Effects and molecular mechanisms of Achyranthes bidentata polypeptide k on proliferation of Schwann cells

Tang, Leili; Zhang, Min; Liu, Xingyu; Zhu, Ye; Chen, Xin; Zhong, Jingfei; Li, Meiyuan

doi:10.21037/atm-21-5181

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Achyranthes bidentata could be a well-known Chinese complementary pharmaceutical with numerous capacities and impacts. A survey showed that the Achyranthes bidentata polypeptides at 0.5 μg/mL for 24 h could significantly promote the proliferation of primary SCs cultured in vitro at different times ( 61 ). The co-culture of SCs and adrenal pheochromocytoma (PC-12) cells showed a high cell proliferation rate and axonal expansion ( 62 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

et al. 2023

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Background: Silkworm products were first used by physicians more than 8500 years ago, in the early Neolithic period. In Persian medicine, silkworm extract has several uses for treating and preventing neurological, cardiac, and liver diseases. Mature silkworms (Bombyx mori) and their pupae contain a variety of growth factors and proteins that can be used in many repair processes, including nerve regeneration. Objectives: The study aimed to evaluate the effects of mature silkworm (Bombyx mori), and silkworm pupae extract on Schwann cell proliferation and axon growth. Methods: Silkworm (Bombyx mori) and silkworm pupae extracts were prepared. Then, the concentration and type of amino acids and proteins in the extracts were evaluated by Bradford assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and liquid chromatograph-mass spectrometer (LC-MS/MS). Also, the regenerative potential of extracts for improving Schwann cell proliferation and axon growth was examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, electron microscopy, and NeuroFilament-200 (NF-200) immunostaining. Results: According to the results of the Bradford test, the total protein content of pupae extract was almost twice that of mature worm extract. Also, SDS-PAGE analysis revealed numerous proteins and growth factors, such as bombyrin and laminin, in extracts that are involved in the repair of the nervous system. In accordance with Bradford's results, the evaluation of extracts using LC-MS/MS revealed that the number of amino acids in pupae extract was higher than in mature silkworm extract. It was found that the proliferation of Schwann cells at a concentration of 0.25 mg/mL in both extracts was higher than the concentrations of 0.01 and 0.05 mg/mL. When using both extracts on dorsal root ganglion (DRGs), an increase in length and number was observed in axons. Conclusions: The findings of this study demonstrated that extracts obtained from silkworms, especially pupae, can play an effective role in Schwann cell proliferation and axonal growth, which can be strong evidence for nerve regeneration, and, consequently, repairing peripheral nerve damage.

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Section: Discussionmentioning

confidence: 99%

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

et al. 2023

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Achyranthes bidentate extracts protect the IL-1β-induced osteoarthritis of SW1353 chondrocytes

Xiong,

Huang,

Chang

et al. 2023

Journal of Bioscience and Bioengineering

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Evaluation and Comparison of the Effects of mature Silkworm (Bombyx Mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An in vitro study

Khosropanah

Zolbin

Kajbafzadeh

et al. 2022

Preprint

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Mature silkworms (Bombyx mori) and their pupae contain a variety of growth factors and proteins such as bombyrin that can be used in many repair processes. Due to its appropriate repair qualities, the silkworm has been explored for use in nerve regeneration. First silkworm (Bombyx Mori) and silkworm Pupae Extracts were prepared. Then the concentration and type of amino acids and proteins in the extracts were evaluated by Bradford assay, SDS-page, and LC-MS/MS. Also, the regenerative potential of extracts for improving Schwann cell proliferation and axon growth was examined by MTT, SEM, and NF200 immunostaining. According to the results of the Bradford test, the total protein content of pupae extract was almost twice that of mature worm extract. Also, SDS-PAGE analysis revealed numerous proteins and growth factors such as bombyrin and laminin in extracts that are involved in the repair of the nervous system. Based on LC-MS/MS results, the number of amino acids in pupae extract was higher than in mature silkworm extract. It was found that the proliferation of Schwann cells at a concentration of 0.25 in both extracts was higher than the concentrations of 0.01 and 0.05. When using both extracts on DRGs, an increase in length and number was observed in axons.The findings of this study demonstrated that extracts obtained from adult silkworms, especially pupae, can play an effective role in Schwann cell proliferation and axonal growth, which can be strong evidence for nerve regeneration and consequently repairing peripheral nerve damage.

show abstract

Effects and molecular mechanisms of Achyranthes bidentata polypeptide k on proliferation of Schwann cells

Cited by 3 publications

References 25 publications

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

Achyranthes bidentate extracts protect the IL-1β-induced osteoarthritis of SW1353 chondrocytes

Evaluation and Comparison of the Effects of mature Silkworm (Bombyx Mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An in vitro study

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