Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on secretion of steroids and STAR, HSD3B and CYP19A1 mRNA expression in chicken ovarian follicles

Expression of aryl hydrocarbon receptor 1 (AHR1), AHR1 nuclear translocator 1 (ARNT1) and CYP1 family monooxygenase mRNAs and their activity in chicken ovarian follicles following in vitro exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

“…Previously, we demonstrated that TCDD disturbs the function of the ovary by decreasing the basal and LH-stimulated secretion of sex steroids (Sechman et al, 2014). In the present study, we decided to assess the transcription of the AHR receptor in chicken ovarian follicles.…”

“…The dose of oLH was applied after the earlier in vitro studies (Nitta et al, 1991;Sechman et al, 2009Sechman et al, , 2011 while the main concentration of TCDD (i.e. 10 nM) was established on the basis of the dose-response curve from our previous studies (Sechman et al, 2014). The incubation was performed in 6 repetitions (n = 6) at 39 C for 6 h in an atmosphere of 95% air and 5% CO 2 .…”

“…Our previous study revealed that TCDD can effectively affect chicken ovarian steroidogenesis by influencing sex steroids secretion and mRNA expression of genes involved in their synthesis (Sechman et al, 2014). Since TCDD reduced not only basal but also LH-stimulated steroidogenesis in these follicles, the present study was designed to evaluate the in vitro effects of TCDD and LH -the main stimulator of sex steroid hormone synthesis in chicken ovarian follicles (Nitta et al, 1991;Sechman et al, 2011), on expression of genes encoding AHR1 and ARNT1 in white and yellowish prehierarchical follicles, and yellow preovulatory ones in the hen ovary.…”

Comparison of the in vitro effects of TCDD, PCB 126 and PCB 153 on thyroid-restricted gene expression and thyroid hormone secretion by the chicken thyroid gland

“…Accumulation of these compounds is mainly PCDDs is 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which exerts a diverse spectrum of negative biological responses, e.g. carcinogenesis, immunotoxicity, neurotoxicity, reproductive disorders, hepatotoxicity, and endocrine dysfunctions (Boverhof et al, 2006;Mandal, 2005;Sechman et al, 2014;Wang et al, 2011). Toxicity of PCBs depends on the number and location of chlorine atoms on the ring structure (Ottinger et al, 2014).…”

Gestational N-hexane inhalation alters the expression of genes related to ovarian hormone production and DNA methylation states in adult female F1 rat offspring

“…Previous studies have shown that hormesis can be caused by low-dose environmental endocrine exposure during gestation (Sechman et al, 2014;Ye et al, 2014). For example, exposure to prochloraz and tetrachlorodibenzo-p-dioxin (TCDD) during pregnancy led to increased serum progesterone levels in female offspring (Laier et al, 2006;Pesonen et al, 2006).…”