Effects of 3 Endodontic Bioactive Cements on Osteogenic Differentiation in Mesenchymal Stem Cells

Lee, Bin-Na; Lee, Kkot-Nim; Koh, Jeong‐Tae; Min, Kyung-San; Chang, Hoon-Sang; Hwang, In-Nam; Hwang, Yun-Chan; Oh, Won-Mann

doi:10.1016/j.joen.2014.01.036

Cited by 72 publications

(71 citation statements)

References 42 publications

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“…Particularly, MTA has a wide range of possibilities in endodontic treatments [65] because of its clinical use involving the direct contact of this biomaterial with periradicular and pulpal tissues, contributing not only to cytotoxicity, genotoxicity, and proliferation but also to the differentiation of odontoblasts and osteoblasts [66, 67]. …”

Section: Discussionmentioning

confidence: 99%

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Victoria-Escandell

Ibáñez-Cabellos

Cutanda

et al. 2017

Stem Cells International

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Human dental pulp stem cells (HDPSCs) are of special relevance in future regenerative dental therapies. Characterizing cytotoxicity and genotoxicity produced by endodontic materials is required to evaluate the potential for regeneration of injured tissues in future strategies combining regenerative and root canal therapies. This study explores the cytotoxicity and genotoxicity mediated by oxidative stress of three endodontic materials that are widely used on HDPSCs: a mineral trioxide aggregate (MTA-Angelus white), an epoxy resin sealant (AH-Plus cement), and an MTA-based cement sealer (MTA-Fillapex). Cell viability and cell death rate were assessed by flow cytometry. Oxidative stress was measured by OxyBlot. Levels of antioxidant enzymes were evaluated by Western blot. Genotoxicity was studied by quantifying the expression levels of DNA damage sensors such as ATM and RAD53 genes and DNA damage repair sensors such as RAD51 and PARP-1. Results indicate that AH-Plus increased apoptosis, oxidative stress, and genotoxicity markers in HDPSCs. MTA-Fillapex was the most cytotoxic oxidative stress inductor and genotoxic material for HDPSCs at longer times in preincubated cell culture medium, and MTA-Angelus was less cytotoxic and genotoxic than AH-Plus and MTA-Fillapex at all times assayed.

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Section: Discussionmentioning

confidence: 99%

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Victoria-Escandell

Ibáñez-Cabellos

Cutanda

et al. 2017

Stem Cells International

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“…, Lee et al . ). In addition, MTA stimulates odontoblastic differentiation of dental pulp cells (Seo et al .…”

Section: Introductionmentioning

confidence: 97%

Effects of a novel light‐curable material on odontoblastic differentiation of human dental pulp cells

Lee

Chang

et al. 2016

Int Endodontic J

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“…The most widely used root-end filling material is a mineral trioxide aggregate (MTA), which is a calcium-silicate cement. MTA possesses several advantages, such as good sealing ability [1], acceptable mechanical properties [2], biocompatibility [3], and some antibacterial properties [4,5]. However, the main drawbacks of MTA are its high cost, low radiopacity, difficult manipulation and long setting time [2,6].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of selected properties of a new root repair cement containing surface pre-reacted glass ionomer fillers

et al. 2016

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Materials and methods:The antibacterial effect of S-PRG, MTA and IRM cements were tested against Porphyromonas gingivalis and Enterococcus faecalis after one and three-days of aging of the cements. Set cements were immersed in distilled water for 4 hours to 28 days and ion releasing ability was evaluated. Initial and final setting times of all cements were evaluated using Gilmore needles. The push-out bond strength between radicular dentin and all cements was tested at different levels of the roots.Results: S-PRG and IRM cements but not MTA cement demonstrated significant antibacterial effect against Porphyromonas gingivalis. All types of cements exhibited significant antibacterial effect against Enterococcus faecalis without being able to eliminate the bacterium. S-PRG cement provided continuous release of fluoride, strontium, boron, sodium, aluminum and zinc throughout all tested time points. Both initial and final setting times were significantly shorter for S-PRG and IRM cements in comparison to MTA. The push-out bond strength was significantly lower for S-PRG cement in comparison to MTA and IRM at coronal and middle levels of the roots. Conclusions:S-PRG cement demonstrated significant antibacterial effects against endodontic pathogens, multiple ion releasing ability, relatively short setting time and low bonding strength.Clinical relevance: S-PRG cement can be used as a one-visit root repair material with promising antibacterial properties and ion releasing capacity.

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Effects of 3 Endodontic Bioactive Cements on Osteogenic Differentiation in Mesenchymal Stem Cells

Cited by 72 publications

References 42 publications

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Effects of a novel light‐curable material on odontoblastic differentiation of human dental pulp cells

Evaluation of selected properties of a new root repair cement containing surface pre-reacted glass ionomer fillers

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