Effects of 2,3-Bis(4-hydroxyphenyl)-propionitrile on Induction of Polyovular Follicles in the Mouse Ovary

Satō, Tomomi; Kim, Hannah; Kakuta, Hanako; Iguchi, Taisen

doi:10.21873/invivo.11199

Cited by 2 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1c–d ). Moreover, some researchers found MOFs in rodents upon treatment with synthetic estrogenic hormones [ 27 ] or the estrogen receptor β agonist 2,3-bis(4-hydroxyphenyl)-propionitrile [ 28 ], as well as in ovaries of AQP8 knockout [ 19 ] or p27Kip1 mutant mice [ 29 ]. Furthermore, the frequency of MOFs differs among species or individuals, with possums showing high incidence of MOFs (e.g.…”

Section: Discussionmentioning

confidence: 99%

Unique morphological characteristics in the ovary of cotton rat (<i>Sigmodon hispidus</i>)

Islam

Ichii

Nakamura

et al. 2020

Journal of Reproduction and Development

View full text Add to dashboard Cite

Cotton rats (Sigmodon hispidus, CRs) are commonly used as animal models in biomedical research. However, the reproductive characteristics and ovarian development in the CRs has not been widely investigated. We have previously shown that female CRs, in particular, show several unique phenotypes associated with the urogenital system, such as chronic kidney disease and pyometra. Our investigation revealed unique morphologies in CR ovaries, particularly in oocytes. Cotton rat ovaries at 6-8 weeks of age were obtained from the Hokkaido Institute of Public Health, and their sections analyzed by light microscopy and transmission electron microscopy. Although the general histology and folliculogenesis of CR ovaries were similar to those of other experimental rodents, multi-oocyte follicles (MOFs) and double nucleated oocytes (DNOs) were also observed. Although MOFs were found at all stages of follicular development, a greater frequency of MOFs was observed in the primary and secondary stages. However, DNOs tended to be frequently observed in primordial follicles. Almost all MOF oocytes and a few DNOs possessed a clear zona pellucida, expressed DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 and Forkhead box protein 2, a representative marker of oocytes and follicular epithelial cells. Thus, our investigations revealed the unique phenotypes of the CR ovary. As MOFs and DNOs are occasionally observed in human patients with infertility, the CR would be a useful animal model to study for gaining a better understanding of folliculogenesis and oocytogenesis, as well as their abnormalities in humans and other animals.

show abstract

Section: Discussionmentioning

confidence: 99%

Unique morphological characteristics in the ovary of cotton rat (<i>Sigmodon hispidus</i>)

Islam

Ichii

Nakamura

et al. 2020

Journal of Reproduction and Development

View full text Add to dashboard Cite

show abstract

“…Rodents, especially laboratory mice belonging to the family Muridae, serve as an animal model for the study of folliculogenesis, but there have been no reports on the abundance of MOFs and DNOs. However, some researchers have found MOFs in rodents treated with synthetic estrogenic hormones [22] or the estrogen receptor β agonist 2,3bis(4-hydroxyphenyl)-propionitrile [23], as well as in the ovaries of AQP8 knockout [24] or p27Kip1 mutant mice [25]. We have previously reported several follicular disturbances, including the abundance of MOFs and DNOs in an inbred strain of SH, HIS/Hiph, maintained at the Hokkaido Institute of Public Health [3,4].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (Sigmodon hispidus)

Islam

Ichii

Nakamura

et al. 2021

Animals

View full text Add to dashboard Cite

Most mammalian ovarian follicles contain only a single oocyte having a single nucleus. However, two or more oocytes and nuclei are observed within one follicle and one oocyte, respectively, in several species, including cotton rat (CR, Sigmodon hispidus). The present study compared ovarian histology, focusing on folliculogenesis, between two inbred CR strains, HIS/Hiph and HIS/Mz. At 4 weeks of age, ovarian sections from both the strains were analyzed histologically. Multi-oocyte follicles (MOFs) and double-nucleated oocytes (DNOs) were observed in all stages of developing follicles in HIS/Hiph, whereas HIS/Mz had MOFs up to secondary stages and lacked DNOs. The estimated total follicles in HIS/Mz were almost half that of HIS/Hiph, but interstitial cells were well developed in HIS/Mz. Furthermore, immunostaining revealed no clear strain differences in the appearance of oocytes positive for Ki67, PCNA, and p63 in MOF or DNOs; no cell death was observed in these oocytes. Ultrastructural analysis revealed more abundant mitochondrial clouds in oocytes of HIS/Hiph than HIS/Mz. Thus, we clarified the strain differences in the CR ovary. These findings indicate that early events during folliculogenesis affect the unique ovarian phenotypes found in CRs, including MOFs or DNOs, and their strain differences.

show abstract

Effects of 2,3-Bis(4-hydroxyphenyl)-propionitrile on Induction of Polyovular Follicles in the Mouse Ovary

Abstract: Abstract. Background/Aim: Neonatal diethylstilbestrol (DES) treatment induces polyovular follicles (PFs)

Cited by 2 publications

References 34 publications

Unique morphological characteristics in the ovary of cotton rat (<i>Sigmodon hispidus</i>)

Unique morphological characteristics in the ovary of cotton rat (<i>Sigmodon hispidus</i>)

Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (Sigmodon hispidus)

Contact Info

Product

Resources

About