Effects of a Ficoll-agarose system on early division and development of mesophyll protoplasts of winter oilseed rape (Brassica napus L.)

Millam, S.; Burns, Alan T. H.; Hocking, T. J.; Cattell, K. J.

doi:10.1007/bf00034370

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1991

1999

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“…Bienvenu were isolated according to our previous report [15] and filtered through a 64 #m mesh prior to being treated to one of the following methods…”

Section: Methodsmentioning

confidence: 99%

A comparative assessment of purification techniques for mesophyll protoplasts of Brassica napus L.

Millam

Burns

Hocking

1991

Plant Cell Tiss Organ Cult

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The effects of three different general purification protocols have been assessed quantitatively using mesophyll protoplasts of Brassica napus. Within the initial sample two distinct sub-populations were determined. The methods used influenced the ratio of the vacuolated to chloroplastic type protoplast sub-populations. Overall recovery rates of the initial sample varied according to the method used from 38% to 27%, but the relative recovery of the sub-populations varied considerably with a purified ratio of between 1.0:0.78 to 1.0:7.0. Size distribution profiles of the initial and purified populations are also presented.

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“…Bienvenu were isolated according to our previous report [15] and filtered through a 64 #m mesh prior to being treated to one of the following methods…”

Section: Methodsmentioning

confidence: 99%