Effects of 4-nonylphenol and 17α-ethynylestradiol exposure in the Sydney rock oyster, Saccostrea glomerata: Vitellogenin induction and gonadal development

Andrew, M.N.; Dunstan, R. Hugh; O’Connor, Wayne A.; Zwieten, Lukas Van; Nixon, Brett; MacFarlane, Geoff R.

doi:10.1016/j.aquatox.2008.03.003

Cited by 74 publications

(24 citation statements)

References 44 publications

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“…\0.4-0.7% (Dinamani 1974;Cox et al 1996). Sex assignment was required due to the hypothesised differences in vitellogenin induction between males and females (Blaise et al 1999(Blaise et al , 2003, that vitellogenin induction in males is strong evidence for estrogen mediated endocrine disruption, and finally due to the established effects of estrogenic exposure on sex ratio (Andrew et al 2008). Each individual oyster was prepared for histological examination resulting in a total of 400 analyses.…”

Section: Histological Analysismentioning

confidence: 99%

“…The extraction (modified extraction from Gagné and Blaise 2000) and analysis of oyster gonadal samples were performed using protocols outlined in Andrew et al (2008). Briefly, 100 mg of oyster gonadal tissue was homogenized in 225 ll citrate buffer (pH 6.5 10 mM and with 16 mg/ml polyethylene glycol) and 25 ll of the protease inhibitor aprotinin then stored at -80°C.…”

Section: Experimental Designmentioning

confidence: 99%

“…The organic phase was then separated and dried under N 2 , then re-suspended in 1,000 ll of PBS. Analysis of vitellogenin was performed via HPLC analysis using conditions outlined in Andrew et al (2008), with the vitellogenin peak initially verified via proteomic sequencing (1DNanoLC ESI MS/MS). It should be acknowledged our use of the term vitellogenin implies the analyte is a vitellogenin-like protein based on genomic/ proteomic sequence similarity to known vitellogenin from other taxa (molluscs, fish spp.).…”

Section: Experimental Designmentioning

confidence: 99%

“…Significant increases in vitellogenin were also found in males exposed to 50 ng/l EE2. Further, exposure to NP (100 lg/l) and EE2 (50 ng/l) induced intersex gametal status for some individuals (Andrew et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster

et al. 2010

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Although mounting evidence suggests exposure to estrogenic contaminants increases vitellogenin production in molluscs, demonstration of dose-response relationships and knowledge of the temporal nature of the vitellogenin response with continual exposure is currently lacking for biomarker utility. To address this knowledge gap, adult Sydney rock oysters, Saccostrea glomerata, were exposed to a range of environmentally relevant concentrations of 17α-ethynylestradiol (EE2) (0, 6.25, 12.5, 25 or 50 ng/l) in seawater under laboratory conditions. Vitellogenin induction and gonadal development was assessed following 4, 21 and 49 days exposure to EE2. Vitellogenin was found to increase in a dose dependent manner with EE2 exposure for females (4 and 49 days) and males (4 and 21 days). Histological examination of gonads revealed a number of individuals exhibited intersex (ovotestis) in 50 ng/l EE2 (after 21 days) and in 6.25 and 12.5 ng/l EE2 (after 49 days). Furthermore, a significant shift towards females was observed following 49 days exposure at 50 ng/l EE2 suggesting estrogenic exposure is capable of facilitating a progression for protandric males from male-intersex-female gametal status. Increases in female vitellogenin (4 days) were predictive of later increases in female developmental stages at 21 days and increases in oocyte area following 49 days. Male vitellogenin (4 days) was predictive of decreased male percentages and lower male developmental stages at 49 days. Vitellogenin in S. glomerata is a predictive biomarker of estrogenic exposure and effect if sampled soon after exposure and at the commencement of a gonadal development cycle.

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Section: Histological Analysismentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster

et al. 2010

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“…It is commonly detected in soil, rivers, lakes, groundwater, sediment from aquifers, seawater and marine sediment in nanograms per liter (Yu et al, 2013). It was documented that EE2, even in low amounts, induced vitellogenesis in rainbow trout (Aerni et al, 2004), oysters (Andrew et al, 2008) and frogs (Hogan et al, 2008) suggesting its high endocrine disrupting potential.…”

Section: Introductionmentioning

confidence: 98%

Fungal transformation of 17α-ethinylestradiol in the presence of various concentrations of sodium chloride

Różalska

Bernat

Michnicki

et al. 2015

International Biodeterioration & Biodegradation

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Endocrine Disruption in Molluscs: Processes and Testing

McClellan‐Green

2013

Endocrine Disrupters

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Effects of 4-nonylphenol and 17α-ethynylestradiol exposure in the Sydney rock oyster, Saccostrea glomerata: Vitellogenin induction and gonadal development

Cited by 74 publications

References 44 publications

Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster

Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster

Fungal transformation of 17α-ethinylestradiol in the presence of various concentrations of sodium chloride

Endocrine Disruption in Molluscs: Processes and Testing

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