Effects of a monocomponent protease on performance and protein utilization in 1- to 26-day-of-age turkey poults

Abstract

A study was conducted to evaluate effects of supplementation of a monocomponent protease on growth performance and protein utilization in Nicholas turkeys from 1 to 26 d of age fed corn-soy diets with reduced levels of CP and amino acids. Poults were placed into 40 batteries of 15 birds, and allocated to 5 treatments with 8 replicates each. A positive control diet [PC, 27.94% CP, 1.69 digestible Lys (DLys)] and a negative control diet (NC1; 24.21% CP, 1.46% DLys) were formulated. A second NC diet (NC2; 23.20% CP) was formulated with reduced CP and amino acids (AA) with expected improvements provided by a protease (1.014% CP, 0.024% DLys, 0.055% digestible TSAA, and 0.077% digestible Thr). The protease (75,000 PROT/g) was added at 200 mg/kg, at the expense of Celite (indigestible marker and filler) to create the NC1+P and NC2+P. Ileal contents were collected from all birds for apparent CP and AA digestibility determinations on d 26. A factorial arrangement of 2 × 2 + 1 was used (CP level × protease supplementation + PC). Turkeys fed the PC diet were 8.1% and 9.8% heavier (P < 0.05) compared with those fed the NC1 and NC2 diets and adding protease had no effect on BWG. Improvement in FCR (P < 0.05) were observed in birds fed the NC1 and NC2 diets. There was a main effect of protease (P < 0.0535) on FCR ratio, with birds fed diets with protease having better FCR than those without. There were no effects of the treatments on feed intake and carcass yield. Digestibility of CP was increased (P < 0.05) for turkeys fed the NC1+P diets in comparison to those fed the other diets, except the PC diet. Apparent digestibilities of Asp, Cys, Glu, Ile, Lys, Thr, and Val were affected by treatment, with birds fed the PC diet having the lowest digestibilities (P < 0.05). Poults fed protease-containing diets had better (main effect, P < 0.05) CP, Lys, and Cys digestibilities than those fed the diets without protease. Overall, the effects of the protease were limited but significant on FCR and specific AA digestibilities.

Effects of dietary coated protease on growth performance, feed utilization, nutrient apparent digestibility, intestinal and hepatopancreas structure in juvenile Gibel carp (Carassius auratus gibelio )

“…A specific enzyme, protease, was suggested adding to the feed efficiency, aimed to improve the protein utilization of the animals and reduce the required amount of dietary protein (and the nitrogen content) (Li et al, 2015;Oxenboll, Pontoppidan, & Fru-Nji, 2011). But it would also be expected to reduce the total content of nitrogen in manure helping to reduce nutrient excretions (Fru-Nji, Kluenter, Morten, & Pontoppidan, 2011;Vieira et al, 2013). The application of protease to improve protein digestibility has been studied extensively in poultry (Ghazi, Rooke, & Galbraith, 2003;Ghazi, Rooke, Galbraith, & Bedford, 2002;Marsman et al, 1997;Rubio, Brenes, & Centeno, 2003;Vieira et al, 2013).…”

“…But it would also be expected to reduce the total content of nitrogen in manure helping to reduce nutrient excretions (Fru-Nji, Kluenter, Morten, & Pontoppidan, 2011;Vieira et al, 2013). The application of protease to improve protein digestibility has been studied extensively in poultry (Ghazi, Rooke, & Galbraith, 2003;Ghazi, Rooke, Galbraith, & Bedford, 2002;Marsman et al, 1997;Rubio, Brenes, & Centeno, 2003;Vieira et al, 2013). It has also been reported on rainbow trout (Oncorhynchus mykiss) (Drew et al, 2005) and tilapia (Oreochromis niloticus × O. aureus) (Li et al, 2015).…”

Influence of exogenous acid protease in broiler chickens fed corn–soybean meal‐based diets

“…Zanella et al (1999) (Dosković et al, 2013). Poultry fed proteasecontaining diets had positive effect on CP, lysine and cystine digestibility than those fed the diets without protease (Vieira et al, 2013). In addition, a beneficial effect was found on the ileal digestibility of AAs when the chicks fed acid protease supplementation.…”

“…Additionally, there was a main effect of protease on FCR with birds fed diets with protease having a lower FCR than those without(Vieira et al, 2013;walk et al, 2018) Purbojo, LUIS, E. S., Tambunan R. D., and Pamungkas, D. (2004). Additionally, there was a main effect of protease on FCR with birds fed diets with protease having a lower FCR than those without(Vieira et al, 2013;walk et al, 2018) Purbojo, LUIS, E. S., Tambunan R. D., and Pamungkas, D. (2004).…”

The effects of Papain Crude Extract Addition in Diets on Broilers Production Performances

“…Furthermore the significance of interaction of papain crude extract and feed forms for feed conversion ratio (FCR) throughout the observation affirmed the role of crude papain application levels up to 0.07% in diets. Several previous researchers observed the role of supplementation of protease in the diet (Vieira et al, 2013) resulted a better feed intake (Mahejabin et al, 2015), body weight and FCR values to the chicken (Kame et al, 2015).This condition facilitated digestibility of amino acids in the diets to get a better FCR of broilers (Mejia et al, 2011). Similar response was reported by Yadav and Sah (2006)the use of 0.07% and 0.1 % acid protease in diets improved the feed efficiency of layer chicken, while Rada et al(2013)was divergent showed that the exogenous mono-component protease added into low protein broiler diet had no significant effect on both observed growth parameters carcass characteristic.…”