Effects of 2,3′,4,4′5-pentachlorobiphenyl exposure during pregnancy on epigenetic imprinting and maturation of offspring’s oocytes in mice

He, Qi-Long; Wei, Xu-Yu; Han, Xiaoying; Zhou, Qian; Wang, Hai-Quan; Ding, Nai-Zheng; Meng, Xiao-Qian; Schatten, Heide; Sun, Qing‐Yuan; Liu, Shuzhen

doi:10.1007/s00204-019-02529-z

Cited by 19 publications

(7 citation statements)

References 57 publications

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“…Transcriptome RNA-seq process Total RNA was extracted via a TRK1001 Total RNA Purification Kit (LC Science, Houston, TX) according to the manufacturer's protocol [93]. The total RNA amount and purity were determined using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA), with RNA values > 7.0.…”

Section: Metabolite Profiling and Data Analysismentioning

confidence: 99%

“…AMPure XP beads were used to purify the double-stranded product, both T4 DNA polymerase and Klenow DNA polymerase were used to repair attachment of the sticky end of the DNA to the blunt end, A bases and linkers were added to the 3′ end, AMPureXP beads were used for fragment selection, and PCR amplification was ultimately performed to increase the final sequencing library. After the library was quantified, it was generated by an Illumina HiSeq 4000 instrument, and the sequencing read length was double-ended 2*150 bp (PE150) [93]; low-quality reads were removed. The raw sequence data are available under GEO Series accession number GSE114968.…”

Section: Metabolite Profiling and Data Analysismentioning

confidence: 99%

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Transcriptomic and metabolomic analyses reveal mechanisms of adaptation to salinity in which carbon and nitrogen metabolism is altered in sugar beet roots

et al. 2020

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Background: Beta vulgaris L. is one of the main sugar-producing crop species and is highly adaptable to saline soil. This study explored the alterations to the carbon and nitrogen metabolism mechanisms enabling the roots of sugar beet seedlings to adapt to salinity. Results: The ionome, metabolome, and transcriptome of the roots of sugar beet seedlings were evaluated after 1 day (short term) and 7 days (long term) of 300 mM Na + treatment. Salt stress caused reactive oxygen species (ROS) damage and ion toxicity in the roots. Interestingly, under salt stress, the increase in the Na + /K + ratio compared to the control ratio on day 7 was lower than that on day 1 in the roots. The transcriptomic results showed that a large number of differentially expressed genes (DEGs) were enriched in various metabolic pathways. A total of 1279 and 903 DEGs were identified on days 1 and 7, respectively, and were mapped mainly to 10 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Most of the genes were involved in carbon metabolism and amino acid (AA) biosynthesis. Furthermore, metabolomic analysis revealed that sucrose metabolism and the activity of the tricarboxylic acid (TCA) cycle increased in response to salt stress. After 1 day of stress, the content of sucrose decreased, whereas the content of organic acids (OAs) such as L-malic acid and 2-oxoglutaric acid increased. After 7 days of salt stress, nitrogen-containing metabolites such as AAs, betaine, melatonin, and (S)-2-aminobutyric acid increased significantly. In addition, multiomic analysis revealed that the expression of the gene encoding xanthine dehydrogenase (XDH) was upregulated and that the expression of the gene encoding allantoinase (ALN) was significantly downregulated, resulting in a large accumulation of allantoin. Correlation analysis revealed that most genes were significantly related to only allantoin and xanthosine. Conclusions: Our study demonstrated that carbon and nitrogen metabolism was altered in the roots of sugar beet plants under salt stress. Nitrogen metabolism plays a major role in the late stages of salt stress. Allantoin, which is involved in the purine metabolic pathway, may be a key regulator of sugar beet salt tolerance.

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Section: Metabolite Profiling and Data Analysismentioning

confidence: 99%

Section: Metabolite Profiling and Data Analysismentioning

confidence: 99%

Transcriptomic and metabolomic analyses reveal mechanisms of adaptation to salinity in which carbon and nitrogen metabolism is altered in sugar beet roots

et al. 2020

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“…As one of the important markers of epigenetics, DNA methylation has been widely studied in recent years, and epigenetic imprinting also affects fetal growth in the uterus and postnatal development (Reik & Walter, 2001). It has been confirmed that exposure to PCB118 at low doses during pregnancy affects the DNA methylation pattern of imprinted genes in the oocytes of mice offspring and changes the relative expression levels of Dnmts (He et al, 2019). Genomic imprinting has been shown to play an important regulatory role in mammalian spermatogenesis (Hartmann, Bergmann, Bohle, Weidner, & Steger, 2006; Marques, Carvalho, Sousa, & Barros, 2004).…”

Section: Discussionmentioning

confidence: 93%

Effects of intrauterine exposure to 2,3′,4,4′,5‐pentachlorobiphenyl on the reproductive system and sperm epigenetic imprinting of male offspring

Lyu

Wang

et al. 2020

J of Applied Toxicology

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Polychlorinated biphenyls (PCBs) are a class of persistent organic environmental pollutants with a total of 209 homologs. The homolog 2,3′,4,4′,5‐pentachlorobiphenyl (PCB118) is one of the most important dioxin‐like PCBs and is highly toxic. PCB118 can accumulate in human tissues, serum and breast milk, which leads to direct exposure of the fetus during development. In the present study, pregnant mice were exposed to 0, 20 and 100 μg/kg/day of PCB118 during the stage of fetal primordial germ cell migration. Compared with the control group, we found morphological alterations of the seminiferous tubules and a higher sperm deformity rate in the male offspring in the treatment groups. Furthermore, the methylation patterns in the treatment groups of the imprinted genes H19 and Gtl2 in the sperm were altered in the male offspring. We also characterized the disturbance of the expression levels of DNA methyltransferase 1 (Dnmt1), Dnmt3a, Dnmt3b, Dnmt3l, and Uhrf1. The results indicated that intrauterine exposure to low doses of PCB118 could significantly damage the reproductive health of the male offspring. Therefore, attention should be paid to the adverse effects of PCB118 exposure during pregnancy on the reproductive system of male offspring.

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“…Comparison of the 55 DEGs identified in F1–D1 and F1–D2 ovaries with those identified after exposure to other EDCs showed that 32 (61%) were shared ( Supplementary Table S9 ). Specifically, Arhgap29 and Cyfip2 (genes involved in regulating ECM and actin filament organization) were deregulated also after exposure to bisphenol S (BPS) [ 90 ], Bicd1 (microtubule cytoskeletal organization), and Cyfip2 , Nlrp4f , Crabp2 , and Rnf19b (actin filament organization) were deregulated also after exposure to pentachlorobiphenyl (PCB) [ 91 ]. Nlrp4f , PadI6 , Tcf21 (ECM/actin/microtubule organization), Zp2 (the transcriptional repressor in the circadian system that targets Cyp19a1 expression and inhibits estrogen synthesis ( Nr1d1 )) [ 92 ], and Nlrp14 (oocyte and spermatogenic marker) [ 93 ] were upregulated also after exposure to deoxynivalenol (DON).…”

Section: Resultsmentioning

confidence: 99%

Cocktails of NSAIDs and 17α Ethinylestradiol at Environmentally Relevant Doses in Drinking Water Alter Puberty Onset in Mice Intergenerationally

Philibert

Déjardin

Girard

et al. 2023

IJMS

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Non-steroidal anti-inflammatory drugs (NSAIDs) and 17α-ethinyl-estradiol (EE2) are among the most relevant endocrine-disrupting pharmaceuticals found in the environment, particularly in surface and drinking water due to their incomplete removal via wastewater treatment plants. Exposure of pregnant mice to NSAID therapeutic doses during the sex determination period has a negative impact on gonadal development and fertility in adults; however, the effects of their chronic exposure at lower doses are unknown. In this study, we investigated the impact of chronic exposure to a mixture containing ibuprofen, 2hydroxy-ibuprofen, diclofenac, and EE2 at two environmentally relevant doses (added to the drinking water from fetal life until puberty) on the reproductive tract in F1 exposed mice and their F2 offspring. In F1 animals, exposure delayed male puberty and accelerated female puberty. In post-pubertal F1 testes and ovaries, differentiation/maturation of the different gonad cell types was altered, and some of these modifications were observed also in the non-exposed F2 generation. Transcriptomic analysis of post-pubertal testes and ovaries of F1 (exposed) and F2 animals revealed significant changes in gene expression profiles and enriched pathways, particularly the inflammasome, metabolism and extracellular matrix pathways, compared with controls (non-exposed). This suggested that exposure to these drug cocktails has an intergenerational impact. The identified Adverse Outcome Pathway (AOP) networks for NSAIDs and EE2, at doses that are relevant to everyday human exposure, will improve the AOP network of the human reproductive system development concerning endocrine disruptor chemicals. It may serve to identify other putative endocrine disruptors for mammalian species based on the expression of biomarkers.

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Effects of 2,3′,4,4′5-pentachlorobiphenyl exposure during pregnancy on epigenetic imprinting and maturation of offspring’s oocytes in mice

Cited by 19 publications

References 57 publications

Transcriptomic and metabolomic analyses reveal mechanisms of adaptation to salinity in which carbon and nitrogen metabolism is altered in sugar beet roots

Transcriptomic and metabolomic analyses reveal mechanisms of adaptation to salinity in which carbon and nitrogen metabolism is altered in sugar beet roots

Effects of intrauterine exposure to 2,3′,4,4′,5‐pentachlorobiphenyl on the reproductive system and sperm epigenetic imprinting of male offspring

Cocktails of NSAIDs and 17α Ethinylestradiol at Environmentally Relevant Doses in Drinking Water Alter Puberty Onset in Mice Intergenerationally

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