from the European Union and Asia question their safety, while American countries and Australia allow their controlled use as an additive added to the feed of pigsin the finishing phase. In Brazil, the Ministry of Agriculture, Livestock and Food Supply together with the national production sector, developed a Program called "SplitSystem" to ensure a safe product without RAC in order to meet international sanitary requirements. However, coproducts used in animal feed may contain RAC, such as meat and bone meal (MBM), one of the main feed ingredients used in many countries which can partially replace soybean meal to lower costs. As the level of RAC in this protein source has not been established an experiment was under taken to examine the impact on pig tissues of increasing amounts of meat and bone meal (MBM) in four dietary groups: 0, 7, 14 and 21% w/w of MBM-containing RAC (53.5 µg kg-1) in the diet. The purpose was to verify if ractopamine residues remain in pig tissues (muscle, liver, kidneys, and lungs) and how much is eliminated through urine. To addrress these concerns, gilts were fed RAC via MBM daily, from weaning until slaughter. RAC was determined in muscle, liver, kidneys, and lungs with a limit of detection (LOD) = 0.15, 0.5, 0.5 and 1.0 µg kg-1 , respectively), and no RAC residues were quantified above the limit of quantification (LOQ) = 0.5, 2.5, 2.5 and 2.5 µg kg-1 , respectively). In urine, RAC concentration remained below 1.35 µg L-1. These values are below the maximum residue limits (MRLs) established by legislation. Therefore, MBM (53.5 μg kg-1 of RAC) can be used up to 21% in pig diets, however when considering restrictive markets, it is recommended not to use MBM.

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Effects and detection of Nandrosol and ractopamine administration in veal calves

Cited by 8 publications

References 43 publications

Biochar-Supported Cu²⁺/Cu⁺ Composite as an Electrochemical Ultrasensitive Interface for Ractopamine Detection

Biochar-Supported Cu²⁺/Cu⁺ Composite as an Electrochemical Ultrasensitive Interface for Ractopamine Detection

Detection of selective androgen receptor modulators (SARMs) in serum using a molecularly imprinted nanoparticle surface plasmon resonance sensor

Ractopamine: analytical method validation; and the detection in loin, tissues and urine of pigs fed meat and bone meal containing this growth promoter

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Effects and detection of Nandrosol and ractopamine administration in veal calves

Cited by 8 publications

References 43 publications

Biochar-Supported Cu2+/Cu+ Composite as an Electrochemical Ultrasensitive Interface for Ractopamine Detection

Biochar-Supported Cu2+/Cu+ Composite as an Electrochemical Ultrasensitive Interface for Ractopamine Detection

Detection of selective androgen receptor modulators (SARMs) in serum using a molecularly imprinted nanoparticle surface plasmon resonance sensor

Ractopamine: analytical method validation; and the detection in loin, tissues and urine of pigs fed meat and bone meal containing this growth promoter

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Product

Resources

About

Biochar-Supported Cu²⁺/Cu⁺ Composite as an Electrochemical Ultrasensitive Interface for Ractopamine Detection

Biochar-Supported Cu²⁺/Cu⁺ Composite as an Electrochemical Ultrasensitive Interface for Ractopamine Detection