Effects of 1,2-propanediol on the cytoskeletal organization of the mouse oocyte

Joly, Claire; Bchini, Olfa; Boulekbache, Habib; Testart, J; Maro, Bernard

doi:10.1093/oxfordjournals.humrep.a137654

Cited by 70 publications

(35 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PROH has been reported to be useful in the cryopreservation of mammalian embryos (4), but it seems to be less effective with mammalian oocytes. Murayama et al (5) using PROH, has reported less than 20% of cryopreserved mouse oocytes survived after thawing, whilst Joly et al (6) reported that 1.5 M PROH caused mouse oocytes to form cytoplasmic blebs.…”

Section: Introductionmentioning

confidence: 99%

“…It may be due to the change in nature of the zona pellucida or zona hardening by premature release of cortical granules (15)(16)(17), loss of the plasma membrane selective permeability (18), or extensive disorganization of ooplasm and damage to the spindle apparatus by disorganization of microtubules and microfilaments during freezing and thawing (6,19,20). Carroll et al (21) observed that the freezing-thawing procedure induced changes in the zona pellucida of oocytes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Developmental potential and ultrastructural injuries of metaphase II (MII) mouse oocytes after slow freezing or vitrification

Valojerdi

Salehnia

2005

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Developmental potential and ultrastructural injuries of metaphase II (MII) mouse oocytes after slow freezing or vitrification

Valojerdi

Salehnia

2005

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…It is well known that the visual changes in spindles and abnormal chromosomes alignment after vitrification were linked with functional effects of oocytes on fertilization and development (O'Neil et al 1998;Almeida and Bolton 1993). A sufficient restoration of cytoskeleton after incubation would be critical for fertilization events (Joly et al 1992). In our study, rates of fertilization, developing to four-cell embryos and blastocyst, were significantly higher in 2 h incubation group than those of in 1 and 3 h incubation groups.…”

Section: Discussionmentioning

confidence: 41%

Factors affecting the survival, fertilization, and embryonic development of mouse oocytes after vitrification using glass capillaries

Tan

Song

Liu

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Cryopreservation of mammalian oocytes is an important way to provide a steady source of materials for research and practice of parthenogenetic activation, in vitro fertilization, and nuclear transfer. However, oocytes cryopreservation has not been common used, as there still are some problems waiting to be solved on the repeatability, safety, and validity. Then, it is necessary to investigate the damage occurred from vitrification and find a way to avoid or repair it. In this study, mouse mature oocytes were firstly pretreated in different equilibrium media, such as 5% ethylene glycol (EG) + 5% dimethyl sulfoxide (DMSO), 10% EG + 10% DMSO, and 15% EG + 15% DMSO in TCM199 supplemented with 20% fetal calf serum (FCS), for 1, 3, and 5 min, respectively, and then oocytes were transferred into vitrification solution (20% EG, 20% DMSO, 0.3 M sucrose, and 20% FCS in TCM199, M2, Dulbecco's phosphate buffered saline, and 0.9% saline medium, respectively) and immediately loaded into glass capillaries to be plunged into liquid nitrogen. After storage from 1 h to 1 wk, they were diluted in stepwise sucrose solutions. The surviving oocytes were stained for cortical granule, meiotic spindles, and chromosomes. Oocytes without treatments were used as controls. The results showed that oocytes pretreated in 5% EG +5% DMSO group for 3-5 min or in 10% EG + 10% DMSO group for 1-3 min were better than other treatments. Oocytes vitrified in TCM199 as basic medium showed higher survival and better subsequent embryonic development than other groups. When the concentration of FCS in vitrification solution reduced below 15%, the rates of survival, fertilization, and developing to blastocyst declined dramatically. The inner diameter (0.6 mm) of glass capillaries and amount of vitrification solution (1-3 microl) achieved more rapid cooling and warming and so reduce the injury to oocytes. Cropreservation led to the exocytosis of cortical granule of oocytes (about 10%) and serious disturbance of microtubules and chromosomes. With 2 h incubation, the microtubules could repolymerize and the rate of fertilization in vitro was much higher than those of 1 and 3 h incubation groups. In conclusion, the protection of basic medium and FCS to oocytes during cryopreservation and sufficient cooling and warming rates using glass capillaries have profound effects on oocytes survival and subsequent embryonic development competence. The appropriate time for fertilization in vitro may be related to the recovery of spindles after incubation and avoiding ageing in the whole process.

show abstract

“…Many publications on problems of mammalian oocyte cryopreservation contain information on the negative effects of low temperature including the cytoskeleton depolimerization effect of permeable cryoprotectants (Johnson and Pickering, 1987;Aigner et al, 1992;Joly et al, 1992). We believe that the negative effect of cooling on porcine oocytes may be explained by way of the effect of cooling lipids on cytoskeletal structures.…”

Section: Discussionmentioning

confidence: 93%

Vitrification of GV-porcine oocytes by 1-, 2-, and 7-step saturation in ethylene glycol

Isachenko¹,

Isachenko²,

Pérez-Sánchez³

et al. 1998

J. Anim. Feed Sci.

View full text Add to dashboard Cite

Three experiments were conducted to investigate the effect of 1 -, 2-and 7-step saturation with cryoprotectant on vitrification of germinal vesicle porcine oocytes to M-II stage. In Experiment I non-cooled cumulus-oocyte complexes (COCs) were equilibrated in ethylene glycol (EG) using a single-step or step-wise protocol, EG was removed by single-step or step-wise procedure with all manipulations being performed at 42°C. In Experiment II non-cooled COCs were equilibrated in ethylene glycol (EG) using the single-step or step-wise protocol with further EG removal by the single-step or step-wise procedure and pretreatment with Cytochalasin B. In experiment III, performed taking into account the results of Experiments I and II, a total of 177 COCs were divided into three treatment and one control groups. All treatment COCs were vitrified after pretreatment with Cytochalasin B (5|ig/ml at 38.5°C for 10-15 min), direct plunging into liquid nitrogen, thawing for 5 sec in a waterbath at 50°C, removal of the cryoprotectant by exposure to 1M, 0.75M, 0.5M, 0.25M for 4, 2, 2, and 2 min respectively at 40-42°C. Group 1 COCs (n=52) were cryopreserved after exposition to 40% EG for 4 min. Group 2 COCs (n = 47) were exposed to 25% EG for 5 min and then to 40% EG for 30 sec prior to vitrification. Group 3 COCs (n = 48) were equilibrated over 13 min using a series of EG concentrations 5, 10, 15, 20,25, 30, and 40% EG for 5,2,2,2, 1, 0.5, and 0.5 min, respectively. Group 4 COCs (n =30) were control oocytes (not exposed to EG and not frozen). After thawing and passage through the sucrose series (treatment) or without treatment (control) COCs were cultured for 48 h in TCM-199 with 10% heat-inactivated follicular fluid and lug/ml FSH in 5% C0 2 at 39°C. The developmental capacity (progression to M-II stage) as assessed by aceto-orcein staining was 0,4.2, 29.2, and 86.6%, respectively (P, 2 < 0.01; P M 34 2 4 <0.001).

show abstract

Effects of 1,2-propanediol on the cytoskeletal organization of the mouse oocyte

Cited by 70 publications

References 0 publications

Developmental potential and ultrastructural injuries of metaphase II (MII) mouse oocytes after slow freezing or vitrification

Developmental potential and ultrastructural injuries of metaphase II (MII) mouse oocytes after slow freezing or vitrification

Factors affecting the survival, fertilization, and embryonic development of mouse oocytes after vitrification using glass capillaries

Vitrification of GV-porcine oocytes by 1-, 2-, and 7-step saturation in ethylene glycol

Contact Info

Product

Resources

About