Effects of a Supersaturated Pulpal Fluid on the Formation of Caries-Like Lesions on the Roots of Human Teeth

Shellis, R.P.

doi:10.1159/000261614

Cited by 34 publications

(29 citation statements)

References 6 publications

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“…It has to be taken into consideration that the in vitro lesions are unable to simulate biological events such as bacterial penetration, collagen degradation, tubular occlusion and reactionary dentine [Shellis, 1994;Marquezan et al, 2009]. Also, a point of interest is whether bovine dentine is an appropriate substitute for human dentine.…”

Section: Discussionmentioning

confidence: 99%

Different Protocols to Produce Artificial Dentine Carious Lesions in vitro and in situ: Hardness and Mineral Content Correlation

et al. 2012

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This study compared dentine demineralization induced by in vitro and in situ models, and correlated dentine surface hardness (SH), cross-sectional hardness (CSH) and mineral content by transverse microradiography (TMR). Bovine dentine specimens (n = 15/group) were demineralized in vitro with the following: MC gel (6% carboxymethylcellulose gel and 0.1 m lactic acid, pH 5.0, 14 days); buffer I (0.05 m acetic acid solution with calcium, phosphate and fluoride, pH 4.5, 7 days); buffer II (0.05 m acetic acid solution with calcium and phosphate, pH 5.0, 7 days), and TEMDP (0.05 m lactic acid with calcium, phosphate and tetraethyl methyl diphosphonate, pH 5.0, 7 days). In an in situ study, 11 volunteers wore palatal appliances containing 2 bovine dentine specimens, protected with a plastic mesh to allow biofilm development. The volunteers dripped a 20% sucrose solution on each specimen 4 times a day for 14 days. In vitro and in situ lesions were analyzed using TMR and statistically compared by ANOVA. TMR and CSH/SH were submitted to regression and correlation analysis (p < 0.05). The in situ model produced a deep lesion with a high R value, but with a thin surface layer. Regarding the in vitro models, MC gel produced only a shallow lesion, while buffers I and II as well as TEMDP induced a pronounced subsurface lesion with deep demineralization. The relationship between CSH and TMR was weak and not linear. The artificial dentine carious lesions induced by the different models differed significantly, which in turn might influence further de- and remineralization processes. Hardness analysis should not be interpreted with respect to dentine mineral loss.

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Section: Discussionmentioning

confidence: 99%

Different Protocols to Produce Artificial Dentine Carious Lesions in vitro and in situ: Hardness and Mineral Content Correlation

et al. 2012

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“…These teeth were extracted only for clinically appropriate reasons using a protocol approved by the Institutional Review Board of the University of Iowa (IRB Approval #200010082). The extracted teeth were stored at 4°C in a phosphate buffered saline (PBS) for no more than 1 month, with a calcium phosphate saturation ratio of 11.52 for hydroxyapatite 29 .…”

Section: Teethmentioning

confidence: 99%

Effects of polar solvents and adhesive resin on the denaturation temperatures of demineralised dentine matrices

Armstrong

Jessop

Winn

et al. 2008

Journal of Dentistry

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SUMMARYObjectives-To measure the denaturation temperature (Td) of demineralised dentine matrix as a function of infiltration with water vs polar solvents vs adhesive resins.Methods-Small disks of normal dentine were completely demineralised in 0.5 M EDTA. Dried demineralised specimens were placed in water, methanol, ethanol, acetone, η-butanol or HEMA. Additional specimens were infiltrated with Prime & Bond NT and polymerised. All specimens sealed in high pressure pans and scanned using differential scanning calorimetry (DSC).Results-Demineralised dentine saturated with water showed a Td of 65.6°C that increased with saturation by methanol, ethanol, acetone, η-butanol or HEMA to 148.5°C. These increases in Td were inversely related to the molar concentration of the solvents and to their Hoy's solubility parameter for hydrogen bonding (δh, p<0.01), as well as directly related to the cube root of their molecular weights (p<0.001). The presence of adhesive resins also increased the Td of demineralised matrices to even higher values depending if the resin bonded dentine was measured after 24 h of water storage (166.8°C) or dry (172.7°C) storage.Conclusions-Solvents and monomers with low δh values (i.e. 100% HEMA) increase the Td of demineralized dentin above that produced by solvents with higher δh values such as methanol and water.

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“…If this hypothesis is correct, then the continual removal by enzymic and mechanical processes in vivo -not modelled in this study -could considerably enhance the effect of inhibitors, by improving access to the deeper parts of the lesion. In any case, it is recommended that magnesium ions should be included in undersaturated buffers for studying root-surface lesion formation, since re-precipitation of magnesium-containing calcium phosphates could influence lesion formation [Daculsi et al, 1987;Shellis, 1994].…”

Section: Discussionmentioning

confidence: 99%

“…In most lesions, which showed no sign of shrinkage, the outer lesion surface was defined by the root surface. Some of the more advanced lesions showed surface shrinkage and in measuring these lesions the plain axis of the crosswire was placed at the intact surfaces bordering the lesion and measurements taken from this line [Shellis, 1994]. For each lesion, 3-5 measurements were made in the middle third and a mean calculated.…”

Section: Evaluation Of Lesionsmentioning

confidence: 99%

Formation of Caries-Like Lesions in vitro on the Root Surfaces of Human Teeth in Solutions Simulating Plaque Fluid

Shellis¹

2010

Caries Res

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Lesion formation on root surfaces of human posterior teeth was studied in acetate/lactate buffers with a background electrolyte composition based on plaque fluid analyses. Lesion depth after 28 days at 37°C was measured in relation to: the presence or absence of cementum; the concentration of undissociated buffer; the presence or absence of magnesium ions at plaque fluid concentration. Each factor was evaluated at several values of –log(ion activity product for hydroxyapatite): pI_HA. Solutions were formulated to minimize variation in pH, which varied by ≤0.03 for a given comparison (individual pI_HA) and by 0.42–0.82 over the range of pI_HA within experiments. Lesions on surfaces from which cementum had been ground were significantly deeper than on intact surfaces, but this is considered to be due to subsurface mechanical damage and not to a solubility difference. Neither the concentration of undissociated buffer nor the presence of magnesium ions significantly affected lesion depth. Lesion depth was strongly influenced by the correlated variations in pI_HA and pH. At pI_HA 54 and 55, only extremely shallow lesions formed. From pI_HA 56, lesion depth increased with increasing pI_HA. The results confirm that the solubility of the mineral of root tissues is higher than that of hydroxyapatite, but indicate that it is probably lower than suggested by Hoppenbrouwers et al. [Arch Oral Biol 1987;32:319–322]. For calcium concentrations of 3–12 mM, the critical pH for root tissue mineral was calculated as 5.22–5.66 assuming solubility equivalent to pI_HA 54 and 5.08–5.51 assuming pI_HA 55.

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Effects of a Supersaturated Pulpal Fluid on the Formation of Caries-Like Lesions on the Roots of Human Teeth

Cited by 34 publications

References 6 publications

Different Protocols to Produce Artificial Dentine Carious Lesions in vitro and in situ: Hardness and Mineral Content Correlation

Different Protocols to Produce Artificial Dentine Carious Lesions in vitro and in situ: Hardness and Mineral Content Correlation

Effects of polar solvents and adhesive resin on the denaturation temperatures of demineralised dentine matrices

Formation of Caries-Like Lesions in vitro on the Root Surfaces of Human Teeth in Solutions Simulating Plaque Fluid

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