Effects of 17α-ethynylestradiol on sexual development of male western mosquitofish (Gambusia affinis)

Angus, Robert A.; Stanko, Jason P.; Jenkins, Ronald L.; Watson, R. Douglas

doi:10.1016/j.cca.2005.03.008

Cited by 28 publications

(31 citation statements)

References 35 publications

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“…Poeciliids have been widely used to investigate the in vivo effects of reproductively active endocrine disrupting compounds (e.g., Doyle and Lim, 2002, Angus et al, 2005, Rawson et al, 2008. They are less commonly used to examine the effects and exposure to POPs.…”

Section: Use Of Mosquitofish In Determination Of In Vivo and In Situ mentioning

confidence: 99%

Bioactivity of POPs and their effects in mosquitofish in Sydney Olympic Park, Australia

Rawson

Tremblay

Warne

et al. 2009

Science of The Total Environment

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a b s t r a c tThe site of the 2000 Olympic Games (Sydney Olympic Park (SOP), Sydney, Australia) was contaminated by persistent organic pollutants (POPs) prior to remediation in the 1990s. This study investigates the bioactivity of POPs in the sediment and water of wetlands across SOP by in vitro 2,3,7,8-TCDD equivalence (TCDDeq) measurement (H4IIE cell line bioassay). Further, it examines whether disturbance of these sediments is likely to mobilise ligands for this receptor into the water column. Exposure to aryl hydrocarbon receptor (AhR) ligands was measured in vivo using hepatic cytochrome P4501A (CYP1A) induction (EROD) in the mosquitofish (Gambusia holbrooki). Aqueous TCDDeq ranged from 0.013 to 0.057 pM in SOP wetlands which was significantly (p b 0.05) less that in urban reference sites. These concentrations were not correlated to physical or chemical characteristics of the wetlands. In the sediments, TCDDeq ranged from 0.0016 to 7.06 µg/kg and these were not significantly (p ≥ 0.05) different to that measured in urban reference sites. Simulated disturbance of small quantities of sediment in water samples significantly (p b 0.05) increased the levels of TCDDeq measured in the water. Sediment TCDDeq was correlated to sediment ΣPAH concentration in 2006 and sediment ΣPCB, ΣDDT concentrations and fine sediment grain size in 2005. While fish at one SOP wetland had hepatic EROD activity elevated above the estimated basal level for this species, these were at the lower end of the range measured in urban impacted, non-remediated wetlands. EROD activity was positively correlated with both the sediment ΣPCB load and aqueous TCDDeq. Increased catchment size was correlated with increased EROD activity suggesting an even spread of POPs throughout the residential areas of the Sydney metropolitan area. The concentration of bioactive POPs in the wetlands of SOP is therefore low relative to urban reference sites demonstrating the ongoing success of the remediation program..

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Section: Use Of Mosquitofish In Determination Of In Vivo and In Situ mentioning

confidence: 99%

Bioactivity of POPs and their effects in mosquitofish in Sydney Olympic Park, Australia

Rawson

Tremblay

Warne

et al. 2009

Science of The Total Environment

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“…This is because as Sumpter and Johnson (2005) recorded 'one animal's poison may not be another's'. However, G. holbrooki has been widely used in this role (e.g., Angus et al 2005;Batty and Lim 1999;Doyle and Lim 2002).…”

Section: Resultsmentioning

confidence: 99%

“…An alternative approach to assessing endocrine disruption in aquatic systems is to use an indicator species. Mosquito fish Gambusia holbrooki, considered to be a useful sentinel species (Pyke 2005), has been shown to respond to estrogens in laboratory studies of 17β-Estradiol (Doyle and Lim 2002) and 17β-Ethynylestradiol (Angus et al 2005). They have also been observed to be an indicator of endocrine disruption in aquatic systems (Batty and Lim 1999).…”

Section: Introductionmentioning

confidence: 98%

Answering questions on the impact of recycled water on wildlife usingGambusia holbrookias a surrogate

Norris

Burgin

2011

Australian Zoologist

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The disruption of endocrine systems due to environmental contaminants potentially impacts on developmental, behavioural, regulatory, and reproductive systems of wildlife. A major source of exposure of wildlife (terrestrial and aquatic) to endocrine disrupting compounds is through contact with contaminated surface waters. Current testing routines in aquatic systems have not been designed specifically to assess endocrine disruption properties or, alternatively, methods tend not to be fully developed. An alternative approach is to use a response sensitive to these chemicals such as gonopodium length in the pest species Gambusia holbrooki as an indicator. This species was used as a surrogate for native aquatic species to assess endocrine disruption in wetlands that are used for the storage of stormwater or treated sewage effluent. These were compared with adjacent wetlands used for watering stock (farm dams) that were not contaminated with these pollutants. Deformities in the mosquito fish were found that were consistent with endocrine disruption caused by sex steroids and/ or their mimics in the first in the sequence of each pollution type but not in farm dams. We concluded that the first wetland in the sequence of recycling stormwater or treated sewage effluent could cause detrimental effects to wildlife but not in subsequent wetlands in a sequence of water storage systems. Since all waters are moved through at least three wetlands before being used for irrigation, or released into the river system, the impact on wildlife is likely to be minimal beyond the first wetlands.

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“…Batty and Lim 1999;Angus et al 2001Angus et al , 2002Angus et al , 2005Lim 2002, 2005). These fish are abundant and widespread, have the ability to survive in polluted environments, and hence are useful sentinel species (Pyke 2005).…”

Section: Introductionmentioning

confidence: 97%

“…Exposure to 17β-estradiol (Doyle and Lim 2002) and 17α-ethynylestradiol lead to a reduction in the length of the male gonopodium (Angus et al 2005), while 4-nonylphenol leads to incomplete gonopodium elongation (Drèze et al 2000). Overall, there is therefore a reduction in the length of the male gonopodium in the presence of estrogenic, xenoestrogenic, and/or anti-androgenic activity only during development (Doyle and Lim 2005).…”

Section: Introductionmentioning

confidence: 97%

Apparent Rapid Loss of Endocrine Disruptors from Wetlands Used to Store Either Tertiary Treated Sewage Effluent or Stormwater Runoff

Norris

Burgin

2010

Water Air Soil Pollut

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The disruption of endocrine systems due to environmental contaminants potentially impacts on the developmental, behavioural, regulatory and reproductive systems of animals. A major source of exposure of animals (terrestrial and aquatic) to endocrine-disrupting compounds is through contact with contaminated surface waters contaminated with sewage effluent and/or stormwater discharge. We studied the response to endocrine-disrupting compounds of Gambusia holbrooki mosquito fish resident of wetlands that were used for the storage of either treated sewage effluent or stormwater runoff. We found that fish from wetlands that received polluted waters directly from the source (treated sewage effluent or stormwater runoff) demonstrated a morphological response consistent with endocrine disruption. In contrast, fish in the second in the series of wetlands that housed treated sewage effluent did not show evidence of such response. However, those from the second in the series of stormwater receiving wetlands did display a morphological response, although it could be considered milder than was observed in fish from the first in this effluent stream. Fish were also smaller in the wetland that received sewage effluent directly from the sewage treatment plant than elsewhere. Although the differences were not statistically significant, the average size of fish in the first in the series of stormwater wetlands were also small and second only to those in the first sewage effluent wetland.

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Effects of 17α-ethynylestradiol on sexual development of male western mosquitofish (Gambusia affinis)

Cited by 28 publications

References 35 publications

Bioactivity of POPs and their effects in mosquitofish in Sydney Olympic Park, Australia

Bioactivity of POPs and their effects in mosquitofish in Sydney Olympic Park, Australia

Answering questions on the impact of recycled water on wildlife usingGambusia holbrookias a surrogate

Apparent Rapid Loss of Endocrine Disruptors from Wetlands Used to Store Either Tertiary Treated Sewage Effluent or Stormwater Runoff

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