Effects of a synbiotic combination of Bacillus subtilis and yeast cell wall-derived glucomannan on cecal colonization of Salmonella Enteritidis in layer chickens

Effect of a Bacillus subtilis plus Yeast Cell Wall Synbiotic on Salmonella Enteritidis Colonization in Ceca of Layer Pullets

“…The prevalence results agree with findings by other researchers who reported lack of a significant effect of the synbiotic used on SE prevalence in 8-or 12-week-old pullets [36], 17-week-old pullets [28], and 60-week-old laying hens [23], respectively. However, in a previous study [30], BPQ11 significantly reduced SE prevalence in ceca by 50% in pullets challenged with SE at 3 weeks of age. In addition to prevalence, Salmonella contamination data also focus on number and serotype [45].…”

“…Price et al reported that the number of SE in the ceca at 7 DPC was numerically reduced (p = 0.14) by 0.78 log 10 MPN (most probable number)/g compared to the control when 60-week-old Hy-Line W36 fed synbiotic were inoculated with 7 × 10 7 CFU SE per hen [23]. In a previous study with BPQ11, no significant differences in cecal SE counts at 7, 10, or 13 DPC were observed between the control and BPQ11-fed Hy-Line W36 pullets challenged with an SE dose of 1.6 × 10 9 log 10 CFU per bird at 3 weeks of age [30].…”

“…In addition to the ileum and cloaca, cecal pouches are the preferred organs for SE colonization and establishment after it is ingested by chickens [35]. After an SE challenge, SE colonized the ceca of young layer pullets [21,26,29,30,36], layer pullets near sexual maturity [24,25,27,28], and adult laying hens [21][22][23]. In this study, orally infecting layer pullets with an SE dose of 6.3 × 10 7 CFU at 8 days of age resulted in colonization of the ceca, as evidenced by load and prevalence of SE in the ceca.…”

“…Work by Shaji and coworkers noted that SE dose, bird age and genetic strain, as well as Salmonella serotype, can be interacting and influencing factors and may explain differences in SE mitigation studies [43]. The various results from the studies discussed above [23,29,30] and those from the study reported here, all of which evaluated synbiotics consisting of a Bacillus probiotic plus yeast cell wall components, suggest that a more standardized Salmonella challenge model in layer pullets to evaluate similar synbiotics would be of interest.…”

“…In addition, Bacillus-based probiotics and yeast cell wall-based prebiotics are among the most used feed additives in poultry. Only recently, however, has there been published research, though limited, testing the effects of combinations of Bacillus and MOS on SE colonization in layer birds [23,[28][29][30]. In these publications, the synbiotics tested contained different Bacillus probiotics and yeast cell wall prebiotics and used either young pullets or adult hens of either Hy-Line or Dekalb genetics background.…”