Effects of 50 Hz rotating magnetic field on the viability ofEscherichia coliandStaphylococcus aureus

Abstract

This study presents results of research on the influence of rotating magnetic field (RMF) of the induction of 30 mT and the frequency of 50 Hz on the growth dynamics and cell metabolic activity of E. coli and S. aureus, depending on the exposure time. The studies showed that the RMF caused an increase in the growth and cell metabolic activity of all the analyzed bacterial strains, especially in the time interval t = 30 to 150 min. However, it was also found that the optical density and cell metabolic activity after exposition to RMF were significantly higher in S. aureus cultures. In turn, the study of growth dynamics, revealed a rapid and a significant decrease in these values from t = 90 min) in the case of E. coli samples. The obtained results prove that RMF (B = 30 mT, f = 50 Hz) has a stimulatory effect on the growth and metabolic activity of E. coli and S. aureus. Furthermore, taking into account the time of exposure, stronger influence of RMF on the viability was observed in S. aureus cultures, which may indicate that this effect depends on the shape of the exposed cells.

Modification of bacterial cellulose through exposure to the rotating magnetic field

“…In previous studies we proved that the exposure to the rotating magnetic field causes a significant increase (over 20%) of growth and metabolic activity (over 40%) of different microorganisms (Staphylococcus aureus, Escherichia coli, Serratia marcescens, Streptococcus mutans, Cronobacter sakazakii, Klebsiella oxytoca and Staphylococcus xylosus) compared to the bacteria nonexposed to RMF and incubated under standard (optimal) conditions (Fijałkowski et al, 2013;Fijałkowski et al, 2015a;Nawrotek, Fijałkowski, Struk, Rakoczy, & Kordas, 2014). The stimulatory influence of the exposure to RMF on the growth and cell metabolic activity can be attributed to the effects of RMF on bio-liquids.…”

Time Dependent Influence of Rotating Magnetic Field on Bacterial Cellulose

“…The effect of the magnetic stimulation on bacteria depends on the magnetic frequency, the magnetic induction, and the time of exposure. In previous studies we proved that the exposure to the RMF depended on those parameters and influenced growth and metabolic activity of the different microorganisms [39,40]. Biocellulose produced by G. xylinus contains more than 90% of water [55].…”

“…However, it should be noticed that the stimulating effect of the RMF on the functional parameters of microorganisms was shown to be dependent on the time of magnetic field exposition [39,40]. Similarly, the time of G. xylinus cultivation during which the BC biosynthesis occurs is considered as one of the crucial parameters affecting the properties of this material [22].…”

The role of cell hydration in realization of biological effects of non-ionizing radiation (NIR)

“…It is known that microbes are extra sensitive to the changes of environmental medium, including the variations of NIR in medium (Ahmed et al, 2013;Aslanimehr and Pahlevan, 2013;Ilyin et al, 2010a,b;Nawrotek et al, 2014;Segatore et al, 2012;Trushin, 2003). Because of this, they are widely used as one of the sensitive markers for environmental control (Gromozova et al, 2011).…”