Effects of a Low-Protein Diet on Urinary Glycosaminoglycan Excretion in Adriamycin-Treated Rats

Benjelloun, A S; Merville, Pierre; Cambar, J.; Aparicio, Michel

doi:10.1159/000187321

Cited by 8 publications

(6 citation statements)

References 23 publications

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“…Our observations are in accord with the earlier study, in which increased excretion of glycosaminoglycans has been reported in rats with nephrotoxicity induced by adriamycin [40] and furthermore, high urinary excretion of glycosaminoglycans has been demonstrated as an early indicator of glomerular basement membrane damage in diabetic nephropathy [41]. It is well accepted that proteinuria also induces an increase in mesangial cell metabolism with increased synthesis and release of the extracellular matrix components in the urine [42]. Indeed, it has also been proposed that increased concentration of proteins in the urinary space might bring about chemotactic effects at the basolateral site.…”

Section: Discussionsupporting

confidence: 93%

Sulphated Polysaccharides: New Insight in the Prevention of Cyclosporine A‐Induced Glomerular Injury

Josephine

Veena

Amudha

et al. 2007

Basic Clin Pharma Tox

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Abstract:The scope of the current study was to examine the possible effects of sulphated polysaccharides against cyclosporine A-induced glomerular injury. Nephrotoxicity induced by cyclosporine A continues to be a major problem despite its potent immunosuppressive action. Adult male albino rats of Wistar strain were categorized into four groups. Two groups (II and IV) were administered cyclosporine A (25 mg/kg body weight, orally) for 21 days, in which Group IV rats were also treated simultaneously with sulphated polysaccharides (5 mg/kg body weight, subcutaneously) for the same period. A significant loss in body weight was noted in the cyclosporine A-induced rats. Renal damage was assessed in terms of decreased creatinine clearance and increased activity of lysosomal enzymes. The levels of glycoproteins were found to be decreased in the renal tissue, and a noticeable rise in glycosaminoglycanuria coupled with marked proteinuria was more prominent in the cyclosporine A-induced animals. Furthermore, the extent of kidney damage was assessed by histopathological findings. Toxic manifestations were also confirmed by transmission electron microscopic studies. These morphological abnormalities and other alterations in the renal tissue were significantly offset by sulphated polysaccharides supplementation. These findings underline that restoration of normal cells accredits sulphated polysaccharides, from Sargassum wightii , with nephroprotective role, against cyclosporine A-induced renal injury.

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Section: Discussionsupporting

confidence: 93%

Sulphated Polysaccharides: New Insight in the Prevention of Cyclosporine A‐Induced Glomerular Injury

Josephine

Veena

Amudha

et al. 2007

Basic Clin Pharma Tox

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“…In addition, curcumin might play a critical role in suppressing ROS mediated destruction of basement membrane and proteinuria (Raats et al ., 1997). In this respect, it is interesting to note that ADR has been demonstrated to cause increased urinary excretion of GAG and loss of glomerular GAG in rats (Benjelloun et al ., 1993). Fourth, the renoprotective effect of curcumin is also evident by a remarkable improvement of renal function, as judged by the restoration of GFR in ADR rats.…”

Section: Discussionmentioning

confidence: 99%

“…Urinary protein, creatinine and albumin, and plasma albumin were measured according to standard methods. Urine was also analysed for N‐acetyl‐β‐ D ‐glucosaminidase (NAG) (Moore & Morris, 1982), fibronectin (FN) (Rennard et al ., 1980) and glycosaminoglycan (GAG) (uronic acid content) (Benjelloun et al ., 1993). The glomerular filtration rate (GFR) was determined by insulin clearance according to standard methods.…”

Section: Methodsmentioning

confidence: 99%

Curcumin prevents adriamycin nephrotoxicity in rats

Venkatesan

Punithavathi

Arul

2000

British J Pharmacology

194

106

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The present study investigated the eect of curcumin on adriamycin (ADR) nephrosis in rats. The results indicate that ADR-induced kidney injury was remarkably prevented by treatment with curcumin. Treatment with curcumin markedly protected against ADR-induced proteinuria, albuminuria, hypoalbuminaemia and hyperlipidaemia. Similarly, curcumin inhibited ADR-induced increase in urinary excretion of N-acetyl-b-D-glucosaminidase (a marker of renal tubular injury), ®bronectin and glycosaminoglycan and plasma cholesterol. Curcumin restored renal function in ADR rats, as judged by the increase in GFR. The data also demonstrated that curcumin protected against ADR-induced renal injury by suppressing oxidative stress and increasing kidney glutathione content and glutathione peroxidase activity. In like manner, curcumin abolished ADR-stimulated kidney microsomal and mitochondrial lipid peroxidation. These data suggest that administration of curcumin is a promising approach in the treatment of nephrosis caused by ADR.

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“…In the first detailed description of ADR-NP [14], it was already reported that with the use of colloidal iron the glomerular polyanion disappeared quickly and was totally absent for at least 4 weeks. With polyethyleneimine as a probe, a 25% reduction in the number of heparan sulfate-associated sites was found in Ciclosporin in Adriamycin Nephropathy Nephron 1997;75:336-341 the lamina rara externa of rats with ADR-NP [24], and with cationic colloidal gold a 60% reduction of anionic sites in the GBM of rats with ADR-NP was observed [25], Benjclloun et al [26] found a reduced number of total glycosaminoglycans in the glomeruli and an increased uri nary excretion of both total glycosaminoglycans and of heparan sulfate. Since heparan sulfate is not only a deter minant for the charge-dependent permeability, but also for the size-dependent permeability [27], these alterations might explain the existing discrepancies with regard to the pathophysiology of proteinuria in ADR-NP.…”

Section: Discussionmentioning

confidence: 99%

Antiproteinuric Effect of Ciclosporin A in Adriamycin Nephropathy in Rats

Desassis

Raats

Bakker

et al. 1997

Nephron

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Ciclosporin A (CsA) can reduce proteinuria in various forms of human and experimental glomerulopathies. This antiproteinuric effect can be the result of a decrease of immunological damage, a decrease in the glomerular filtration rate (GFR), or a change in the permselective properties of the glomerular capillary wall. In this study we investigated the effect of CsA on Adriamycin-induced nephropathy in rats. A single intravenous injection of Adriamycin (5 mg/kg body weight) induced a severe nephrotic syndrome with a massive albuminuria ( ± 400 mg/24 h from 3 weeks onwards) and a hypoalbuminemia ( ± 7 mg/ml after 5 weeks). The IgG/albumin selectivity index was 0.16 ± 0.05, indicating a preferential loss of albumin. A 5-day treatment with CsA reduced the albumin excretion by almost 50% (from 336 ± 91 to 178 ± 58 mg/24 h; p = 0.002) and induced an increase in the serum albumin level (from 7.1 ± 4.1 to 12.8 ± 3.2 mg/ml; p = 0.002) in contrast to the vehicle olive oil (OO). CsA also decreased the GFR by 40% (from 0.74 ± 0.11 to 0.41 ± 0.11 mg/ml/100 g body weight; p = 0.002). Albuminuria corrected for the GFR (fractional excretion of albumin, FE_alb) was still significantly lower in CsA-treated than in OO-treated animals (FE_alb CsA: 1.35 ± 0.88, FE_albOO: 3.17 ± 2.29%; p = 0.0005). This suggests that other factors are also involved in the reduction of albuminuria. To exclude that CsA has an effect on the tubular reabsorption of albumin, we evaluated the blockade of the tubular reabsorption by lysine and found no difference in albuminuria between the CsA- and OO-treated groups. These experiments suggest that the antiproteinuric effect of CsA is not (only) due to a decrease in the GFR, but also to a decrease of the enhanced permeability of the glomerular capillary wall for albumin.

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Effects of a Low-Protein Diet on Urinary Glycosaminoglycan Excretion in Adriamycin-Treated Rats

Cited by 8 publications

References 23 publications

Sulphated Polysaccharides: New Insight in the Prevention of Cyclosporine A‐Induced Glomerular Injury

Sulphated Polysaccharides: New Insight in the Prevention of Cyclosporine A‐Induced Glomerular Injury

Curcumin prevents adriamycin nephrotoxicity in rats

Antiproteinuric Effect of Ciclosporin A in Adriamycin Nephropathy in Rats

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