Effects of a Long-Term High-Fat Diet and Switching from a High-Fat to Low-Fat, Standard Diet on Hepatic Fat Accumulation in Sprague-Dawley Rats

Omagari, Katsuhisa; Kato, Shota; Tsuneyama, Koichi; Inohara, Chisato; Kuroda, Yu; Tsukuda, Hiroe; Fukazawa, Eri; Shiraishi, Kouya; Mune, Masatoshi

doi:10.1007/s10620-008-0303-1

Cited by 29 publications

(28 citation statements)

References 22 publications

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“…Previous studies have shown that normal rats fed with HFDs exhibit a bimodal pattern in body weight gain similar to that observed in humans (Omagari et al 2008, Powell et al 2014. But these studies apply long-term HFDs, usually for several months.…”

Section: Discussionmentioning

confidence: 92%

“…Loss of body weight significantly improves the ovulatory rate of PCOS women and ameliorates hyperandrogenism-related symptoms (Morris et al 2006, Tsagareli et al 2006, Panidis et al 2008. Though high-fat diets (HFDs) have been shown to cause obesity and infertility in female rodents (Omagari et al 2008, Powell et al 2014, how fat affects PCOS phenotypes is not fully elucidated. To gain a better understanding of interactions between hyperandrogenism and fat in PCOS, we applied HFDs with different fat contents (45% and 60%) in a rat model of PCOS induced by DHEA injection and observed the resultant changes of body weight, body fat content, menstrual cycles, ovarian morphology, serum hormone levels, glucose tolerance and several hormones and their receptor levels in the ovary and hypothalamus.…”

Section: Introductionmentioning

confidence: 99%

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High-fat diets exaggerate endocrine and metabolic phenotypes in a rat model of DHEA-induced PCOS

Zhang¹,

Yi²,

Zhang³

et al. 2016

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Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder with unclear etiology and unsatisfactory management. Effects of diets on the phenotype of PCOS were not fully understood. In the present study, we applied 45 and 60% high-fat diets (HFDs) on a rat model of PCOS induced by postnatal DHEA injection. We found that both DHEA and DHEACHFDs rats exhibited reproductive abnormalities, including hyperandrogenism, irregular cycles and polycystic ovaries. The addition of HFDs, especially 60% HFDs, exaggerated morphological changes of ovaries and a number of metabolic changes, including increased body weight and body fat content, impaired glucose tolerance and increased serum insulin levels. Results from qPCR showed that DHEA-induced increased expression of hypothalamic androgen receptor and LH receptor were reversed by the addition of 60% HFDs. In contrast, the ovarian expression of LH receptor and insulin receptor mRNA was upregulated only with the addition of 60% HFDs. These findings indicated that DHEA and DHEACHFDs might influence PCOS phenotypes through distinct mechanisms: DHEA affects the normal function of hypothalamus-pituitary-ovarian axis through LH, whereas the addition of HFDs exaggerated endocrine and metabolic dysfunction through ovarian responses to insulin-related mechanisms. We concluded that the addition of HFDs yielded distinct phenotypes of DHEA-induced PCOS and could be used for studies on both reproductive and metabolic features of the syndrome.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

High-fat diets exaggerate endocrine and metabolic phenotypes in a rat model of DHEA-induced PCOS

Zhang¹,

Yi²,

Zhang³

et al. 2016

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“…The lack of a statistically significant difference between high-fat diet and the control diet in biochemical and histopathological examination, suggest that oleic acid which has been in high fat diet content of soybean oil (13) and lard (14) would have protective effect (15). In another study, it was found that soybean oil decrease lipogenesis and liver fat density (16).…”

Section: Discussionmentioning

confidence: 99%

High Fatty Diet Effects on Rat Liver

et al. 2014

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“…Previous studies fed the Sprague-Dawley [47] rats and rabbits [48] with high-fat diets, during long periods, respectively 46 weeks and 24 weeks, and also did not detect any difference between the AST and ALT levels at any time of the experiment. Later, the comet assay was executed to measure the DNA damage in hepatocytes, although the highfat diets of lard were not able to harm the hepatocytes DNA, probably because the study lasted only four weeks.…”

Section: Discussionmentioning

confidence: 86%

Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

Leonardi-Carvalho¹,

Zucoloto²,

Ovídio³

et al. 2015

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Abstract:The study aim was to evaluate some effects of a high-fat diet and a high-protein-fat diet in the hepatic steatosis development in rats. Twenty nine Wistar rats were divided at random into three groups: Control: a control diet with soybean oil; HF: a high-fat diet with 50% of lard; HPF: a high-protein-fat diet with about 40% of protein and 50% of lard. The groups were fed with these diets during four weeks. The following parameters were analyzed: hepatic steatosis, serum and hepatic lipid profile, lipid peroxidation, antioxidants concentration and hepatocytes damage. The HF group showed the highest caloric intake per day and the highest weight gain (p<0.05). The hepatic cholesterol concentration was highest in the HF group and the serum total cholesterol concentration was highest in the HPF group (p<0.05). The macrovesicular steatosis was predominant in the HF group, with ballooning hepatocytes and Mallory bodies and in the HPF group predominant microvesicular steatosis was found, without ballooning hepatocytes and Mallory bodies. An increase in TBARS and a decrease in Vitamin E in the HF and HPF groups (p<0.05) was also found. The HF group showed the highest acid oleic deposit in the liver, followed by the HPF group. The SFA hepatic concentration was similar among the groups (p>0.05), whereas the MUFA concentration was higher in the HF and HPF (p>0.05) than the control group (p<0.05). Therefore, the experimental model used is an efficient model to study hepatic steatosis. The HF and HPF groups had the same behaviors for oxidative stress, serum glucose and hepatic damage in responses to the experimental diets, but these groups showed different esteatosis features.

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Effects of a Long-Term High-Fat Diet and Switching from a High-Fat to Low-Fat, Standard Diet on Hepatic Fat Accumulation in Sprague-Dawley Rats

Cited by 29 publications

References 22 publications

High-fat diets exaggerate endocrine and metabolic phenotypes in a rat model of DHEA-induced PCOS

High-fat diets exaggerate endocrine and metabolic phenotypes in a rat model of DHEA-induced PCOS

High Fatty Diet Effects on Rat Liver

Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

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