Effects and mechanisms of Eps8 on the biological behaviour of malignant tumours (Review)

Luo, Kaili; Zhang, Lei; Yuan, Ling; Zhou, Hongyu; Yang, Hongjiang; Luo, Min; Chen, Qing

doi:10.3892/or.2021.7927

Cited by 8 publications

(4 citation statements)

References 118 publications

(220 reference statements)

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“…Proteins of the epidermal growth factor substrate 8 (EPS8) family can regulate actin dynamics directly through capping or bundling, or indirectly through enhancement of Rac GTPase function (Hertzog et al, 2010; Luo et al, 2021). EPS8 is primarily localized to the tips of tall stereocilia, where it is thought to act mainly as an actin bundler.…”

Section: Microvillar Actin Cytoskeletonmentioning

confidence: 99%

The morphological and functional diversity of apical microvilli

et al. 2022

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Sensory neurons use specialized apical processes to perceive external stimuli and monitor internal body conditions. The apical apparatus can include cilia, microvilli, or both, and is adapted for the functions of the particular cell type. Photoreceptors detect light through a large, modified cilium (outer segment), that is supported by a surrounding ring of microvilli-like calyceal processes (CPs). Although first reported 150 years ago, CPs remain poorly understood. As a basis for future study, we therefore conducted a review of existing literature about sensory cell microvilli, which can act either as the primary sensory detector or as support for a cilia-based detector. While all microvilli are finger-like cellular protrusions with an actin core, the processes vary across cell types in size, number, arrangement, dynamics, and function. We summarize the current state of knowledge about CPs and the characteristics of the microvilli found on inner ear hair cells (stereocilia) and cerebral spinal fluid-contacting neurons, with comparisons to the brush border of the intestinal and renal epithelia. The structure, stability, and dynamics of the actin core are regulated by a complement of actin-binding proteins, which includes both common components and unique features when compared across cell types. Further, microvilli are often supported by lateral links, a glycocalyx, and a defined extracellular matrix, each adapted to the function and environment of the cell. Our comparison of microvillar features will inform further research into how CPs support photoreceptor function, and also provide a general basis for investigations into the structure and functions of apical microvilli found on sensory neurons.

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Section: Microvillar Actin Cytoskeletonmentioning

confidence: 99%

The morphological and functional diversity of apical microvilli

et al. 2022

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“… 33 Reduced expression in tumor tissue of CTNNA1 has been suggested to be a marker for early-stage ovarian epithelial cancers with poor prognosis, 34 while decreased protein expression in different ovarian cancer cell lines have been shown to either increase or decrease sensitivity to different chemotherapies. 35 A recent review 36 of the role of EPS8, including the EPS8L2 homolog, reports overexpression (as seen here) in a large variety of cancers, including ovarian cancer with involvement in tumorigenesis, tumor proliferation, migration, metastasis as well as drug resistance and poor prognosis. Although several candidate biomarkers were found with strong statistical support comparing cases and controls in the screening scenario, we found no strong univariate candidates separating the benign tumors from malignant.…”

Section: Discussionmentioning

confidence: 63%

Toward ovarian cancer screening with protein biomarkers using dried, self-sampled cervico-vaginal fluid

Hedlund Lindberg,

Widgren,

Ivansson

et al. 2024

iScience

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“…Multiple genes whose products act as cytoskeleton regulatory molecules were also upregulated, such as RHOF , WASL , CAP1 , MYL12B , LMTK3 , WDR , RHOD , SEPT2 , and WASF2 ( Figure 2 ). Moreover, the gene EPS8 , which codes for a receptor adaptor protein ( 92 ), and the EZR gene ( Figure 2 ) were upregulated. The AZR gene codes for the ezrin, a protein that belongs to the ERM (Ezrin–Radixin–Moesin) family and functions as a cross-linker between the actin cytoskeleton and the plasma membrane.…”

Section: Resultsmentioning

confidence: 99%

Pulmonary Mesenchymal Stem Cells in Mild Cases of COVID-19 Are Dedicated to Proliferation; In Severe Cases, They Control Inflammation, Make Cell Dispersion, and Tissue Regeneration

et al. 2022

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Mesenchymal stem cells (MSCs) are multipotent adult stem cells present in virtually all tissues; they have potent self-renewal capacity and differentiate into multiple cell types. For many reasons, these cells are a promising therapeutic alternative to treat patients with severe COVID-19 and pulmonary post-COVID sequelae. These cells are not only essential for tissue regeneration; they can also alter the pulmonary environment through the paracrine secretion of several mediators. They can control or promote inflammation, induce other stem cells differentiation, restrain the virus load, and much more. In this work, we performed single-cell RNA-seq data analysis of MSCs in bronchoalveolar lavage samples from control individuals and COVID-19 patients with mild and severe clinical conditions. When we compared samples from mild cases with control individuals, most genes transcriptionally upregulated in COVID-19 were involved in cell proliferation. However, a new set of genes with distinct biological functions was upregulated when we compared severely affected with mild COVID-19 patients. In this analysis, the cells upregulated genes related to cell dispersion/migration and induced the γ-activated sequence (GAS) genes, probably triggered by IFNGR1 and IFNGR2. Then, IRF-1 was upregulated, one of the GAS target genes, leading to the interferon-stimulated response (ISR) and the overexpression of many signature target genes. The MSCs also upregulated genes involved in the mesenchymal-epithelial transition, virus control, cell chemotaxis, and used the cytoplasmic RNA danger sensors RIG-1, MDA5, and PKR. In a non-comparative analysis, we observed that MSCs from severe cases do not express many NF-κB upstream receptors, such as Toll-like (TLRs) TLR-3, -7, and -8; tumor necrosis factor (TNFR1 or TNFR2), RANK, CD40, and IL-1R1. Indeed, many NF-κB inhibitors were upregulated, including PPP2CB, OPTN, NFKBIA, and FHL2, suggesting that MSCs do not play a role in the “cytokine storm” observed. Therefore, lung MSCs in COVID-19 sense immune danger and act protectively in concert with the pulmonary environment, confirming their therapeutic potential in cell-based therapy for COVID-19. The transcription of MSCs senescence markers is discussed.

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Effects and mechanisms of Eps8 on the biological behaviour of malignant tumours (Review)

Cited by 8 publications

References 118 publications

The morphological and functional diversity of apical microvilli

The morphological and functional diversity of apical microvilli

Toward ovarian cancer screening with protein biomarkers using dried, self-sampled cervico-vaginal fluid

Pulmonary Mesenchymal Stem Cells in Mild Cases of COVID-19 Are Dedicated to Proliferation; In Severe Cases, They Control Inflammation, Make Cell Dispersion, and Tissue Regeneration

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