EFFECTS OF 17 Α-Methyltestosterone AND TRIBULUS TERRESTRIS EXTRACT ON SEX RATIO AND GONADS HISTOLOGY OF RED TILAPIA HYBRID

Amer, M. A.; Zaki, F. G.; Tahoun, Al‐Azab; Said, Mohamed M.

doi:10.21608/ejnf.2021.210947

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…Our finding is in contrast with previous studies that reported 100% males in commercial tilapia farms [ 55 , 56 ], which was possibly due to the various nursing techniques and climates. Importantly, red tilapia fry in the T 6 group produced 98.5% males, which is a significantly higher percentage of male tilapia compared with previous studies that found less than 70% sex-reversed males after the administration of 30 mg/kg of conventional MT for 28 days [ 6 , 57 ]. Our findings also confirmed that lipid nanoparticles could be a promising delivery system for androgen hormones.…”

Section: Discussionmentioning

confidence: 59%

Masculinization of Red Tilapia (Oreochromis spp.) Using 17α-Methyltestosterone-Loaded Alkyl Polyglucosides Integrated into Nanostructured Lipid Carriers

Yostawonkul

Kitiyodom

Supchukun

et al. 2023

Animals

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The aim of the present study was to optimize a masculinization platform for the production of all-male red tilapia fry by oral administration of 30 and 60 ppm of MT and alkyl polyglucoside nanostructured lipid carriers (APG-NLC) loaded with MT, respectively, for 14 and 21 days. The characterization, encapsulation efficiency and release kinetics of MT in lipid-based nanoparticles were assessed in vitro. The results showed that the MT-loaded nanoparticles were spherical, ranging from 80 to 125 nm in size, and had a negative charge with a narrow particle distribution. The APG-NLC loaded with MT provided higher physical stability and encapsulation efficacy than the NLC. The release rate constants of MT from MT-NLC and MT-APG-NLC were higher than those of free MT, which is insoluble in aqueous media. There was no significant difference in survival between the fish administered MT or the those fed orally with MT-APG-NLC fish. According to the logistic regression analysis, the sex reversal efficacy of MT-APG-NLC (30 ppm) and MT (60 ppm), resulted in significantly higher numbers of males after 21 days of treatment compared with the controls. The production cost of MT-APG-NLC (30 ppm) after 21 days of treatment was reduced by 32.9% compared with the conventional MT treatment group (60 ppm). In all the treatments, the length–weight relationship (LWR) showed negatively allomeric growth behavior (b < 3), with a relative condition factor (Kn) of more than 1. Therefore, MT-APG-NLC (30 ppm) would seem to be a promising, cost-effective way to reduce the dose of MT used for the masculinization of farmed red tilapia.

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Section: Discussionmentioning

confidence: 59%

Masculinization of Red Tilapia (Oreochromis spp.) Using 17α-Methyltestosterone-Loaded Alkyl Polyglucosides Integrated into Nanostructured Lipid Carriers

Yostawonkul

Kitiyodom

Supchukun

et al. 2023

Animals

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“…There is not always a linear relationship between increasing the MT dosage and improving the masculinization rate. In fact, treatments with excessive doses, which in our study seems to be the case of treatments 40 and 60 mg/kg, can lead to disruptions in gonadal development or sterility (Devlin and Nagahama, 2002) and could also lead to reduced masculinization, or in some instances, it could induce paradoxical feminization (Demska-Zakes and Zakes, 1997;Marjani et al, 2009;Amer et al, 2021, Karaket et al, 2023.…”

Section: Discussionmentioning

confidence: 68%

Effect of dosage of orally administered 17α-methyltestosterone on sex reversion of the yellowtail tetra Astyanax lacustris (Lütken, 1875)

et al. 2023

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The females of yellowtail tetra (Astyanax lacustris), known as the freshwater sardine, are approximately 1.33 times larger than males, and thus, all-female monosex culture would increase production and reduce size variability. The present work aimed to identify the optimal dose of 17α-methyltestosterone (MT) to be used in the masculinization of A. lacustris for indirect sex reversal. Three different concentrations of MT (20, 40, and 60 mg/kg of feed in the diet) were fed to the fry for 30 days. Thirty adult individuals from each treatment, including the control (0 mg MT/kg), were evaluated for gonadal development, morphological and histological sexual identification, zootechnical performance, and the possible genotoxic effect caused by prolonged exposure to MT. MT significantly (P<0.01) affected the differentiation of the gonads, with the presence of possible inhibitory effects in all treatments. Intersex individuals were present in the 20 and 60 mg MT/kg treatments. All treatments were able to masculinize A. lacustris and the treatment with the lowest hormone concentration produced the highest percentage of males 76.7%, while the control had 46.7% males. The presence of erythrocyte nuclear alterations indicated a possible cytotoxic effect of MT in treatments 40 and 60 mg MT/kg, however, the use of the hormone did not affect the growth and the survival of the individuals. Thus, the use of MT is a viable option for obtaining neomales as a first step into the production of all-female progenies.

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“…Parallelly, the androgen 17-methyltestosterone (17-MT) occurs naturally in fish (MT). It is a synthetic androgen that promotes both sexual maturation in men and muscular growth in men [13,14]. The main commercial application of monosex populations is hormonal and selective breeding, which has received a lot of attention.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary aromatase inhibitors on masculinization of rosy barb (Pethia conchonius): Evidence from growth, coloration and gonado-physiological changes

Lal,

Biswas,

Singh

et al. 2023

PLoS ONE

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The objective of this study was to reveal the growth, colouration and gonado-physiological changes due to the exogenous aromatase inhibitor (AIs) in an ornamental fish. 17α-methyltestosterone (MT) and letrozole (LET) were used as potential AIs. The AI were supplemented with a gel-based feed (LET: 50, 100, 150 and MT: 12.5, 25, 37.5 mg/kg feed) in Rosy barb, Pethia conchonius fry. The fishes were reared in a 45-L glass tank using AI treated gel-based feed for 3 months. Growth in AI-based diets was reduced but the reduction was minimal compared to the control. At 25 mg/kg feed of 17 MT, the highest male proportion (84.72% 6.05%) was recorded, which was significantly higher (P≤0.05) than other groups. L*, a*, and b* values showed that 17α-MT-fed groups had brighter coloration (P≤0.05). Histological sections showed that LET-17α-MT suppressed ovarian development, causing atretic oocytes. Testicular development was unaffected. 25 mg/kg-treated feed increased SOD, CAT, GST, and GPX. The AI (MT) at 25 mg/kg gel-based feed could therefore be utilised for musculinization without impacting growth, colour, and antioxidant activity of rosy barb, which serves the entire male population in the ornamental fish sector.

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EFFECTS OF 17 Α-Methyltestosterone AND TRIBULUS TERRESTRIS EXTRACT ON SEX RATIO AND GONADS HISTOLOGY OF RED TILAPIA HYBRID

Cited by 3 publications

References 25 publications

Masculinization of Red Tilapia (Oreochromis spp.) Using 17α-Methyltestosterone-Loaded Alkyl Polyglucosides Integrated into Nanostructured Lipid Carriers

Masculinization of Red Tilapia (Oreochromis spp.) Using 17α-Methyltestosterone-Loaded Alkyl Polyglucosides Integrated into Nanostructured Lipid Carriers

Effect of dosage of orally administered 17α-methyltestosterone on sex reversion of the yellowtail tetra Astyanax lacustris (Lütken, 1875)

Effects of dietary aromatase inhibitors on masculinization of rosy barb (Pethia conchonius): Evidence from growth, coloration and gonado-physiological changes

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