Effects of 24-epibrassinolide on in vitro micropropagation of highbush blueberry

Abstract

In order to elucidate effects of 24 epibrassinolide (EB) on the efficiency of in vitro regeneration of highbush blueberry (Vaccinium corymbosum L., cv. Brigitta blue), we analyzed variability of seven biopro ductive parameters in regenerant plants cultured on nutrient media differing in hormonal composition. The additive action of EB, cytokinin (2iP), and auxin (IAA) was discovered for several parameters, such as repro duction coefficient, weight of regenerant plants, and the content of anthocyanin pigments. The greatest reproduction coefficients were noted in regenerant plants cultured on the medium containing 0.25 mg/L EB, 7.25 mg/L 2iP, and 1.00 mg/L IAA, as well as on the medium with 0.05 mg/L EB, 2.00 mg/L 2iP, and 0.50 mg/L IAA. It is suggested that low concentrations of 2iP and EB, applied in certain combinations, can be used for the industrial in vitro propagation of regenerant plants of highbush blueberry varieties.Abbreviations: 2iP-6 (γ,γ dimethylallylamino)purine; BSbrassinosteroids; EB-24 epibrassinolide; GA-genuine antho cyanins; LA-leucoanthocyanins; RC E -reproduction coeffi cient for explants; RC S -reproduction coefficient for shoots; TAP-total content of anthocyanin pigments; WPM-nutrient woody plant medium.

Effects of inorganic salts concentration and alternative plant growth regulators on the in vitro organogenesis of a new hybrid Cymbidium

“…Moreover, it was used to control hyperhydricity in in vitro carnation cultures (Lukatkin et al, 2017). 24-EpiB inhibited PLB formation in another hybrid Cymbidium cultivar (Teixeira da Silva, 2013b) but enhanced growth (improved rooting) in highbush blueberry (Kudryashova et al, 2012).…”

Possible replacement of cytokinins and auxins in culture in vitro

“…Виявилося, що додавання до складу живильного середовища препаратів цитедоф та дропп із цитокініновою активністю стимулює процес утворення адвентивних бруньок, формування мікростебел та збільшує коефіцієнт розмноження у бересклета карликового, діоскореї кавказьської та кірказона маньчжурського [3]. Інша модифікація полягала в тому, що за знижених норм використання ауксинів та цитокінінів до живильного МС-середовища у культурі in vitro додавали 0,25 мг/л епібрасиноліду для регенерації Vaccinium corymbosum [12].…”

The Role of Sequestrene 138 in Highbush Blueberry (Vaccinium corymbosum L.) Micropropagation

“…Research on highbush blueberry micropropagation was started in the 1980s (Reed and Abdelnour-Esquivel, 1991) and then broadened and accelerated in recent years to improve the existing micropropagation protocols (Gao et al, 2018;Hung et al, 2016;Kudryashova et al, 2012;Ru zi c et al, 2012). In the beginning, research was focused on finding the optimal culture media for micropropagation and checking the influence of diverse growth regulators (Chandler and Draper, 1986;Da Silva et al, 2006;Eccher and Noe, 1989;Eccher et al, 1986;Gajdo sov a et al, 2006;Isutsa et al, 1994;Ostroluck a et al, 2002Ostroluck a et al, , 2004Reed and Abdelnour-Esquivel, 1991;Tetsumura et al, 2008;Zhang et al, 2006).…”

“…The results showed that the most suitable medium for this species was McCown's Woody Plant Basal Salt Mixture, or Woody Plant Medium (WPM), as described by Lloyd and McCown (1980). In the following years, the influence of different plant hormones on the efficiency of in vitro regeneration of highbush blueberry was studied (Kudryashova et al, 2012), and other investigations also were made on the antioxidant and anti-inflammatory properties of V. corymbosum L. (Samad et al, 2014).…”

“…The latest scientific research was carried out to investigate the hyperhydricity-induced ultrastructural and physiological changes in blueberry (Gao et al, 2018), the effects of LED lights used for in vitro culture (Hung et al, 2016), the effect of 24-epibrassinolide on in vitro micropropagation of highbush blueberry (Kudryashova et al, 2012), and the use of a bioreactor in lowbush blueberry micropropagation (Debnath, 2009). Leaf pigment content and composition also provide important information about the physiological status of the in vitro plant.…”