Effects of a specially pulsed electric field on an animal model of wound healing

Çınar, Kenan; Çömlekçi, Selçuk; Şenol, Nurgül

doi:10.1007/s10103-008-0631-6

Cited by 20 publications

(11 citation statements)

References 17 publications

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“…In light of this, our study demonstrated for the first time that ES-exposed skin fibroblasts were able to reduce the size of a 3D collagen matrix. These findings concur with those of an animal study showing that direct current EF favorably affected collagen synthesis and wound closure/contraction [62]. Wound contraction is predominantly myofibroblast-dependent [63].…”

Section: Discussionsupporting

confidence: 91%

Electrical Stimulation Promotes Wound Healing by Enhancing Dermal Fibroblast Activity and Promoting Myofibroblast Transdifferentiation

et al. 2013

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Electrical stimulation (ES) has long been used as an alternative clinical treatment and an effective approach to modulate cellular behaviours. In this work we investigated the effects of ES on human skin fibroblast activity, myofibroblast transdifferentiation and the consequence on wound healing. Normal human fibroblasts were seeded on heparin-bioactivated PPy/PLLA conductive membranes, cultured for 24 h, and then exposed to ES of 50 or 200 mV/mm for 2, 4, or 6 h. Following ES, the cells were either subjected to various analyses or re-seeded to investigate their healing capacity. Our findings show that ES had no cytotoxic effect on the fibroblasts, as demonstrated by the similar LDH activity levels in the ES-exposed and non-exposed cultures, and by the comparable cell viability under both conditions. Furthermore, the number of viable fibroblasts was higher following exposure to 6 h of ES than in the non-exposed culture. This enhanced cell growth was likely due to the ES up-regulated secretion of FGF-1 and FGF-2. In an in vitro scratch-wound assay where cell monolayer was used as a healing model, the electrically stimulated dermal fibroblasts migrated faster following exposure to ES and recorded a high contractile behaviour toward the collagen gel matrix. This enhanced contraction was supported by the high level of α-smooth muscle actin expressed by the fibroblasts following exposure to ES, indicating the characteristics of myofibroblasts. Remarkably, the modulation of fibroblast growth continued long after ES. In conclusion, this work demonstrates for the first time that exposure to ES promoted skin fibroblast growth and migration, increased growth factor secretion, and promoted fibroblast to myofibroblast transdifferentiation, thus promoting wound healing.

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Section: Discussionsupporting

confidence: 91%

Electrical Stimulation Promotes Wound Healing by Enhancing Dermal Fibroblast Activity and Promoting Myofibroblast Transdifferentiation

et al. 2013

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“…Formation and deposition of collagen fibers are particularly important for wound repair because collagen is necessary for granulation tissue organization and tissue membrane regeneration during the wound healing process [ 23 ]. Additionally, more collagen observed in Group SC showed that electrical stimulation generated by the OPP may have affected collagen synthesis by the fibroblasts, as observed in previous study [ 24 ]. These results suggest that OPP-derived electrical stimulation treatment can expedite collagen synthesis in the wound lesion and better regenerate the basal and epidermis layer to reduce the wound defect area.…”

Section: Discussionsupporting

confidence: 79%

A Disposable Photovoltaic Patch Controlling Cellular Microenvironment for Wound Healing

Jang

Jeong

et al. 2018

IJMS

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Electrical stimulation (ES) is known to affect the wound healing process by modulating skin cell behaviors. However, the conventional clinical devices that can generate ES for promoting wound healing require patient hospitalization due to large-scale of the extracorporeal devices. Herein, we introduce a disposable photovoltaic patch that can be applied to skin wound sites to control cellular microenvironment for promoting wound healing by generating ES. In vitro experiment results show that exogenous ES could enhance cell migration, proliferation, expression of extracellular matrix proteins, and myoblast differentiation of fibroblasts which are critical for wound healing. Our disposable photovoltaic patches were attached to the back of skin wound induced mice. Our patch successfully provided ES, generated by photovoltaic energy harvested from the organic solar cell under visible light illumination. In vivo experiment results show that the patch promoted cutaneous wound healing via enhanced host-inductive cell proliferation, cytokine secretion, and protein synthesis which is critical for wound healing process. Unlike the current treatments for wound healing that engage passive healing processes and often are unsuccessful, our wearable photovoltaic patch can stimulate regenerative activities of endogenous cells and actively contribute to the wound healing processes.

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“…The pyroelectric and piezoelectric nature of the epidermal layer of skin may help to find novel ways to manipulate skin tissue adaptation and remodeling (Athenstaedt et al 1982 ). Guided cell movement and migration can be achieved by applying small electric fields, and consequently can improve in vivo skin healing (Cinar et al 2009 ). Such studies demonstrated that exposure of wounds with0.9 kV/m to 1.9 kV/m chopped direct current (DC) electric field with a 30 micros repetition time favourably affected collagen synthesis and subsequent wound recovery.…”

Section: Role Of Microenvironment In Skin Regenerationmentioning

confidence: 99%

Skin bioprinting: a novel approach for creating artificial skin from synthetic and natural building blocks

2018

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Significant progress has been made over the past few decades in the development of in vitro-engineered substitutes that mimic human skin, either as grafts for the replacement of lost skin, or for the establishment of in vitro human skin models. Tissue engineering has been developing as a novel strategy by employing the recent advances in various fields such as polymer engineering, bioengineering, stem cell research and nanomedicine. Recently, an advancement of 3D printing technology referred as bioprinting was exploited to make cell loaded scaffolds to produce constructs which are more matching with the native tissue. Bioprinting facilitates the simultaneous and highly specific deposition of multiple types of skin cells and biomaterials, a process that is lacking in conventional skin tissue-engineering approaches. Bioprinted skin substitutes or equivalents containing dermal and epidermal components offer a promising approach in skin bioengineering. Various materials including synthetic and natural biopolymers and cells with or without signalling molecules like growth factors are being utilized to produce functional skin constructs. This technology emerging as a novel strategy to overcome the current bottle-necks in skin tissue engineering such as poor vascularization, absence of hair follicles and sweat glands in the construct.

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Effects of a specially pulsed electric field on an animal model of wound healing

Cited by 20 publications

References 17 publications

Electrical Stimulation Promotes Wound Healing by Enhancing Dermal Fibroblast Activity and Promoting Myofibroblast Transdifferentiation

Electrical Stimulation Promotes Wound Healing by Enhancing Dermal Fibroblast Activity and Promoting Myofibroblast Transdifferentiation

A Disposable Photovoltaic Patch Controlling Cellular Microenvironment for Wound Healing

Skin bioprinting: a novel approach for creating artificial skin from synthetic and natural building blocks

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