Effects of a Protein-Corona on the Cellular Uptake of Ferroferric Oxide Nanoparticles

Guo, Lingling; Feng, Zhang‐Qi; Liang, Cai; Zhang, Xi; Mou, Xianbo; Wang, Ting; He, Nongyue

doi:10.1166/jnn.2016.11361

Cited by 11 publications

(7 citation statements)

References 32 publications

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“… Proper uptake by cells Usually, proteins and polymer nanoparticles are removed by the cell by different mechanisms, for example, one of the effective factors mentioned in the anticancer drug Abraxane is the uptake of albumin nanoparticles by vascular endothelial cells [ 80 – 82 ]. Targeting The structure and sequence of the protein and the presence of numerous different functional groups allow the binding of the drug to specific sites in the protein and the binding of different targeting ligands to the protein Nano carrier [ 83 , 84 ]. …”

Section: Advantages and Disadvantaged Of The General Protein-based Nanoparticle Fabrication Methodsmentioning

confidence: 99%

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Protein nanoparticles in drug delivery: animal protein, plant proteins and protein cages, albumin nanoparticles

Kianfar¹

2021

J Nanobiotechnol

282

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In this article, we will describe the properties of albumin and its biological functions, types of sources that can be used to produce albumin nanoparticles, methods of producing albumin nanoparticles, its therapeutic applications and the importance of albumin nanoparticles in the production of pharmaceutical formulations. In view of the increasing use of Abraxane and its approval for use in the treatment of several types of cancer and during the final stages of clinical trials for other cancers, to evaluate it and compare its effectiveness with conventional non formulations of chemotherapy Paclitaxel is paid. In this article, we will examine the role and importance of animal proteins in Nano medicine and the various benefits of these biomolecules for the preparation of drug delivery carriers and the characteristics of plant protein Nano carriers and protein Nano cages and their potentials in diagnosis and treatment. Finally, the advantages and disadvantages of protein nanoparticles are mentioned, as well as the methods of production of albumin nanoparticles, its therapeutic applications and the importance of albumin nanoparticles in the production of pharmaceutical formulations.

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Section: Advantages and Disadvantaged Of The General Protein-based Nanoparticle Fabrication Methodsmentioning

confidence: 99%

“…The structure and sequence of the protein and the presence of numerous different functional groups allow the binding of the drug to specific sites in the protein and the binding of different targeting ligands to the protein Nano carrier [ 83 , 84 ].…”

Section: Advantages and Disadvantaged Of The General Protein-based Nanoparticle Fabrication Methodsmentioning

confidence: 99%

Protein nanoparticles in drug delivery: animal protein, plant proteins and protein cages, albumin nanoparticles

Kianfar¹

2021

J Nanobiotechnol

282

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“…It contributes to the effective loading of therapeutics in NPs in cancer cells. By contrast, the formation of fetal bovine serum (FBS) on the surface of ferroferric oxide NPs can minimize the nonspecific interactions between NPs and cells via lowering the NPs' surface energy and inducing a lower cellular uptake efficiency in the NPs [115]. The cellular uptake of NPs on immune cells is decreased in the presence of a recombinant fusion protein [116] or immunoglobulin corona [117], which confers stealth properties on the NPs for evading immune cells and reduces clearance by macrophages.…”

Section: Regulating Cellular Uptake and Improving Drug Deliverymentioning

confidence: 99%

“…lower cellular uptake efficiency in the NPs [115]. The cellular uptake of NPs on immune cells is decreased in the presence of a recombinant fusion protein [116] or immunoglobulin corona [117], which confers stealth properties on the NPs for evading immune cells and reduces clearance by macrophages.…”

Section: Regulating Cellular Uptake and Improving Drug Deliverymentioning

confidence: 99%

Insights into Characterization Methods and Biomedical Applications of Nanoparticle–Protein Corona

Lee

2020

Materials

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Nanoparticles (NPs) exposed to a biological milieu will strongly interact with proteins, forming “coronas” on the surfaces of the NPs. The protein coronas (PCs) affect the properties of the NPs and provide a new biological identity to the particles in the biological environment. The characterization of NP-PC complexes has attracted enormous research attention, owing to the crucial effects of the properties of an NP-PC on its interactions with living systems, as well as the diverse applications of NP-PC complexes. The analysis of NP-PC complexes without a well-considered approach will inevitably lead to misunderstandings and inappropriate applications of NPs. This review introduces methods for the characterization of NP-PC complexes and investigates their recent applications in biomedicine. Furthermore, the review evaluates these characterization methods based on comprehensive critical views and provides future perspectives regarding the applications of NP-PC complexes.

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“…It is thought that modulation of NP size and surface chemistry can proportionally affect the long-range physical field, which directly interacts with its surrounding media. , Noteworthy, manipulation of the NP surface by synthetic or natural agents with different molecular weights, sizes, and densities can affect the content and amount of proteins adsorbed to the NP surface along with types of biological fluids. − The most common strategy to analyze the protein–NP interaction and influence of PC on NP cellular uptake and cytotoxicity is to incubate them with different biological fluids. Among various biofluids, human serum and plasma, mouse serum and plasma, or fetal bovine serum (FBS), are commonly used in different in vitro and in vivo settings. For cell culture purposes, human serum and FBS have been frequently employed.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Effect of Breast Cancer Patients’ Plasma on the Targeting Ability of Folic Acid-Modified Chitosan Nanoparticles

et al. 2021

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The formation of protein corona (PC) around nanoparticles (NPs) has been reported inside biological conditions. This effect can alter delivery capacity toward the targeted tissues. Here, we synthesized folic acid-modified chitosan NPs (FA-CS NPs) using different concentrations of folic acid (5, 10, and 20%). FA-CS NPs were exposed to plasmas of breast cancer patients and healthy donors to evaluate the possibility of PC formation. We also monitored uptake efficiency in in vitro conditions after incubation with human breast cancer cell line MDA-MB-231 and monocyte/macrophage-like Raw264.7 cells. Data showed that the formation of PC around FA-CS NPs can change physicochemical properties coincided with the rise in NP size and negative surface charge. SDS-PAGE electrophoresis revealed differences in the type and content rate of plasma proteins attached to NP surface in a personalized manner. Based on MTT data, the formation of PC around NPs did not exert cytotoxic effects on MDA-MB-231 cells while this phenomenon reduced uptake rate. Fluorescence imaging and flow cytometry analyses revealed reduced cellular internalization rate in NPs exposed to patients' plasma compared to the control group. In contrast to breast MDA-MB-231 cells, Raw264.7 cells efficiently adsorbed the bare and PC-coated NPs from both sources, indicating the involvement of ligand−receptor-dependent and independent cellular engulfment. These data showed that the PC formed on the FA-CS NPs is entirely different in breast cancer patients and healthy counterparts. PC derived from patients' plasma almost abolishes the targeting efficiency of FA-CS NPs even in different mechanisms, while this behavior was not shown in the control group. Surprisingly, Raw264.7 cells strongly adsorbed the PC-coated NPs, especially when these particles were in the presence of patients' sera. It is strongly suggested that the formation of PC around can affect delivering capacity of FA-CS NPs to cancer cells. It seems that the PC-coated FA-CS NPs can be used as an efficient delivery strategy for the transfer of specific biomolecules in immune system disorders.

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Effects of a Protein-Corona on the Cellular Uptake of Ferroferric Oxide Nanoparticles

Cited by 11 publications

References 32 publications

Protein nanoparticles in drug delivery: animal protein, plant proteins and protein cages, albumin nanoparticles

Protein nanoparticles in drug delivery: animal protein, plant proteins and protein cages, albumin nanoparticles

Insights into Characterization Methods and Biomedical Applications of Nanoparticle–Protein Corona

Unraveling the Effect of Breast Cancer Patients’ Plasma on the Targeting Ability of Folic Acid-Modified Chitosan Nanoparticles

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