Effects and Mechanism of Metal Ions on Enzymatic Hydrolysis of Wheat Straw after Pretreatment

Wang, Shan; Lv, Mengdie; Yang, Junhong; Zhou, Yongfeng; Xu, Bin

doi:10.15376/biores.13.2.2617-2631

Cited by 15 publications

(21 citation statements)

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“…Metals salts are especially fascinating as pretreatment agents because of their lower corrosivity than inorganic acids [22]. Typical and common metal salts, such as chlorides, nitrates, and sulfates of Al, Fe, Mg, and K, are usually chosen for the pretreatment of lignocellulosic biomass [23, 24]. Because of the poor contact between metal salts and cellulosic materials, metal salt pretreatment cannot be performed in solid-phase condition but in molten state or aqueous solution [25].…”

Section: Introductionmentioning

confidence: 99%

Overcoming biomass recalcitrance by synergistic pretreatment of mechanical activation and metal salt for enhancing enzymatic conversion of lignocellulose

Zhang

Huang

et al. 2019

Biotechnol Biofuels

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BackgroundDue to biomass recalcitrance, including complexity of lignocellulosic matrix, crystallinity of cellulose, and inhibition of lignin, the bioconversion of lignocellulosic biomass is difficult and inefficient. The aim of this study is to investigate an effective and green pretreatment method for overcoming biomass recalcitrance of lignocellulose.ResultsAn effective mechanical activation (MA) + metal salt (MAMS) technology was applied to pretreat sugarcane bagasse (SCB), a typical kind of lignocellulosic biomass, in a stirring ball mill. Chlorides and nitrates of Al and Fe showed better synergistic effect with MA, especially AlCl3, ascribing to the interaction between metal salt and oxygen-containing groups induced by MA. Comparative studies showed that MAMS pretreatment effectively changed the recalcitrant structural characteristics of lignocellulosic matrix and reduced the inhibitory action of lignin on enzymatic conversion of SCB. The increase in hydroxyl and carboxyl groups of lignin induced by MAMS pretreatment led to the increase of its hydrophilicity, which could weaken the binding force between cellulase and lignin and reduce the nonproductive binding of cellulase enzymes to lignin.ConclusionsMAMS pretreatment significantly enhanced the enzymatic digestibility of polysaccharides substrate by overcoming biomass recalcitrance without the removal of lignin from enzymatic hydrolysis system.Electronic supplementary materialThe online version of this article (10.1186/s13068-019-1354-6) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Overcoming biomass recalcitrance by synergistic pretreatment of mechanical activation and metal salt for enhancing enzymatic conversion of lignocellulose

Zhang

Huang

et al. 2019

Biotechnol Biofuels

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“…Metals salts are especially fascinating as pretreatment agents due to their lower corrosiveness than inorganic acids (Xu et al 2018). The common metal salts, such as the chlorides, nitrates, and sulfates of Al, Fe, Mg, and K, are typically selected for this pretreatment (Sewsynker-Sukai and Guegim Kana 2017; Wang et al 2018b). Typically, the metal salt pretreatment is carried out in the molten state or in an aqueous solution due to the poor contact between metal salts and cellulosic materials in the solid-phase condition (Loow et al 2015).…”

Section: Physical-chemical Methodsmentioning

confidence: 99%

Toward sustainable processes of pretreatment technologies of lignocellulosic biomass for enzymatic production of biofuels and chemicals: A review

Ethaib

Omar

Kamal

et al. 2020

BioRes

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Lignocellulosic biomass is a class of sustainable material that can be utilized as a raw feedstock in biofuel and chemical production. However, the complex matrix structure of lignocellulosic materials complicates conversion processes, such as enzymatic hydrolysis. Therefore, an efficient pretreatment process is required to disrupt the plant cell wall structure and maximize the recovery of valuable soluble components from lignocellulosic biomass during hydrolysis. In addition, an effective pretreatment method should use the minimum necessary amounts of energy and chemicals to minimize the cost of the end product. Further, it should reduce the formation of inhibitory compounds that affect enzymes and microorganisms during hydrolysis and fermentation, and it should be applicable to a wide variety of feedstocks. The research presented in this review has highlighted the pros and cons of the current technologies employed in pretreatment processes. Further study should be done to optimize and improve these technologies to enhance the efficiency of the production of biofuels and other valuable components.

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“…At higher concentrations, metals ions might have bound to the active site of protease K and resulted in the significantly reduced catalytic activity [28][29][30]. In addition, metal ions such as Fe(III) and Al(III) may diminish the affinity between the substrate of collagen fiber and proteinase K [31].…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Screening of additives to reduce grain damage risk on unhairing by proteinase K

Chen

Jiang

et al. 2020

J Leather Sci Eng

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Enzymatic unhairing is a cleaner strategy for leather-making. It is a potential alternative to the traditional hair-burning process. However, several shortcomings, such as uncontrolled enzymatic reaction, and risk of grain looseness and damage have restricted the broad application of enzymatic unhairing. In this work, metal ions and organic additives were screened for lessening the hydrolytic activity of proteinase K to collagen fiber. Then, the selected additives were applied to the enzymatic unhairing process for bovine hide. The results showed that a suitable concentration of metal ions (Cu (II), Fe (III) and Al (III)) and organic additives (salicylate, laurate, adipate, gallate and epicatechin (ECG)) could diminish approximately 35% of the hydrolytic activity of proteinase K to collagen fibers. Then, the additives were applied for the bovine hide enzymatic unhairing process. Hydroxyproline determination in the unhairing float shows that applying additives could reduce collagen hydrolysis. The morphology results showed that the grain damage could be significantly reduced with the addition of the screened additives in the proteinase K enzymatic unhairing system, whereas the addition of ECG and gallate significantly slowed down the unhairing speed. This outcome provides new potential to reduce the risk of grain damage in enzymatic unhairing process. Graphical abstract

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Effects and Mechanism of Metal Ions on Enzymatic Hydrolysis of Wheat Straw after Pretreatment

Cited by 15 publications

References 0 publications

Overcoming biomass recalcitrance by synergistic pretreatment of mechanical activation and metal salt for enhancing enzymatic conversion of lignocellulose

Overcoming biomass recalcitrance by synergistic pretreatment of mechanical activation and metal salt for enhancing enzymatic conversion of lignocellulose

Toward sustainable processes of pretreatment technologies of lignocellulosic biomass for enzymatic production of biofuels and chemicals: A review

Screening of additives to reduce grain damage risk on unhairing by proteinase K

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