Effects of 17β-estradiol and growth factors on c-fos gene expression in endometrial epithelial cells in primary culture

Identification and Expression of a New Sulfhydryl Oxidase SOx-3 during the Cell Cycle and the Estrus Cycle in Uterine Cells

“…Glandular epithelial cells (GEC) and stromal endometrial cells (SC) were cultured at 37°C in an atmosphere of 5% CO 2 . Subconfluent GEC were obtained within 6 -7 days and were either made quiescent by serum depletion over a 60-h period (14) or subcultured.…”

“…It has been demonstrated that: (i) the cultured cells are estrogen-responsive (13); (ii) they can be made quiescent by serum depletion (14); (iii) quiescent cells are able to enter again the cell cycle under unspecific mitogen stimulation (i.e., fetal calf serum) (15); (iv) E 2 has no mitogenic effect but, in association with a protein synthesis inhibitor such as cycloheximide (Chx), it induces c-fos gene expression (14,16). In an effort to identify genes that may be estrogen-regulated in the same conditions as c-fos, a cDNA library has been constructed from GEC stimulated for 2 h with E 2 plus Chx.…”

Protooncogene c-fos Involvement in the Molecular Mechanism of Rat Brain Sexual Differentiation

“…In the pituitary estrogen induces c-fos in a cell-specific manner [52]. Furthermore, estrogens have been shown to induce c-fos in other tissues, such as the rat uterus [53], cultured guinea pig uterine epithelium [54], in the MCF-7 breast cancer cell line [55], and in both bone-resorbing osteoclasts and bone-forming osteoblasts [56, 57]. In these cases the induction of c-fos appears to be a direct effect of the hormone [36].…”

A Novel Early Estrogen-Regulated Gene gec1 Encodes a Protein Related to GABARAP

“…These motifs differ only by one residue from the consensus pattern [R or K -X(2)-D or E-X(3)-Y]. GABARAP, differently from GEC1, has a serine residue which could be phosphorylated by casein kinase II (16)(17)(18)(19) or by cAMP-dependent protein kinase (13)(14)(15)(16).…”

“…It has been demonstrated that the cultured cells are estrogen responsive (13) and that 17␤-estradiol (E 2 ) induces c-fos gene expression within 2 h when it acts in association with either epidermal growth factor plus insulin or a protein synthesis inhibitor such as cycloheximide (14,15). In an effort to identify genes that may be regulated by E 2 in the same conditions as c-fos, a cDNA library has been constructed from poly(A) ϩ RNAs extracted from GEC stimulated with E 2 (10 Ϫ8 M) plus cycloheximide (Chx, 10 g/ml) for 2 h using the gt10 cloning system.…”