Effects of a New Allograft Processing Procedure on Graft Healing in a Canine Model: A Preliminary Study

Abstract

Graft healing in vivo can be affected by allograft processing. We asked whether a new processing technique influenced graft-host healing compared with autograft and a standard processing technique in a canine ulna model. We used bilateral intercalary allografts or autografts in the ulna of 13 skeletally mature male coonhounds. Each animal received two allografts, either one autograft and one allograft, or two autografts. At term (90 days), the graft sites were harvested. We assessed union with high-resolution xray imaging. Each specimen was processed for nondecalcified histologic analysis to assess the graft-host interface. Quantitative histomorphometric analysis was performed to determine spatial location and area of bone. Radiographic analysis, histologic analysis, and histomorphometric measures revealed no differences in union, mean total bone area, or total endosteal/intramedullary bone for the new process, standard process, and autografts. Our preliminary data suggest the new processing techniques may increase the safety of allograft transplantation without adversely affecting union when compared with standard processing techniques and autograft in a canine model.

Bone Allografts In Dentistry: A Review

“…This is balanced against disinfecting activity of the compound. According to Holzclaw et al, one hour exposure to H 2 0 2 does not have a profound effect on osteoinduction by bone allografts [60].…”

“…Bleaching is usually achieved by bathing allografts in a solution of hydrogen peroxide [H 2 0 2 ]. Exposure to H 2 0 2 diminishes or abolishes osteoinductivity [44], but the effect is time-dependent [59,60]. This is balanced against disinfecting activity of the compound.…”

Application of Bone Substitutes and Its Future Prospective in Regenerative Medicine

“…It was also established that chilling and boiling of bone tissues before using them as allografts led to destruction of their endogenous proteins and other factors resulting in poor and slow recovery [24]. The necessities posed by the devastation of World War II resulted in advancement and growth in the bone banking approach, with refinement of methods like autoclaving, freeze-drying, irradiating, demineralizing, and chemical treatment of the bone [25]. The advancement made during the war period continued with focus on civilian need and further refinement of the banking approach [23].…”

Nature-Inspired Nanotechnology and Smart Magnetic Activation: Two Groundbreaking Approaches Toward a New Generation of Biomaterials for Hard Tissue Regeneration

“…Today the use of grafts to assist the regeneration of long segmental bones is considered as a promising approach, with different alternatives including autologous vascularized bone grafts, homologous bone graft, heterologous bone graft (xenograft), or prostheses, each one of them dealing with both specific advantages and drawbacks, such as: donor site morbidity and limited available amount, possible immune response and viral transmission, possible animalderived pathogen transmission and risk of immunogenic rejection, high invasiveness and surgery-related systemic risks, long recovery time and need of prostheses revision [23][24][25][26][27].…”