Effects in vitro of l-glutamate and kainic acid on the ATPase activities of synaptosomal membranes from different areas of rat brain

Szkudlarek, Jolanta; Lachowicz, Lilla; R, Wojtkowiak

doi:10.1016/0304-3940(86)90279-x

Cited by 11 publications

(3 citation statements)

References 24 publications

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“…The extracellular Ca 2+ concentration in the medium was 58 ~VI, as demonstrated using the Calcium Ionophore 1 -Cocktail A [34]. Na+,K+-ATPase activity was measured using a linked reaction in the presence of an excess of pymvate kinase, lactate dehydrogenase, and phosphoenolpymvate [20,31]. Mg2+-ATPase activity was assessed in the presence of strophanthin G [20].…”

Section: Methodsmentioning

confidence: 99%

The use of antioxidants to prevent glutamate-induced derangement of calcium ion metabolism in rat cerebral cortex synaptosomes

Аврова

Shestak

Захарова

et al. 2000

Neurosci Behav Physiol

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Glutamate is shown to induce increases in intracellular Ca2+ concentrations ([Ca2+]i), increases in 45Ca2+ influx, decreases in the activity of Na+,K+-ATPase activity, and activation of the Na+/Ca2+ exchanger in rat cerebral cortex synaptosomes. NMDA receptor antagonists virtually prevented these effects. Preincubation of synaptosomes with alpha-tocopherol, superoxide dismutase, and ganglioside GM1 normalized [Ca2+]i, 45Ca2+ influx, and Na+,K+-ATPase activity in rat cerebral cortex synaptosomes exposed to glutamate. Glutamate and GM1 activated the Na+/K+ exchanger, and their effects were additive. Calcium ions entering cerebral cortex nerve cells via NMDA receptors during exposure to high glutamate concentrations appeared to be only the trigger for the processes activating free-radical reactions. Activation of these reactions led to increases in Ca2+ influx into cells, decreases in Na+,K+-ATPase activity, and significant increases in [Ca2+]i, though this could be prevented by antioxidants and gangliosides.

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Section: Methodsmentioning

confidence: 99%

The use of antioxidants to prevent glutamate-induced derangement of calcium ion metabolism in rat cerebral cortex synaptosomes

Аврова

Shestak

Захарова

et al. 2000

Neurosci Behav Physiol

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“…For the nerve cell membrane, Na + , K + -ATPase is the enzyme without which it is impossible to maintain the stability of ion gradients and membrane potential [61]. Numerous ion pumps in the neural membrane, which create and maintain a critical level The bending modulus of POPC bilayers modified by 1 mmol/L of L-Glu decreases by nearly ~25% compared to its value obtained for bare POPC bilayers.…”

Section: Discussionmentioning

confidence: 96%

“…For the nerve cell membrane, Na + , K + -ATPase is the enzyme without which it is impossible to maintain the stability of ion gradients and membrane potential [61]. Numerous ion pumps in the neural membrane, which create and maintain a critical level of membrane potential and ion gradients across the membrane, use ATP as an energy source, mainly of mitochondrial origin.…”

Section: Discussionmentioning

confidence: 99%

Surface Properties of Synaptosomes in the Presence of L-Glutamic and Kainic Acids: In Vitro Alteration of the ATPase and Acetylcholinesterase Activities

2021

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Morphologically and functionally identical to brain synapses, the nerve ending particles synaptosomes are biochemically derived membrane structures responsible for the transmission of neural information. Their surface and mechanical properties, measured in vitro, provide useful information about the functional activity of synapses in the brain in vivo. Glutamate and kainic acid are of particular interest because of their role in brain pathology (including causing seizure, migraine, ischemic stroke, aneurysmal subarachnoid hemorrhage, intracerebral hematoma, traumatic brain injury and stroke). The effects of the excitatory neurotransmitter L-glutamic acid and its agonist kainic acid are tested on Na+, K+-ATPase and Mg2+-ATPase activities in synaptic membranes prepared from the cerebral cortex of rat brain tissue. The surface parameters of synaptosome preparations from the cerebral cortex in the presence of L-glutamic and kainic acids are studied by microelectrophoresis for the first time. The studied neurotransmitters promote a significant increase in the electrophoretic mobility and surface electrical charge of synaptosomes at 1–4 h after isolation. The measured decrease in the bending modulus of model bimolecular membranes composed of monounsaturated lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine provides evidence for softer membranes in the presence of L-glutamate. Kainic acid does not affect membrane mechanical stability even at ten-fold higher concentrations. Both the L-glutamic and kainic acids reduce acetylcholinesterase activity and deviation from the normal functions of neurotransmission in synapses is presumed. The presented results regarding the modulation of the enzyme activity of synaptic membranes and surface properties of synaptosomes are expected by biochemical and biophysical studies to contribute to the elucidation of the molecular mechanisms of neurotransmitters/agonists’ action on membranes.

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Effects of two-vessel forebrain ischemia and of administration of indomethacin and quinacrine on Na+, K+-ATPase activity in various rat brain areas

Molchanova

Moskvin

Захарова

et al. 2005

J Evol Biochem Phys

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Effects in vitro of l-glutamate and kainic acid on the ATPase activities of synaptosomal membranes from different areas of rat brain

Cited by 11 publications

References 24 publications

The use of antioxidants to prevent glutamate-induced derangement of calcium ion metabolism in rat cerebral cortex synaptosomes

The use of antioxidants to prevent glutamate-induced derangement of calcium ion metabolism in rat cerebral cortex synaptosomes

Surface Properties of Synaptosomes in the Presence of L-Glutamic and Kainic Acids: In Vitro Alteration of the ATPase and Acetylcholinesterase Activities

Effects of two-vessel forebrain ischemia and of administration of indomethacin and quinacrine on Na+, K+-ATPase activity in various rat brain areas

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