[Purpose] This study aimed to evaluate the effects of 12 weeks of non-face-to-face exercise intervention using mobile health (mHealth) on blood lipid levels and health-related physical fitness in obese women.[Methods] Thirty obese women (aged: 39.40 ± 11.07 years, percent body fat: 37.05 ± 5.15%) were enrolled, and all completed the study. Non-face-to-face exercises were performed for 12 weeks using a mHealth and smart tracker (Charge 4, Fitbit, USA). Participants were randomly assigned to an experimental (EXP) or control (CON) group. The 12-week exercise program using mHealth included resistance (twice a week for 60 min), aerobics (five times a week for 50 min), and flexibility (five times a week for 10 min).[Results] The results showed that high-density lipoprotein cholesterol (Post − Pre: 9.07 mg·dL<sup>-1</sup>, <i>p</i> < 0.001) and ratio of low-density to high-density lipoprotein cholesterol (Post − Pre: −0.71 mg·dL<sup>-1</sup>, <i>p</i> < 0.05) significantly changed during the intervention period in EXP. There were significant increases in sit-ups (Post − Pre: 7.73 numbers, <i>p</i> < 0.001), grip strength (Post − Pre: 2.92 kg, <i>p</i> < 0.001), and sit and reach (Post − Pre: 2.51 cm, <i>p</i> < 0.01) in EXP.[Conclusion] Non-face-to-face exercise using mHealth for 12 weeks improved blood lipid levels and health-related physical fitness; therefore, it can help improve compliance through self-monitoring and lifestyle changes by increasing physical activity.