Effects of 2-phenoxyethanol anaesthesia on juvenile meagre (Argyrosomus regius)

Abstract

This study was performed to evaluate the effective concentration of the anaesthetic 2-phenoxyethanol (2-PE) on juvenile (1.3 ± 0.03 g) meagre (Argyrosomus regius, Asso, 1801) and establish the LC 50 (through a series of exposure concentrations) and LT 50 of 2-PE at 20 ± 0.5°C, salinity 38 g · L )1 , pH 8.2-8.4 and dissolved oxygen >7 mg · L )1 . The induction time decreased and the recovery time increased with increasing concentrations. Conflicting results were found only in recovery time and there were no significant differences among the recovery times from all concentrations. The most suitable concentration of 2-PE was 0.3 ml · L )1 for about or over 15 min exposure time. The LC 50 and LT 50 for the 3-60 min exposure periods were estimated for juvenile meagre. The toxic effect of 2-PE on survival rates of A. regius juveniles increased depending on the exposure period. In addition, 2-phenoxyethanol LT 50 (median survival time) values, slope function (S) and lower and upper 95% confidence limits were estimated.

Efficiency of 2‐phenoxyethanol and Clove Oil for Reducing Handling Stress in Reared Meagre, Argyrosomus regius (Pisces: Sciaenidae)

“…For 2‐PE, anesthesia induction resulted in a negative exponential relationship between concentration and time for induction, whereas time for recovery followed a polynomial function where higher concentrations resulted in increased recovery times. This pattern of variation was identical to the pattern described for other marine fish species, like great sturgeon, Huso huso , white sea bream, Diplodus sargus , sharp snout sea bream, Diplodus puntazzo , and meagre, A. regius (Tsantilas et al ; Serezli et al ; Shaluei et al ). But for other species, like Senegalese sole, Solea senegalensis , an opposite response was observed where higher doses of 2‐PE resulted in a shorter recovery period (Weber et al ).…”

“…Fish progressed sequentially through the typical stages of anesthesia and recovery as described by Iwama et al (). The efficacy of these anesthetics for routine aquaculture practices have been studied in several fish species, some of which are listed in Table (Iwama et al ; Mylonas et al ; Tsantilas et al ; Weber et al ; Serezli et al ; Shaluei et al ). From these studies it can be concluded that anesthetic concentration changes with water temperature, fish species, and even fish size within the same species, reinforcing the importance of screening concentrations of anesthetics on new species.…”

“…According to Ross and Ross () an ideal anesthetic for fish should induce anesthesia in less than 3 min with total loss of equilibrium and muscle tone for easy handling. In addition, it should allow for a rapid recovery (less than 5 min), should leave low tissues residues, and be safe to users as well as cheap and easy to use (Tsantilas et al ; Weber et al ; Serezli et al ). In this study, the total loss of equilibrium and muscle tone is a condition equivalent to Stage AIII anesthesia described by Iwama et al ().…”

“…Fish progressed sequentially through the typical stages of anesthesia and recovery as described by Iwama et al (1989). The efficacy of these anesthetics for routine aquaculture practices have been studied in several fish species, some of which are listed in Table 3 (Iwama et al 1989;Mylonas et al 2005;Tsantilas et al 2006;Weber et al 2009;Serezli et al 2011;Shaluei et al 2012). From these studies it can be concluded that anesthetic Argyrosomus regius, juveniles (n = 6) maintained at 18 C and exposed to different doses of clove oil (COil).…”

Effects of different concentrations of 2-phenoxyethanol on primary and secondary stress responses in Persian sturgeon, Acipenser persicus

“…Sedative and anaethetic agents are used in aquaculture to induce anaethesia during research experiments, blood sampling, biometric measuring, transport, tagging, controlled reproduction and surgery procedures, in order to reduce physiologic disturbances and physical injuries (Weber et al., ). After MS222 (Tricaine methane sulfonate), 2‐phenoxyethanol (2‐PE) is the most commonly used anaethetic in aquaculture (Serezli et al., ; Topic Popovic et al., ).…”