Effects of 2‐deoxy D‐glucose and other sugar analogues on acid production from sugars by human dental plaque bacteria

Roberts, Katherine; Hayes, Michael L.

doi:10.1111/j.1600-0722.1980.tb01215.x

Cited by 12 publications

(15 citation statements)

References 19 publications

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“…Plaque bacteria like mutans streptococci and lactobacilli use the phosphoenolpyruvate-phosphotransferase system for the uptake of mono-and disaccharides [Ajdic and Pham, 2007;Barrangou et al, 2006]. Therefore, it is not surprising that the K 0.5 values obtained for L. paracasei tolerans (11 mol l -1 ) and L. rhamnosus (14 mol l -1 ) are in accordance with comparable data for S. mutans (20 mol l -1 [Stoesser, 1984]) and dental plaque (12 mol l -1 [Stoesser, 1984], 20 mol l -1 [Roberts and Hayes, 1980]). In contrast, sugar uptake of C. albicans is dependent on the H + -ATPase system which is apparently activated at a 50-fold higher concentration of glucose.…”

Section: Discussionsupporting

confidence: 76%

Acid Production by Oral Strains of <i>Candida albicans</i> and Lactobacilli

Klinke¹,

et al. 2009

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Both Candida albicans and lactobacilli are common colonizers of carious lesions in children and adolescents. The purpose of this study is to compare the velocity of acid production between C. albicans and several Lactobacillus species at different pH levels and concentrations of glucose. Washed, pure resting-cell suspensions were obtained by culturing a total of 28 oral isolates comprising the species C. albicans, Lactobacillus rhamnosus, Lactobacillus paracasei paracasei, Lactobacillus paracasei tolerans and Lactobacillus delbrueckii lactis. Acid production from glucose was determined at a constant pH of 7.0, 5.5, 5.0 and 4.0 by repeated titrations with NaOH in an automated pH-stat system. Acid formation rates of yeast and lactobacilli proved to be similar at both neutral and low pH, while in a moderately acidic environment C. albicans produced less acid than the lactobacilli. Ion chromatographic analysis of the cell-free medium after titration revealed pyruvate to be the predominant organic acid anion secreted by C. albicans. The proportion of organic acids to overall acid production by the yeast was below 10% at neutral conditions, in contrast to 42–66% at pH 4.0. Compared to lactobacilli, yeast required a concentration of glucose that was about 50 times higher to allow acid production at half the maximum speed. Considering the clinical data in the literature about the frequency and proportions of microorganisms present in early childhood caries lesions, the contribution of oral lactobacilli as well as C. albicans to overall microbial acid formation appears to be important.

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Section: Discussionsupporting

confidence: 76%

Acid Production by Oral Strains of <i>Candida albicans</i> and Lactobacilli

Klinke¹,

et al. 2009

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“…Figure 1 shows that the rate of glucose incorporation was greater than the incorporation of other sugars. This is in agreement with the findings of Roberts and Hayes [1980] that glucose was the sug ar which gave the greatest rate of acid pro duction when added to plaque suspensions. In addition, the glucose concentration which gave half the maximum rate of acid produc tion (20 fiM) is of the same order as that which gave half the maximum rate of glu cose incorporation in the present work (41 iiM; fig.…”

Section: Incorporation Of Glucose Fructose Sucrose and 2-deoxyglucosesupporting

confidence: 82%

Glucose Incorporation by Human Dental Plaque Bacteria

Roberts¹,

Linder²,

Sund³

1981

Caries Res

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The incorporation of ¹⁴C-sugars was measured in dental plaque suspensions (pH 6.8) incubated at 37 °C. The initial rates of incorporation in 0.1 mM glucose, 0.1 mM fructose and 0.05 mM sucrose solutions were 1.21, 0.59 and 0.99 nmol hexose mg wet wt^––1min^-1, respectively. Incorporation of glucose showed saturation kinetics with 1/2 saturation occurring at 41 μM glucose. In suspensions of Streptococcus mitis, 111 saturation was at 25 μM glucose. Uptake of the non-utilisable glucose analogue 2-deoxyglucose was considerably slower than glucose in both plaque and S. mitis suspensions. Incorporation of glucose by S. mitis and plaque took place over a wide pH range and was inhibited in the presence of excess amounts of 2-deoxyglucose, mannose and 5-thioglucose. Inhibition also occurred in the presence of fluoride, sulphydryl reagents, arsenate, azide, proton-conducting uncouplers and the adenosine triphosphatase inhibitor N, N^I-dicyclohexylcarbodiimide. The inhibition studies indicate that glucose transport mechanisms other than phosphoenolpyruvate-dependent phosphotransferase systems are also present in oral bacteria.

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“…Since isomaltulose is only slightly decomposed by m icroorganisms (e.g. Streptococcus mutans, mitis, sanguis, salivarius) [von Gehring, 1973;Ohta and Takazoe, 1983] and plaque suspensions [Roberts and Hayes, 1980;Takazoe et al, 1982a], an occasional consum ption of isom altulose may not lower the plaque pH appreciably. According to Roberts and Hayes [1980], oligosaccharides containing the a -1-6 linkage (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that isomaltulose is slowly fermented by Streptococcus mutans and dental plaque suspensions [von Gehring, 1973;Roberts and Hayes, 1980;Ohta and Takazoe, 1983;Maki et al, 1983].…”

mentioning

confidence: 99%

Effect of Frequent Rinses with Isomaltulose (Palatinose®) Solution on Acid Production in Human Dental Plaque

Topitsoglou¹,

Sasaki²,

Takazoe³

et al. 1984

Caries Res

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Acid production from glucose and isomaltulose (Palatinose®) in dental plaque suspensions was studied before and after subjects rinsed their mouths 6 times daily for 6 weeks with a 15% w/v isomaltulose solution. The mean acid production from isomaltulose, expressed in percent of that from glucose, increased significantly (p < 0.01) after the rinsing period. In vivo plaque pH changes caused by a mouth rinse with 10 ml of a 15% w/v glucose or isomaltulose solution were determined also, before and after the rinsing period. The pH decreases from isomaltulose were significantly more pronounced after the rinsing period (p < 0.01) and the raw pH values were about 0.57 units lower than before (p < 0.001), but never below pH 6.2. Isomaltulose was considerably less acidogenic than glucose before as well as after the isomaltulose rinsing period (p < 0.001).

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Effects of 2‐deoxy D‐glucose and other sugar analogues on acid production from sugars by human dental plaque bacteria

Cited by 12 publications

References 19 publications

Acid Production by Oral Strains of <i>Candida albicans</i> and Lactobacilli

Acid Production by Oral Strains of <i>Candida albicans</i> and Lactobacilli

Glucose Incorporation by Human Dental Plaque Bacteria

Effect of Frequent Rinses with Isomaltulose (Palatinose®) Solution on Acid Production in Human Dental Plaque

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