Effects of 50 Hz electromagnetic field exposure on apoptosis and differentiation in a neuroblastoma cell line

Pirozzoli, Maria Celeste; Marino, Carmela; Lovisolo, Giorgio A.; Laconi, C.; Mosiello, Lucia; Negroni, Anna

doi:10.1002/bem.10130

Cited by 71 publications

(50 citation statements)

References 29 publications

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“…Pirozzoli et al (2003) finding confirmed our results. They have not found alterations in the cellular ability to undergo programmed cell death, after exposure of the neuroblastoma cell line to 50 Hz electromagnetic field (Pirozzoli et al 2003).…”

Section: Dapi Staining Resultssupporting

confidence: 93%

“…Other researches also confirmed that EL-EMF cannot promote caspase activation and cannot trigger the apoptosis pathway (Akdag et al 2010) (Jiménez-García et al 2010). Pirozzoli et al (2003) finding confirmed our results. They have not found alterations in the cellular ability to undergo programmed cell death, after exposure of the neuroblastoma cell line to 50 Hz electromagnetic field (Pirozzoli et al 2003).…”

Section: Dapi Staining Resultssupporting

confidence: 88%

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Extremely low frequency electromagnetic field sensitizes cisplatin-resistant human ovarian adenocarcinoma cells via P53 activation

2015

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In the following study, extremely low frequency electromagnetic fields (EL-EMF) radiation was used to restore sensitivity in the cisplatin-resistant A2780 ovarian cancer cells. For this purpose A2780 cells were treated with different doses of cisplatin and EL-EMF (50 Hz, 200 gauss, and 2 h) alone. Cytotoxicity was the measurement using MTT assay. After calculating IC 50 for cisplatin (90 lg/ml) a lower concentration from IC 50 (30 and 60 lg/ml) was used to be combined with EL-EMF. We compare the effects of each cisplatin, EL-EMF and combination groups using acridine orange-propidium iodide (AO/PI) and DAPI staining, caspase 3/9 activation assay and Annexin/PI assay. We also assessed changes in P53 and Matrix metalloproteinases 2 (MMPs) gene expression with semi-quantitative RT-PCR. Results indicated an EL-EMF-dependent proliferative decrease which was found \10 %, and occurred independently of cisplatin. The decreased proliferation rate for 30 and 60 lg/ml cisplatin was about 20 and 40 %, respectively, while for synergistic groups 30 and 60 lg/ml cisplatin with 2 h EL-EMF exposer, showed 47 and 71 % decrease in viability in rats. DAPI staining indicated that chromatin break down significantly increased in synergistic groups. Acridine orange staining also confirmed MTT assay results. Caspase activity significantly increased in the combined groups. Semi-quantitative RT-PCR showed that in synergistic groups of cisplatin and EL-EMF, expression of P53 was increased but the expression level of MPP-2 gene decreased. Results from this study showed that changes generated by the non-invasive EL-EMF can make resistant cells sensitive to cisplatin.

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Section: Dapi Staining Resultssupporting

confidence: 93%

Section: Dapi Staining Resultssupporting

confidence: 88%

Extremely low frequency electromagnetic field sensitizes cisplatin-resistant human ovarian adenocarcinoma cells via P53 activation

2015

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“…Regarding differentiation under combined treatment, antagonistic interactions between ELF MF and differentiation promoters like NGF, MEL or retinoids, have been reported in different cell types [12,40,83,84]. The present results also show that the MF antagonizes the effects on AFP expression and ALB release, exerted by the assayed doses of MEL.…”

Section: Cellular Physiology and Biochemistry Cellular Physiology Andsupporting

confidence: 66%

Antagonistic Effects of a 50 Hz Magnetic Field and Melatonin in the Proliferation and Differentiation of Hepatocarcinoma Cells

Cid¹,

Úbeda²,

Hernández-Bule³

et al. 2012

Cell Physiol Biochem

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Background/Aims: Epidemiological and experimental evidence exists indicating that exposure to weak, extremely low frequency magnetic fields (ELF - MF) could affect cancer progression. It has been proposed that such hypothetical action could be mediated by MF-induced effects on the cellular response to melatonin (MEL), a potentially oncostatic neurohormone. The present study investigates the response of HepG2 cells to intermittent exposure to a 50 Hz, 10 µT MF, in the presence or absence of MEL at physiological (10 nM) or pharmacological doses (1 µM). Methods: The Trypan blue cell exclusion test, BrdU incorporation and PCNA expression assays were carried out to assess the cellular response in terms of viability and proliferation. In addition, albumin and alpha-fetoprotein, were analyzed as specific hepatocellular differentiation markers. Results: The results indicate that the MF exerts significant cytoproliferative and dedifferentiating effects that can be prevented by 10 nM MEL. Conversely, MEL exerts cytostatic and differentiating effects on HepG2 that are abolished by simultaneous exposure to MF. Conclusion: As a whole, these results support the hypothesis that ELF - MF and MEL exert opposite, mutually counteracting effects on cell proliferation and differentiation.

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“…These parameters include the field strength, the wave form, the exposure cycle and duration, the genetic characteristics of the cells (66), the presence of pharmacological drugs or endogenous hormones like melatonin (19,67) or the redox status of the system (68). Pirozzoli et al (69) reported a significant increase (10%; p≤0.05) in proliferation of the human neuroblastoma cell line LAN-5, after 7 days of continuous exposure to 50 Hz, 1 mT MF. In our experiments a much shorter (2 days) intermittent exposure to a weaker (0.1 mT) MF induced a similar increase in proliferation (15%, p≤0.001) in other neuroblastoma line.…”

Section: Discussionmentioning

confidence: 99%

Influence of a 50 Hz magnetic field and of all-trans‑retinol on the proliferation of human cancer cell lines

Trillo

2012

Int J Oncol

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Abstract. In vitro exposure to power frequency magnetic fields (MF) has been reported to influence cell proliferation and differentiation. However, the nature of the response of different human cancer cell types to these fields has not been sufficiently characterized. The present work investigates the response of two proliferating human cell lines of neuroblastoma (NB69) and hepatocarcinoma (HepG2) to a 42 h, intermittent treatment with a weak, 100 µT, 50 Hz MF, alone or in combination with 0.5 µM all-trans-retinol (ROL), a retinoid currently applied in oncostatic therapies. In each experimental replicate the cell samples were submitted to one of the following treatment combinations: MF+/ROL+, MF+/ ROL-, MF-/ROL+ or MF-/ROL-. The proliferative response was determined by cell counting (Trypan blue exclusion), BrdU incorporation and by spectrophotometric analysis of total protein and DNA content. The results show that when administered separately, the two treatments, MF and ROL, significantly enhanced cell proliferation in both cell lines. In NB69 simultaneous administration of MF and ROL induced an additive effect on cell proliferation, associated to increased DNA content. By contrast, in HepG2 the ROL-induced cell proliferation and increased protein content were partially blocked by simultaneous exposure to MF. Taken together, these data show that both agents, a weak MF and ROL at a low concentration, induce proliferative responses in the two assayed human cell lines. However, significant differences were observed between the responses of the two cellular species to the combined treatment with ROL and MF, indicating that the mechanisms underlying the cellular response to each of the two agents can mutually interact in a manner that is cell type-specific.

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Effects of 50 Hz electromagnetic field exposure on apoptosis and differentiation in a neuroblastoma cell line

Cited by 71 publications

References 29 publications

Extremely low frequency electromagnetic field sensitizes cisplatin-resistant human ovarian adenocarcinoma cells via P53 activation

Extremely low frequency electromagnetic field sensitizes cisplatin-resistant human ovarian adenocarcinoma cells via P53 activation

Antagonistic Effects of a 50 Hz Magnetic Field and Melatonin in the Proliferation and Differentiation of Hepatocarcinoma Cells

Influence of a 50 Hz magnetic field and of all-trans‑retinol on the proliferation of human cancer cell lines

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