Effects of 4‐nonylphenol on fecundity and biomarkers of estrogenicity in fathead minnows (<i>Pimephales promelas</i>)

Giesy, John P.; Pierens, Susan L.; Snyder, Erin M.; Miles‐Richardson, Stephanie; Kramer, Vincent J.; Snyder, Shane A.; Nichols, Krista M.; Villeneuve, Daniel A.

doi:10.1002/etc.5620190520

Cited by 44 publications

(23 citation statements)

References 59 publications

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“…The GVBD inhibition was attributed to the estrogen agonistic action of 4-NP. The estrogenic property of 4-NP was also shown by Baek, Park, Lee & Kim (2003) in longchin goby Chasmichthys dolichognathus and by Giesy et al (2000) in fathead minnow Pimephales promelas.In catfish, low concentrations of 4-NP up to 10 ng/mL stimulated GVBD and the higher concentrations inhibited it. The species variation may be one factor for different responses to 4-NP in the GVBD assay.…”

Section: Discussionmentioning

confidence: 81%

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Gautama¹,

Chaube²,

Joy³

2018

AquaSt

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Alkylphenols are known endocrine-disrupting chemicals present in water bodies as contaminants, affecting aquatic life adversely. In this study, post vitellogenic follicles of the catfish Heteropneustes fossilis were in vitro exposed to 4-nonylphenol (4-NP) at concentrations of 0.1, 0.5, 1, 10, 100 and 200 ng/mL for 12 and 24 h. Germinal vesicle breakdown (GVBD), an indicator of final oocyte maturation (FOM), and the mortality of ovarian follicles were monitored. Levels of estradiol-17β (E2), testosterone (T), progestins; progesterone (P4), 17α-hydroxyprogesterone (17-P), 17α, 20β-dihydroxy-4-pregnen-3-one (17, 20β-DP), and cortisol (F) were measured by ELISA or HPLC. The exposure to lower concentrations of 4-NP (0.1, 0.5, 1 and 10 ng/mL) induced GVBD time dependently and the highest rate of induction was up to 25% in 0.5 ng/mL group. The higher concentrations decreased GVBD. 4-NP significantly increased the mortality rate of follicles in a dosedependent manner. Concentrations ≥10 ng/mL produced more than 60% mortality. 4-NP elicited biphasic effects on all hormones measured, except in the level of 17-P. The levels of T, E2, P4, 17, 20β-DP and F increased at the lower concentrations and maximum increase was at 0.1 ng/mL dose for E2, P4 and 17, 20β-DP, at 0.5 ng/mL dose for F and at 1 ng/mL dose for T. Then, they were declined with increasing 4-NP concentration. In contrast, 17-P level exhibited a continuous decline in a dose-dependent manner. From the results, it is inferred that the low concentrations of 4-NP stimulated GVBD. Increase in E2 level might have lowered the rate of GVBD, despite the high level of 17,20β-DP, maturation-inducing steroid, and F. The cytotoxicity infused by 4-NP at higher concentrations adversely affected GVBD and steroid secretion, causing follicle mortality.

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Section: Discussionmentioning

confidence: 81%

Untitled

Gautama¹,

Chaube²,

Joy³

2018

AquaSt

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“…Asterisks show significant differences from controls (PB0.05). minnow, Pimephales promelas, a freshwater multiplespawning species, NP increased the plasma E2 levels and decreased egg production, although NP decreased plasma vitellogenin levels at the beginning of the breeding season (Giesy et al 2000). The induction of vitellogenin is reported to be one of the most sensitive biomarkers for assessing estrogenic effects (Heppell et al 1995;Sumpter and Jobling 1995).…”

Section: Discussionmentioning

confidence: 99%

“…The induction of vitellogenin is reported to be one of the most sensitive biomarkers for assessing estrogenic effects (Heppell et al 1995;Sumpter and Jobling 1995). However, Giesy et al (2000) reported that the vitellogenin assay is inappropriate for multiple-spawning species such as the fathead minnow because plasma E2 levels remain high during the spawning period. In addition, they concluded that NP increased endogenous E2 levels and then inhibited egg production via an indirect mechanism and not a direct estrogen-agonistic mechanism.…”

Section: Discussionmentioning

confidence: 99%

Estrogenicity of 4-nonylphenol and diethylstilbestrol on in vitro oocyte maturation of the dusky tripletooth goby,Tridentiger obscurus

Hwang

Kim

et al. 2010

Animal Cells and Systems

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This study investigated the estrogenicity of 4-nonylphenol (NP) and diethylstilbestrol (DES) in vitro during oocyte maturation in the marine fish, Tridentiger obscurus, using steroid hormone assays and GVBD assay. Vitellogenic (0.53 mm diameter) and fully vitellogenic (0.75 mm diameter) oocytes were in vitro exposed to NP (0.045Á453.82 nM) and DES (0.037Á372.62 nM). In vitellogenic oocytes, 45.38 and 453.82 nM NP and 3.73Á372.62 nM DES increased the estradiol-17b (E2)/testosterone (T) ratio. In fully vitellogenic oocytes, 0.45, 45.38 and 453.82 nM NP and 3.73 nM DES increased E2/T. In the GVBD assay, 0.45 and 4.54 nM NP and 0.037, 3.73 and 37.26 nM DES inhibited GVBD. These results suggest that NP and DES have estrogen-agonistic effects in oocyte maturation in T. obscurus. In addition, NP and DES have different sensitivity according to the oocyte developmental stage, and the estrogenagonistic effects of DES were greater than were those of NP.

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“…Vitellogenin protein levels often increase in fish exposed to pulp and paper mill effluents [7] due to the activation of ERa by estrogen or estrogenic compounds or by androgenic substances that may be metabolized to have estrogenic effects [28,29]. Elevated VTG mRNA levels have been observed in juvenile whitefish (Coregonus lavaretus) caged downstream from bleached kraft mills [11].…”

Section: Expression Analysis Suggests That Effluents Contained Edcsmentioning

confidence: 99%

Pulp and paper mill effluents induce distinct gene expression changes linked to androgenic and estrogenic responses in the fathead minnow (Pimephales promelas)

Werner

Ouellet

Cheng

et al. 2009

Enviro Toxic and Chemistry

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Although effluent treatment systems within pulp and paper mills remove many toxicants and improve wastewater quality, there is a need to understand and quantify the effectiveness of the treatment process. At a combined news and kraft pulp and paper mill in northwestern Ontario, Canada, fathead minnow (FHM) reproduction and physiology were examined before, during, and after a short-term (6-d) exposure to 10% (v/v) untreated kraft mill effluent (UTK), 25% (v/v) secondary treated kraft mill effluent (TK), and 100% (v/v) combined mill outfall (CMO). Although UTK exposure significantly decreased egg production, neither TK nor CMO caused any reproductive changes. The expression of six genes responsive to endocrine-disrupting compounds, stress, or metals was then examined in livers of these fish using real-time polymerase chain reaction. In female FHMs, none of the three effluents induced significant expression changes in any genes investigated. By contrast, in males there were significant increases in the mRNA levels of androgen receptor, estrogen receptor (ER) beta, and cytochrome P4501A (CYP1A) upon UTK and TK exposure but no changes in ERalpha or vitellogenin (VTG) gene expression, whereas CMO exposure significantly increased the mRNA levels of ERalpha, VTG, and CYP1A. Together, these results suggest that kraft effluent before and after biological treatment contained compounds able to induce androgenic effects in FHMs, and that combination of kraft and newsmill effluents eliminated the androgenic compounds while inducing distinct and significant patterns of gene expression changes that were likely due to estrogenic compounds produced by the newsmill.

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Effects of 4‐nonylphenol on fecundity and biomarkers of estrogenicity in fathead minnows (Pimephales promelas)

Cited by 44 publications

References 59 publications

Untitled

Untitled

Estrogenicity of 4-nonylphenol and diethylstilbestrol on in vitro oocyte maturation of the dusky tripletooth goby,Tridentiger obscurus

Pulp and paper mill effluents induce distinct gene expression changes linked to androgenic and estrogenic responses in the fathead minnow (Pimephales promelas)

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