Effects of 20-hydroxyecdysone and other hormones on egg development, and identification of a vitellin-binding protein in the ovary of the tick, Amblyomma hebraeum

Friesen, Kevin J.; Kaufman, W. Reuben

doi:10.1016/j.jinsphys.2004.03.008

Cited by 37 publications

(23 citation statements)

References 42 publications

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“…Subsequently, the injection of 200 and 1000 ng/tick of 20E into partially fed D. variabilis females led to higher vitellogenin protein levels, vitellin‐filled oocytes and ovary weights as compared with ticks that were injected with solvent (Thompson et al ., ); however, the doses that elicited a response represented 10 and 50 times the physiological levels found in D. variabilis haemolymph, while at the physiological equivalent dose (20 ng/tick) no response was observed 4 days after injection (Thompson et al ., ). Injection of 20HE was also an effector in eliciting increased vitellogenin production in partially fed adult females of the ixodid tick, Amblyomma hebraeum (Friesen & Kaufman, , ; Seixas et al ., ). Other studies have identified ecdysteroids in ticks and mites using various detection methods.…”

Section: Introductionmentioning

confidence: 99%

Three Halloween genes from the Varroa mite,Varroa destructor(Anderson & Trueman) and their expression during reproduction

Cabrera

Shirk

Evans

et al. 2014

Insect Molecular Biology

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The ecdysteroid biosynthetic pathway involves sequential enzymatic hydroxylations by a group of enzymes collectively known as Halloween gene proteins. Complete sequences for three Halloween genes, spook (Vdspo), disembodied (Vddib) and shade (Vdshd), were identified in varroa mites and sequenced. Phylogenetic analyses of predicted amino acid sequences for Halloween orthologues showed that the acarine orthologues were distantly associated with insect and crustacean clades indicating that acarine genes had more ancestral characters. The lack of orthologues or pseudogenes for remaining genes suggests these pathway elements had not evolved in ancestral arthropods. Vdspo transcript levels were highest in gut tissues, while Vddib transcript levels were highest in ovary-lyrate organs. In contrast, Vdshd transcript levels were lower overall but present in both gut and ovary-lyrate organs. All three transcripts were present in eggs removed from gravid female mites. A brood cell invasion assay was developed for acquiring synchronously staged mites. Mites within 4 h of entering a brood cell had transcript levels of all three that were not significantly different from mites on adult bees. These analyses suggest that varroa mites may be capable of modifying 7-dehydro-cholesterol precursor and hydroxylations of other steroid precursors, but whether the mites directly produce ecdysteroid precursors and products remains undetermined.

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Section: Introductionmentioning

confidence: 99%

Three Halloween genes from the Varroa mite,Varroa destructor(Anderson & Trueman) and their expression during reproduction

Cabrera

Shirk

Evans

et al. 2014

Insect Molecular Biology

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“…So the new peak appearance might be an indictor of the presence of VTG in male loach plasma. The sensitivity of this direct determination of VTG is very similar to the result of HPLC [13], but much lower than the ELISA method [8].…”

Section: Cze Analysis Of Vtgmentioning

confidence: 56%

“…The main techniques for VTG determination currently are enzyme linked immunosorbent assay (ELISA) [7][8][9], radioimmunoassay (RIA) [10,11], Western blots [12], HPLC [13] and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) [14]. In many related articles, immunoassays were applied in VTG analysis as the commonly used approach.…”

Section: Introductionmentioning

confidence: 99%

Separation and detection of vitellogenin in fish plasma by capillary zone electrophoresis

Song

Shao

et al. 2005

Journal of Chromatography B

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“…However, the specific process that triggers vitellogenin uptake has not been established. The most favored hypothesis is that a "vitellogenin capture factor" may exist (FRIESEN; KAUFMAN, 2004;SEIXAS et al, 2010). Thus, it can be suggested that this accumulation occurred through a direct toxic effect from the plant on oocytes, thereby making them unable to capture this protein, probably through interference with this capture factor.…”

Section: Discussionmentioning

confidence: 99%

Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach

Sousa

Rocha²,

Sabóia‐Morais

et al. 2013

Rev. Bras. Parasitol. Vet.

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Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus) microplus treated with Melia azedarach AbstractThis study aimed to analyze ovary histology and quantify total protein in the hemolymph of Rhipicephalus (Boophilus) microplus females treated with hexane extracts from green fruits of Melia azedarach. Eight engorged females were immersed in the extract at 0.25% concentration, and eight in water containing 5% acetone (control). The females were dissected 72 hours after treatment, and the ovaries were weighed and subjected to standard histological techniques. The total protein concentration was measured in the hemolymph of 200 females, of which 100 were treated as described above and 100 served as a control. In the treated group, ovary weight reduction and predominance of immature oocytes were observed. In addition, there were decreases in the diameters of the cytoplasm and germ vesicle of the oocytes in the treated group, compared with the controls. The protein concentration in the hemolymph was higher in the treated group than in the controls. The morphological changes observed in the treated ovaries included: presence of vacuolization; alteration of oocyte morphology, which changed from rounded to elongated; deformation of the chorion; and disorganization of the yolk granules. These results demonstrate the action of M. azedarach fruit extracts on R. (B.) microplus oogenesis.

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Effects of 20-hydroxyecdysone and other hormones on egg development, and identification of a vitellin-binding protein in the ovary of the tick, Amblyomma hebraeum

Cited by 37 publications

References 42 publications

Three Halloween genes from the Varroa mite,Varroa destructor(Anderson & Trueman) and their expression during reproduction

Three Halloween genes from the Varroa mite,Varroa destructor(Anderson & Trueman) and their expression during reproduction

Separation and detection of vitellogenin in fish plasma by capillary zone electrophoresis

Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach

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