Effects and Mechanisms of Cage versus Floor Rearing System on Goose Growth Performance and Immune Status

Tang, Bincheng; Hu, Shenqiang; Zhang, Xin; Ouyang, Qingyuan; Qing, Enhua; Wang, Wanxia; Hu, Jiwei; Li, Liang; Wang, Jiwen

doi:10.3390/ani13162682

Cited by 1 publication

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References 36 publications

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“…The GO enrichment analysis showed that the DEGs identified in spleens of each goose breed between different weeks of age were mostly enriched in the terms related to organ morphogenesis and development, such as nucleus and extracellular space. Similarly, several previous studies have reported that the age has major effects on the development of the goose spleen by regulating cell differentiation ( 31 , 32 ). Besides, the DEGs identified in spleens of the same age between two goose breeds were mostly enriched in the GO terms related to the structure and ion transport of plasma membrane.…”

Section: Discussionmentioning

confidence: 52%

Comparative transcriptomics analysis identifies crucial genes and pathways during goose spleen development

Hu,

Song,

et al. 2024

Front. Immunol.

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As the largest peripheral lymphoid organ in poultry, the spleen plays an essential role in regulating the body’s immune capacity. However, compared with chickens and ducks, information about the age- and breed-related changes in the goose spleen remains scarce. In this study, we systematically analyzed and compared the age-dependent changes in the morphological, histological, and transcriptomic characteristics between Landes goose (LG; Anser anser) and Sichuan White goose (SWG; Anser cygnoides). The results showed a gradual increase in the splenic weights for both LG and SWG until week 10, while their splenic organ indexes reached the peak at week 6. Meanwhile, the splenic histological indexes of both goose breeds continuously increased with age, reaching the highest levels at week 30. The red pulp (RP) area was significantly higher in SWG than in LG at week 0, while the splenic corpuscle (AL) diameter was significantly larger in LG than in SWG at week 30. At the transcriptomic level, a total of 1710 and 1266 differentially expressed genes (DEGs) between week 0 and week 30 were identified in spleens of LG and SWG, respectively. Meanwhile, a total of 911 and 808 DEGs in spleens between LG and SWG were identified at weeks 0 and 30, respectively. Both GO and KEGG enrichment analysis showed that the age-related DEGs of LG or SWG were dominantly enriched in the Cell cycle, TGF−beta signaling, and Wnt signaling pathways, while most of the breed-related DEGs were enriched in the Neuroactive ligand−receptor interaction, Cytokine−cytokine receptor interaction, ECM−receptor interaction, and metabolic pathways. Furthermore, through construction of protein-protein interaction networks using significant DEGs, it was inferred that three hub genes including BUB1, BUB1B, and TTK could play crucial roles in regulating age-dependent goose spleen development while GRIA2, GRIA4, and RYR2 could be crucial for the breed-specific goose spleen development. These data provide novel insights into the splenic developmental differences between Chinese and European domestic geese, and the identified crucial pathways and genes are helpful for a better understanding of the mechanisms regulating goose immune functions.

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Section: Discussionmentioning

confidence: 52%