Effects and Mechanisms of Alkali Recycling and Ozone Recycling on Enzymatic Conversion in Alkali Combined with Ozone Pretreatment of Corn Stover

Zhao, Zhezhen; Zhang, Jiaming; Li, Yiming; Li, Fēi; Liu, Ping

doi:10.1007/s12010-020-03425-4

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…High lignin degradation of 95.7% and 64.1% was obtained for EFB 0.3 mm and EFB 0.5 mm, respectively, while high TRS yield of 0.84 and 0.67 was obtained for EFB 0.3 mm and EFB 0.5 mm, respectively. This is due to high inhibitory compounds were generated by degraded sugar with low molecular lignin compounds at higher ozone concentration [40]. The increase in TRS yield was attributed to the disrupted lignin structures, which allowed cellulose to be more accessible for subsequent hydrolysis steps.…”

Section: Effect Of Process Parameter; Moisture Content Reaction Time and Ozone Concentrationmentioning

confidence: 99%

Chemical and Structural Changes of Ozonated Empty Fruit Bunch (EFB) in a Ribbon-Mixer Reactor

Rasid

Shamjuddin

Amin

2021

Bull. Chem. React. Eng. Catal.

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Agricultural wastes especially empty fruit bunch (EFB) are abundantly available to be utilized as a feedstock for biochemical synthesis or biofuel production. The components of the waste include lignin, hemicellulose and cellulose. Cellulose, the polymer of glucose, is the active component for producing bio-based chemicals. However, it is difficult to isolate cellulose since lignin, the most outer layer in the waste is recalcitrant. Therefore, the agricultural wastes need to be pre-treated prior to downstream processing. The aim of this study was to investigate the effect of ozone pretreatment on lignin degradation and total reducing sugar (TRS) yield. EFB was pre-treated using ozone gas in a ribbon-mixer reactor. The chemical and structural changes of ozonated EFB were analysed. The highest delignification obtained were 95.7 wt.% and TRS yield was enhanced to 84.9% at a moisture content of 40 wt.% with 60 g/m3 ozone concentration within one hour of reaction time. Both NMR and FTIR spectra conferred major peaks inferring higher lignin degradation could be achieved using ozonolysis. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

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Section: Effect Of Process Parameter; Moisture Content Reaction Time and Ozone Concentrationmentioning

confidence: 99%

Chemical and Structural Changes of Ozonated Empty Fruit Bunch (EFB) in a Ribbon-Mixer Reactor

Rasid

Shamjuddin

Amin

2021

Bull. Chem. React. Eng. Catal.

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“…Solvation reaction can dissolve part of lignin into liquid components. Meanwhile, saponification of intermolecular ester bonds reduces the strength of hydrogen bonds between hemicellulose and lignin, and increases porosity and surface area, causing the expansion of the lignocellulosic biomass and makes it easier for enzymes to access (31,35). NaOH pretreatment improved cellulose digestibility and it was more effective in the solubilization of lignin, exhibiting less dissolution of cellulose and hemicellulose than acid and oxidant processes (36).…”

Section: Comparison Analysis Of Different Treatments Combined With Fenton On Lignocellulose Enzymaticmentioning

confidence: 99%

Effect and optimization of NaOH combined with Fenton pretreatment conditions on enzymatic hydrolysis of poplar sawdust

Lü

et al. 2021

Chem. Pap.

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In this study, the effects and mechanism of pretreatments of three types of chemical reagents combined with Fenton on poplar sawdust were studied and the optimization of enzymatic hydrolysis conditions was conducted using response surface methodology. The results showed that cellulase and hemicellulase had the best hydrolysis effect after NaOH-Fenton pretreatment, which were 63.73% and 29.29%, respectively. The optimal process of poplar substrate was to react in 1% NaOH at 100 ℃ for 1 h, then placed in the Fenton reaction system of 0.2 mmol Fe 2+ and 25 mmol H2O2 for 7 h, and finally subjected to enzymatic hydrolysis for 72 h at 52 ℃, with a liquid-solid ratio of 33 and 15 μL/g of β-glycosidase. Under this condition, the enzymatic hydrolysis rates of cellulase and hemicellulase reached 86.65% and 43.9%, respectively. In conclusion, the combination of NaOH and Fenton pretreatment can effectively promote the enzymatic hydrolysis of poplar sawdust, which has great potential in the production of cellulosic ethanol.

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Good Feasibility of Ozone-Microwave Treatment as a Sterilization Technology to Extend the Edible Life of Candied Fruit as a Post-processed Fresh Fruit Product

Huang,

Ni,

Xie

et al. 2024

Food Bioprocess Technol

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In order to solve the problem of infestation of fresh fruit after making them into candied fruit and the quality degradation caused by the infestation, a combined ozone-microwave sterilization method was used to treat four candied fruit molds.Response surface optimization analysis showed that when the ozone infusion time was at 10 min and with 560 W microwave power for 20 s, the inhibition rates of the four candied fruit molds S1, S2, X1, and H1 were 100%, 94.28%, 100%, and 100%, respectively. Moreover, according to the characterization of morphology, DNA electrophoretic profiles, and nucleic acid/protein leakage test, the integrity of the mold cell membrane was destroyed after the combined ozone-microwave treatment because of the thermal and nonthermal effects of microwave and the strongly oxidizing properties of ozone. The molds were dead because of the efflux of intracellular nucleic acid and protein and the degradation of DNA, which influenced normal cellular metabolism. Quality identification showed a better expected quality of candied fruit.

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Effects and Mechanisms of Alkali Recycling and Ozone Recycling on Enzymatic Conversion in Alkali Combined with Ozone Pretreatment of Corn Stover

Cited by 7 publications

References 36 publications

Chemical and Structural Changes of Ozonated Empty Fruit Bunch (EFB) in a Ribbon-Mixer Reactor

Chemical and Structural Changes of Ozonated Empty Fruit Bunch (EFB) in a Ribbon-Mixer Reactor

Effect and optimization of NaOH combined with Fenton pretreatment conditions on enzymatic hydrolysis of poplar sawdust

Good Feasibility of Ozone-Microwave Treatment as a Sterilization Technology to Extend the Edible Life of Candied Fruit as a Post-processed Fresh Fruit Product

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