Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

Abstract

PURPOSE: To verify the effect of highly concentrated platelet-rich plasma (hPRP) in the pathways of bone repair using non-critical defects in the calvaria of rabbits. METHODS: The hPRP was produced from collected venous blood of 21 rabbits. Four non-critical defects of 8 mm in diameter were created on the calvaria of each animal. The defects were all treated differently: autogenous particled bone (APB, group 1), autogenous particled bone associated with hPRP (APB + hPRP, group 2), isolated hPRP (group 3), and blood clot (control, group 4). Animals were submitted to euthanasia on the 2nd, 4th and 6th week postoperatively. Histological and histomorphometric analysis were carried through. RESULTS: After two weeks, groups 1 and 2 were in more advanced stage of repair than 3 and 4. At this period, comparing the groups 1 and 2, no significant differences were found between both, which also happened between the groups 3 and 4. However, after four and six weeks, the group 1 showed a more advanced stage of repair among all the other studied groups, while group 2 was in more advanced signs of bone repair than groups 3 and 4. Comparing groups 3 and 4, after four and six weeks, the least advanced stage of bone repair was found to be within group 3. CONCLUSION: The use of a highly concentrated PRP was considered prejudicial to the repair of non-critical defects in the calvaria of rabbits, either in the association of autogenous particled bone, when compared to autogenous particled bone alone, or in its isolated form, when compared to blood clot (control).

Leukocyte‐Platelet‐Rich Plasma (L‐PRP) Induces an Abnormal Histophenotype in Craniofacial Bone Repair Associated with Changes in the Immunopositivity of the Hematopoietic Clusters of Differentiation, Osteoproteins, and TGF‐β1

“…The fibrotic frame induced by L‐PRP was consistent with the findings of previous studies . Two different hypotheses should be taken into consideration when attempting to explain the results of this study.…”

“…The fibrotic frame induced by L-PRP was consistent with the findings of previous studies. 54,55 Two different hypotheses should be taken into consideration when attempting to explain the results of this study. The first hypothesis involves possible platelet-collagen interactions mimicking a thrombogenic-like effect, given that the activated platelets contain specific sites for different types of collagen interaction in its cellular membrane [56][57][58][59][60] and also express TGF-b1.…”

The use of bioactive factors to enhance bone regeneration: A narrative review

“…Conversely, when the potential of PRP was compared to positive controls, more heterogeneous results were reported. Several studies showed that, amongst different bone grafts, autologous bone is the gold standard for the regeneration of bony defects and adding PRP to autologous bone did not provide better bone regeneration (Aghaloo, Moy & Freymiller, ; Nagata, Messora et al., ; Oliveira Filho, Nassif, Malafaia, Ribas Filho & Ribas, ; Schlegel, Donath, Rupprecht, Falk & Zimmermann, ), with a positive effect demonstrated only during the early healing period (2–4 weeks; Nagata, Melo, et al., ; Wiltfang, Kloss, Kessler, Nkenke & Schultze‐Mosgau, ). It was also shown that interposing PRP between an autologous block graft and its bed did not confer significant benefits in terms of new bone formation (Miranda, Nary Filho, Padovan, Ribeiro & Nicolielo, ).…”

The roles of cellular and molecular components of a hematoma at early stage of bone healing

“…Oliveira Filho et al () surprisingly found that the addition of highly concentrated PRP to autograft reduces the graft capacity of bone healing. A blood clot alone even led to a more advanced stage of calvarial bone repair than PRP alone.…”