Effects and Molecular Mechanism of Single-Nucleotide Polymorphisms of MEG3 on Porcine Skeletal Muscle Development

Yang, Rui; Liu, Yinuo; Cheng, Yi; Wang, Chunli; Song, Jié; Lu, Guanhong; Feng, Tianqi; Wang, Siyao; Sun, Xiaotong; Meng, Jilun; Hao, Linlin

doi:10.3389/fgene.2021.607910

Cited by 6 publications

(9 citation statements)

References 47 publications

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“…The SNP rs1859168, which is located in the HOTTIP (HOXA distal transcript), a lncRNA transcribed from the 5 tip of the HOXA locus, affects the expression and function of HOTTIP by reducing free energy to influence RNA secondary structure (Minotti et al, 2018). The SNP rs4081134 located in the lncRNA MEG3, influences the RNA secondary structure by decreasing free energy, which consequently affects the function of MEG3 (Yang et al, 2021). According to the RNAfold prediction, we found that plncMGPF haplotype H1 is more stable than H3.…”

Section: Discussionmentioning

confidence: 99%

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Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability

Zhao

Hou

et al. 2021

Front. Cell Dev. Biol.

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lncMGPF is a novel positive regulator of myogenic differentiation, muscle growth and regeneration in mouse, pig, and human. But whether natural mutations within lncMGPF gene regulate animal meat production traits is unclear. In this study, ten single nucleotide polymorphisms (SNPs) of pig lncMGPF (plncMGPF) gene were identified among commercial pig breeds and Chinese local pig breeds. These SNPs are highly linked and constructed into multiple haplotypes, and haplotype ATTCATGTTC (H1) mainly exists in commercial pig breeds while haplotype GCCTGCACCT (H3) is more frequent in Chinese local pig breeds. Association analysis indicated that all SNPs are significantly associated with the backfat thickness and loin muscle area (P < 0.05), respectively, and homologous H1 individuals have higher loin muscle area and lower backfat thickness than H3 pigs. Bioinformatics and functional analysis showed that haplotype H1 has a longer half-life and more stable RNA secondary structure than haplotype H3. plncMGPF haplotype H1 has stronger effects on pig primary myogenic progenitor cells differentiation and muscle growth than haplotype H3. Further experiments showed that two SNPs (rs81403974 and rs325492834) function together to confer plncMGPF stability and function. Our observation suggested that the SNPs in lncMGPF can change the RNA stabilities and lncMGPF function, thereby affecting the porcine meat production traits.

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Section: Discussionmentioning

confidence: 99%

“…Some studies have found that SNPs may change the secondary structure of lncRNA (Yang et al, 2021). We speculated that the SNPs of plncMGPF may change the secondary structures of plncMGPF.…”

Section: The Haplotypes Of Pig Lncmgpf Significantly Affect the Secondary Structures And The Rna Stabilitymentioning

confidence: 90%

Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability

Zhao

Hou

et al. 2021

Front. Cell Dev. Biol.

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“…Previous studies have shown that exonic SNPs may affect the secondary structures of lncRNAs (Yang et al., 2021). To explore whether the three exonic SNPs of pSYISL gene could change the secondary structures of pSYISL , the RNAfold web server was used to detect the RNA secondary structures and stability of pSYISL haplotypes H1, H2 and H3.…”

Section: Resultsmentioning

confidence: 99%

“…The SNPs of lncRNAs can affect the function of lncRNAs and play an important role in cancer (Feng Hua et 2018;Shah et al, 2018;Tian et al, Yan et al, 2021), muscle growth (Al-Tobasei et al, 2017;Yang et al, 2021;Zhou et al, 2018), muscle fiber girth and body weight (Attaway et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

Functional SNPs in SYISL promoter significantly affect muscle fiber density and muscle traits in pigs

Lv,

Peng,

et al. 2023

Animal Genetics

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Our previous studies showed that SYISL is a negative regulator of muscle growth and regeneration in mice, pigs and humans. SYISL knockout resulted in an increase in the density of muscle fibers and muscle growth. However, it is unclear whether there are natural mutations in pig SYNPO2 intron sense‐overlapping lncRNA (pSYISL) that affect the expression of pSYISL and muscle growth traits. In this study, three SNPs in exons and six SNPs within the promoter of pSYISL were identified. Association analysis showed that the two SNPs in exons are significantly associated with loin muscle area (p < 0.05); the six SNPs in the promoter that show complete linkage are significantly associated with live backfat thickness and live loin muscle area in American Large White pigs. Bioinformatics and luciferase reporter assays as well as in vitro binding experiments indicated that the mutation of SNP rs702045770 (g.539G>A) leads to the loss of YY1 binding to the promoter, thus affecting the expression level of pSYISL, and we found that Jiangshan Black pigs with genotype GG have a higher expression level of pSYISL than genotype AA individuals, but the muscle fiber density was significantly lower than in genotype AA individuals. Furthermore, the association analysis showed that the carcass backfat thickness of genotype GG of SNP rs702045770 was significantly higher than that of other genotypes in (Pietrain × Duroc) × (Landrace × Yorkshire) crossbred pigs (p < 0.05). The glycolytic potential of genotype GG was significantly higher than that of other genotypes (p < 0.05). These results provide novel insight into the identification of functional SNPs in non‐coding genomic regions.

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“…The qRT-PCR reaction was performed by one cycle of 45°C for 15 min, one cycle of 95°C for 2 min, 40 cycles of 95°C for 10 sec, 60°C for 15 sec and 71 cycles of 60°C for 15 sec. Primers were as follows: cdk4, F 5'-TTC GAG CAT CCC AAT GTT GTC -3' and R 5'-GTC TCG ATG AAC GAT GCA GTT G -3'; cdk6, F 5'-TTG GCT TTG GTG GGT AGT TCT-3' and R 5'-TGA ATG TCG GGT AGG AAG ATT G-3'; cyclin D1, F 5'-TGC ATC TAC ACC GAC AAC TCC A -3' and R 5'-GTT GGA AAT GAA CTT CAC GTC TGT-3'; gapdh, F 5'-ATG GTG AAG GTC GGA GTG AAC-3' and R 5'-CTC GCT CCT GGA AGA TGG T-3' (Yang et al, 2021); cyclin A, F 5'-GAT TTA CAT CTT AGA AAA CAA AGG-3' and R 5'-GGT GAT CCC GCC GTC CAC T-3' (Tang et al, 2010). The 2 −ΔΔCt method was applied for Ct value analysis (Livak, Schmittgen, 2001).…”

Section: Quantitative Real-time Rt-pcrmentioning

confidence: 99%

Simulated microgravity altered the cell cycle progression of porcine granulosa cells

Dai

Sơn

Long

2022

Vietnam J. Biotechnol.

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Microgravity has been shown to markedly affect reproduction in humans and animals, especially reproductive organs such as the ovaries. Granulosa cells are one of the important components of the ovary, playing an important role in supporting oocyte maturation and fertilization. However, the effects of microgravity on granulosa cells have not been well characterized. This study aimed to assess the effects of simulated microgravity (SMG) on the cell cycle progression of porcine granulosa cells (pGCs). The pGCs were induced SMG for 72 h by Gravite® simulator, while cells of the control group were treated in normal conditions. Cell cycle analysis revealed that SMG condition induced an increase of the ratio of pGCs in the G0/G1 phase, leading to the cell cycle arrest phase, while the ratio of pGCs in the G2/M phase was decreased. There was no difference in the cell ratio of the S phase between the control group and the SMG group. Real-time RT-PCR analysis indicated that the expression of cdk4 and cdk6 transcripts of pGCs from the SMG group was lower than the control group. This down-regulation was also observed cyclin A and cyclin D1 transcript expression in pGCs from the SMG group. Immunostaining displayed the lower exhibition of microfilament and microtubule in pGCs from the SMG group comparing to the control group. Western blot analysis showed that the expression of β-actin and α-tubulin was reduced in pGCs from the SMG group. These changes contributed to the alteration of cytoskeletal structure, including microfilaments and microtubules, which affect cell division. These results revealed that the SMG condition induced changes in the cell cycle progression of pGCs.

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Effects and Molecular Mechanism of Single-Nucleotide Polymorphisms of MEG3 on Porcine Skeletal Muscle Development

Cited by 6 publications

References 47 publications

Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability

Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability

Functional SNPs in SYISL promoter significantly affect muscle fiber density and muscle traits in pigs

Simulated microgravity altered the cell cycle progression of porcine granulosa cells

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