Effects of a Metalloproteinase that Truncates P-selectin Glycoprotein Ligand on Neutrophil-induced Cardiac Dysfunction in Ischemia/Reperfusion

Lefer, Allan M.; Campbell, Barry; Shin, Yong-Kyoo

doi:10.1006/jmcc.1998.0828

Cited by 16 publications

(7 citation statements)

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“…Second, we excluded leukocytes and platelets in the perfusate, which might attenuate the magnitude of reperfusion injury. 27,28 Finally, ethidium was a potential carcinogen, and therefore, its application for diagnostic use in humans would be limited.…”

Section: Discussionmentioning

confidence: 98%

Optical mapping of myocardial reactive oxygen species production throughout the reperfusion of global ischemia

et al. 2006

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Reactive oxygen species (ROS) are short-lived, highly reactive chemical entities that play significant roles in all levels of biology. However, their measurement requires destructive preparation, thereby limiting the continuous measurement of ROS in a living tissue. We develop an optical mapping system to visualize ROS production in an isolated and perfused rat heart. By staining the heart with dihydroethidium (DHE), a 532-nm laser beam is directed to the epicardial surface, where we collect the red fluorescence (>600 nm) for semiquantitative analysis. With this system, ROS production as well as ventricular pressure and ECG in isolated perfused rat hearts are monitored throughout the reperfusion of global ischemia. Ischemia would decrease myocardial ROS production, while reperfusion would immediately result in sustained ROS overproduction. Optical mapping would provide information regarding the spatial distribution and temporal evolution of myocardial ROS production, which would enhance knowledge of the role of free radicals in cardiovascular biology.

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Section: Discussionmentioning

confidence: 98%

Optical mapping of myocardial reactive oxygen species production throughout the reperfusion of global ischemia

et al. 2006

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“…A metalloproteinase, mocarhagin, which cleaves PSGL-1, was introduced into the Langendorff apparatus to inhibit neutrophil interaction with the endothelium via PSGL-1. It was shown that mocarhagin was able to reduce neutrophil infiltration into the heart during reperfusion after an ischemic period [29].…”

Section: Selectin Ligands As a Therapeutic Targetmentioning

confidence: 99%

Targeting Adhesion Molecules in Cardiovascular Disorders

Haverslag¹,

Pasterkamp²,

Hoefer

2008

CHDDT

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Cell adhesion molecules are ubiquitously expressed proteins playing a central role in controlling cell migration, proliferation, survival, and apoptosis. Besides their key function in physiological maintenance of tissue integrity, adhesion molecules play an eminent role in various pathological processes. In cardiovascular disorders, cell adhesion molecules are particularly involved in atherogenesis and atherosclerotic plaque progression. They also play a critical role in myocardial infarction and reperfusion damage and a minor role in valvular stenosis and cardiomyopathy. Their common denominator: An increased expression of adhesion molecules involved in leukocyte extravasation and accumulation. Leukocyte extravasation is a multistep process, mediated by several cell adhesion molecules including selectins (P-, E- and L-), integrins and members of the Ig superfamily (ICAM-1, VCAM-1). These molecules can be targeted for imaging purposes (e.g. to identify atherosclerotic plaques) or can serve as biomarkers for plaque destabilization. Furthermore, cell adhesion molecules can serve as drugable targets to prevent leukocyte extravasation where warranted to decrease inflammatory tissue damage (e.g. reperfusion injury). Current techniques involve blocking of binding sites, targeted drug delivery using liposomes and polymeric particles as carriers or imaging of inflammation sites using labeled cells or antibodies. This review focuses on the role of cell adhesion molecules in cardiovascular disease and the use of targeting adhesion molecules for imaging purposes and local drug delivery in cardiovascular medicine.

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“…Platelet-related receptors and adhesion molecules, particularly glycoprotein IIb/IIIa and P-selectin, have been well described, and are reportedly activated in acute coronary syndromes [24, 25]. Soluble P-selectin is increased in patients with unstable angina [26], hypertension [27], after coronary spasm [28], and during AMI [29, 30, 31].…”

Section: Discussionmentioning

confidence: 99%

Soluble PECAM-1, but Not P-Selectin, Nor Osteonectin Identify Acute Myocardial Infarction in Patients Presenting with Chest Pain

et al. 1999

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We sought to determine plasma levels of platelet/endothelial cell adhesion molecule-1 (PECAM-1), P-selectin, and platelet-derived osteonectin, and prospectively compare these data with the discharge diagnosis in patients presenting with chest pain in a community hospital Emergency Department. Soluble antigens were measured by ELISA in 44 subjects including patients with acute myocardial infarction (AMI) (n = 13), chest pain of noncardiac origin (n = 17), and compared to those of age- and sex-matched healthy controls (n = 14). Elevated soluble PECAM-1 (64.5 ± 18.3 ng/ml, p = 0.019), but not P-selectin (149.5 ± 49.8 ng/ml, p = NS), nor osteonectin (549.5 ± 159.1 ng/ml, p = NS), occurred in the AMI group as compared to patients with noncardiac chest pain (46.2 ± 7.5 ng/ml, 118.2 ± 40.1 ng/ml, and 619.4 ± 74.4 ng/ml, respectively). Increased plasma PECAM-1 may serve as a useful marker in the early detection of patients with AMI. Larger studies will be necessary to confirm the utility of soluble PECAM-1 in identifying AMI among patients presenting with chest pain.

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Effects of a Metalloproteinase that Truncates P-selectin Glycoprotein Ligand on Neutrophil-induced Cardiac Dysfunction in Ischemia/Reperfusion

Cited by 16 publications

References 0 publications

Optical mapping of myocardial reactive oxygen species production throughout the reperfusion of global ischemia

Optical mapping of myocardial reactive oxygen species production throughout the reperfusion of global ischemia

Targeting Adhesion Molecules in Cardiovascular Disorders

Soluble PECAM-1, but Not P-Selectin, Nor Osteonectin Identify Acute Myocardial Infarction in Patients Presenting with Chest Pain

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