Effects of 2,4-Dimethoxyphenyl Biguanide on Glutathione System Activity in Rat Tissues in Brain Ischemia–Reperfusion

Сафонова, О. А.; Попова, Т. Н.; Панченко, Л. Ф.

doi:10.1007/s10517-011-1381-1

Cited by 6 publications

(2 citation statements)

References 8 publications

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“…Isocitrate dehydrogenase abundantly exists in neurons, microglia, and astrocytes [31]. It is down-regulated in Alzheimer's disease and stroke [32,33]. We showed a decrease of isocitrate dehydrogenase in glutamate-treated neonatal cerebral cortex.…”

Section: Discussionmentioning

confidence: 68%

Identification of proteins differentially expressed by glutamate treatment in cerebral cortex of neonatal rats

2019

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Glutamate leads to neuronal cell damage by generating neurotoxicity during brain development. The objective of this study is to identify proteins that differently expressed by glutamate treatment in neonatal cerebral cortex. Sprague-Dawley rat pups (post-natal day 7) were intraperitoneally injected with vehicle or glutamate (10 mg/kg). Brain tissues were isolated 4 h after drug treatment and fixed for morphological study. Moreover, cerebral cortices were collected for protein study. Two-dimensional gel electrophoresis and mass spectrometry were carried out to identify specific proteins. We observed severe histopathological changes in glutamate-exposed cerebral cortex. We identified various proteins that differentially expressed by glutamate exposure. Identified proteins were thioredoxin, peroxiredoxin 5, ubiquitin carboxy-terminal hydrolase L1, proteasome subunit alpha proteins, isocitrate dehydrogenase, and heat shock protein 60. Heat shock protein 60 was increased in glutamate exposed condition. However, other proteins were decreased in glutamate-treated animals. These proteins are related to anti-oxidant, protein degradation, metabolism, signal transduction, and anti-apoptotic function. Thus, our findings can suggest that glutamate leads to neonatal cerebral cortex damage by regulation of specific proteins that mediated with various functions.

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Section: Discussionmentioning

confidence: 68%

Identification of proteins differentially expressed by glutamate treatment in cerebral cortex of neonatal rats

2019

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“…The preservation of mitochondria function is considered to be an important factor in neuroplasticity and axonal regeneration during brain repair process [ 12 , 43 ]. Alzheimer’s disease and ischemic stroke patients showed a decrease in ICDH [ 6 , 53 ]. In this study, ICDH mRNA and protein expressions were decreased in ischemic cerebral cortex damage and EGCG treatment alleviated these decrease.…”

Section: Discussionmentioning

confidence: 99%

Identification of regulated proteins by epigallocatechin gallate treatment in an ischemic cerebral cortex animal model: a proteomics approach

2021

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Ischemic stroke is a fatal disease that has long-term disability. It induces excessive oxidative stress generation and cellular metabolic disorders, result in tissue damage. Epigallocatechin gallate (EGCG) is a naturally derived flavonoid with strong antioxidant property. We previously reported the neuroprotective effect of EGCG in ischemic stroke. The defensive mechanisms of stroke are very diverse and complex. This study investigated specific proteins that are regulated by EGCG treatment in the ischemic brain damage. Middle cerebral artery occlusion (MCAO) was performed to induce focal cerebral ischemia. EGCG (50 mg/kg) or vehicle was intraperitoneally administered just prior to MCAO. MCAO induced severe neurological deficits and disorders. EGCG treatment alleviated these neurological disorder and damage. Cerebral cortex was used for this study. Two-dimensional gel electrophoresis and mass spectrometry were performed to detect the proteins altered by EGCG. We identified various proteins that were changed between vehicle-and EGCG-treated animals. Among these proteins, isocitrate dehydrogenase, dynamin-like protein 1, and γ-enolase were decreased in vehicle-treated animals, while EGCG treatment prevented these decreases. However, pyridoxal-5'phosphate phosphatase and 60 kDa heat shock protein were increased in vehicle-treated animals with MCAO injury. EGCG treatment attenuated these increases. The changes in these proteins were confirmed by Western blot and reverse transcription-PCR analyses. These proteins were associated with cellular metabolism and neuronal regeneration. Thus, these findings can suggest that EGCG performs a defensive mechanism in ischemic damage by regulating specific proteins related to energy metabolism and neuronal protection.

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Protective effect of grape seed extract and orlistat co-treatment against stroke: Effect on oxidative stress and energy failure

Kadri

Ayed

Kadri

et al. 2021

Biomedicine & Pharmacotherapy

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Effects of 2,4-Dimethoxyphenyl Biguanide on Glutathione System Activity in Rat Tissues in Brain Ischemia–Reperfusion

Cited by 6 publications

References 8 publications

Identification of proteins differentially expressed by glutamate treatment in cerebral cortex of neonatal rats

Identification of proteins differentially expressed by glutamate treatment in cerebral cortex of neonatal rats

Identification of regulated proteins by epigallocatechin gallate treatment in an ischemic cerebral cortex animal model: a proteomics approach

Protective effect of grape seed extract and orlistat co-treatment against stroke: Effect on oxidative stress and energy failure

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