Effects of a 50 Hz sinusoidal magnetic field on cell adhesion molecule expression in two human osteosarcoma cell lines (MG‐63 and Saos‐2)

Abstract

The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules (CAMs) in two human osteosarcoma cell lines (MG-63 and Saos-2) was investigated. In particular, the expression of two important integrins, VLA-2, the receptor for collagen, and VLA-5, the receptor for fibronectin, as well as CD44, were examined in both cell lines after these had been exposed for 7 and 14 days to a 50 Hz, 0.5 mT field. Cell surface morphology (scanning electron microscopy), cell growth characteristics (growth curves and cell cycle phase distribution), and cell death (necrosis and apoptosis) were also examined. The results demonstrate that no variations in surface morphology and cell death occurred between control and exposed cells in both MG-63 and Saos-2 cells, while significant changes were noted in cell growth and fibronectin and CD44 expression in MG-63 cells. The results are discussed in view of the important role that CAMs play in controlling various cancer cell functions, particularly proliferation and metastasis.

Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation

“…D), which indicates that the exterior magnetic field improved the cell proliferation on both the MHA and HA scaffolds, consistent with previous research. 2,33,34 The most significant cell proliferation was found on the MHA scaffold with the exterior magnetic fields, suggesting a likely synergistic effect between the MHA and the exterior magnetic fields. A similar trend is observed for the MC3T3-E1 cells in Figure 7.…”

“…The specimen was immersed into 100 mL of fluid and shaken ceaselessly at 37°C. A 2 mL aliquot of leaching solution was taken away at each time point (2,4,8, and 12 hours, and 1, 2, 3, and 5 days), while the same volume of fresh medium was simultaneously added back into the solution. The samples were further diluted eightfold for atomic absorption spectroscopy analysis to evaluate the iron content and calculate the masses of released magnetite.…”

“…Research has indicated that magnetic fields may stimulate the proliferation and differentiation of osteoblasts, promote the expression of growth factors such as bone morphological protein, increase osteointegration, and accelerate new bone formation. [1][2][3][4] Magnetic fields were also found to be beneficial in promoting the integration of bone and implants, increasing bone density and calcium content, and accelerating the healing of bone fractures. [5][6][7][8] Among the magnetic materials usually used in the biomedical field, magnetic nanoparticles (MNPs) have drawn great interest owing to their unique magnetic properties, including the fact that they become superparamagnetic at diameters of ,20 nm.…”

Beneficial Effects of Electromagnetic Radiation in Cancer

“…Moreover, the levels of expressed CD44 influence cell-cell interactions, cell adhesion and migration. According to Rudzki and Jothy (Rudzki & Jothy, 1997) and proposed mechanism of action by Santini, it is indicated that MF influence these molecules' expression, responsible for the transmission of vital signals, in the growth and metastasis of cancer (Santini et al, 2003).…”

P-selectin expression in a colon tumor model exposed by sinusoidal electromagnetic fields

“…The CAMs principally involved in these processes are those directed against important components of the extracellular matrix such as fibronectin, collagen, laminin, hyaluronan, heparan sulfate and elastin. Although a limited number of studies examining the effects of magnetic fields on cell adhesion are available, important insights into this issue have been previously provided (5).…”