Effects of a novel recombinant Gonadotropin-Releasing Hormone-1 vaccine on the reproductive function of mixed-breed dogs (Canis familiaris) in Taiwan

Chang, Ai-Mei; Chen, Chen‐Chih; Lee, Jai-Wei; Hou, Ding-Liang; Huang, Hsiao-Hui; Ke, Guan-Ming

doi:10.1016/j.vaccine.2023.02.061

Vaccine

2023

DOI: 10.1016/j.vaccine.2023.02.061

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Effects of a novel recombinant Gonadotropin-Releasing Hormone-1 vaccine on the reproductive function of mixed-breed dogs (Canis familiaris) in Taiwan

Ai-Mei Chang

Chen‐Chih Chen

Jai-Wei Lee

et al.

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Cited by 4 publications

References 33 publications

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Structure-guided design and evaluation of CRM197-scaffolded vaccine targeting GnRH for animal immunocastration

Duan,

Tang,

Liu

et al. 2024

Appl Microbiol Biotechnol

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Immunocastration is a humane alternative to surgical castration for controlling population and estrous behaviors in animals. Gonadotropin-releasing hormone (GnRH), the pivotal initiating hormone of the hormonal cascade in mammals, is the optimal target for immunocastration vaccine development. Cognate antigen-mediated cross-linking of B cell receptors (BCRs) is a strong activation signal for B cells and is facilitated by repetitive surface organizations of antigens. In this study, we describe the structure-guided design of highly immunogenic chimeric proteins with variant numbers of GnRH peptide insertion by epitope grafting. Linear B-cell epitopes of cross-reacting material 197 (CRM197) were replaced with multiple copies of GnRH peptide, and the inserts were displayed on the surface of the designs while maintaining the overall folding of CRM197. Among the seven designs, TCG13, which carries 13 copies of GnRH peptide, was the most immunogenic, and its immunocastration efficacy was evaluated in male mice. Vaccination with the BFA03-adjuvanted TCG13 induced potent humoral immunity and reduced the serum testosterone concentration in mice. It could significantly decrease sperm quality and severely impair gonadal function and fertility. These results demonstrate that CRM197 holds great value as a scaffold for epitope presentation in peptide-based vaccine development and supports TCG13 as a promising vaccine candidate for animal immunocastration. Key points • Provide a feasible way to design chimeric immunogen targeting GnRH by epitope grafting. • CRM197 can accommodate the insertion of multiple copies of heterologous epitope peptides. • Administration with the most immunogenic design led to effective immunocastration in male mice. Supplementary Information The online version contains supplementary material available at 10.1007/s00253-024-13348-3.

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Structure-guided design and evaluation of CRM197-scaffolded vaccine targeting GnRH for animal immunocastration

Duan,

Tang,

Liu

et al. 2024

Appl Microbiol Biotechnol

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The spatio-temporal distribution of aromatase cytochrome in ovary throughout the canine oestrous cycle

Lindh,

Kowalewski,

Goericke-Pesch

et al. 2024

Reprod. Fertil. Dev.

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Context New animal welfare legislation and ethical guidelines encourage alternative approaches for canine contraception, instead of surgical gonadectomy which is considered invasive and unjustified in healthy dogs. Aims Reversible contraception might be achieved by inhibition of aromatase (CYP19), an enzyme catalysing the conversion of androgens to oestrogens. This study provides insights into the spatio-temporal expression and distribution of aromatase in canine ovarian tissue. Methods Ovarian tissue was collected from 39 healthy and sexually mature bitches during different stages of the oestrous cycle: pro-oestrus (n = 8), oestrus (n = 12), dioestrus (n = 9) (luteal phase) and anoestrus (n = 10). Localisation of cytochrome P450 aromatase was determined by immunohistochemistry. Key results Aromatase activity in the dog is high during pro-oestrus, ovulation and early dioestrus. Comparing types of follicles and corpora lutea, the highest aromatase abundance was found in antral follicles and luteinising follicles, whereas corpora lutea and early antral follicles showed an intermediate presence of the enzyme. Interesting was the high abundance of aromatase in luteinising theca interna cells, prevailing over granulosa cells. Conclusions and implications Understanding of cells involved in oestradiol production is important for targeted inhibition of oestradiol synthesis, possibly offering an approach for contraception and suppression of oestrus.

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GnRH-immunocastration: an alternative method for male animal surgical castration

Wang,

Yang,

Zeng

et al. 2023

Front. Vet. Sci.

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Castration of male animals is intended to produce high-enhance quality of animal meat, prevent unpleasant taste, reduce aggressive behavior, and manage overbreeding. Over the years, Tranditional methods of mechanical and surgical castration have been employed over the years, but they fall short of meeting animal welfare requirements due to the associated risk of infection, pain, and stress. Immunocastration, specifically Gonadotropin-releasing hormone (GnRH)-immunocastration, targeting the hypothalamic–pituitary-testis (HPT) axis, has emerged as an animal-friendly alternative to surgical castration, effectively addressing these issues. This review seeks to systematically summarize the principles, development, current applications and challenges of GnRH-immunocastration, offering insights into its role in promoting animal welfare.

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Effects of a novel recombinant Gonadotropin-Releasing Hormone-1 vaccine on the reproductive function of mixed-breed dogs (Canis familiaris) in Taiwan

Cited by 4 publications

References 33 publications

Structure-guided design and evaluation of CRM197-scaffolded vaccine targeting GnRH for animal immunocastration

Structure-guided design and evaluation of CRM197-scaffolded vaccine targeting GnRH for animal immunocastration

The spatio-temporal distribution of aromatase cytochrome in ovary throughout the canine oestrous cycle

GnRH-immunocastration: an alternative method for male animal surgical castration

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