Effects of a trichothecene, T-2 toxin, on proliferation and steroid production by porcine granulosa cells

Caloni, F.; Ranzenigo, G.; Crémonesi, F.; Spicer, L. J.

doi:10.1016/j.toxicon.2009.05.002

Cited by 53 publications

(29 citation statements)

References 47 publications

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“…Ndossi et al [26] in their study with H295R cells found no significant change in progesterone production of the cells at lower T-2 toxin concentrations (0.0005-0.05 ng mL -1 ) but the level increased significantly in these cells exposed to 5 ng mL -1 . Caloni et al [23] reported that 30 and 300 ng mL -1 of T-2 toxin completely inhibited FSH Fig. 2.…”

Section: Discussionmentioning

confidence: 96%

“…[12] T-2 and HT-2 toxin at lower dose 100 ng mL -1 in combination with IGF-I depending on the dose were able to inhibit progesterone release by porcine ovarian granulosa cells. [12] Likewise, in another in vitro study Caloni et al [23] (2009) reported that progesterone production was less sensitive to the inhibitory effects of T2 with 0.3 ng mL -1 having no effect and 1 ng mL -1 inhibiting progesterone production by only 30%. At 30 and 300 ng mL -1 , T2 completely inhibited FSH plus IGF-Iinduced progesterone production.…”

Section: Discussionmentioning

confidence: 97%

“…[12,[22][23][24][25] The influence of trichothecene mycotoxins such as T2 toxin and HT-2 toxin [12] and deoxynivalenol (DON) [22,24,25] on porcine ovarian granulosa cells focused on the processes of steroidogenesis was described in the previous studies. There is little data on the effect of T-2 toxin and HT-2 toxin and their combinations with growth factor IGF-I and metabolic hormones leptin and ghrelin on secretion activity of rabbit ovarian cells.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of T-2 toxin effect and its metabolite HT-2 toxin combined with insulin-like growth factor I, leptin and ghrelin on progesterone secretion by rabbit ovarian fragments

Maruniaková

Kádasi

Sirotkin

et al. 2015

Journal of Environmental Science and Health, Part B

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Assessment of A-trichothecene mycotoxins (T-2 and HT-2 toxins) effect combined with growth factor IGF-I, and the metabolic hormones leptin and ghrelin on progesterone secretion by rabbit ovarian fragments was studied. Rabbit ovarian fragments were incubated without (control group) or with T-2/HT-2 toxin, or their combinations with insulin-like growth factor I (IGF-I), leptin or ghrelin at various concentrations for 24 h. Secretion of progesterone was determined by ELISA. First, T-2 toxin and HT-2 toxins at all doses used (0.01, 0.1, 1, 10, and 100 ng mL(-1)) were not shown to be potential regulators of progesterone secretion in rabbit ovarian fragments. Second, T-2 toxin but not HT-2 toxin combined with IGF-I was shown to be potential regulator of progesterone secretion in rabbit ovarian fragments. T-2 toxin at all doses used (0.01; 0.1; 1; 10; and 100 ng mL(-1)) combined with IGF-I (at dose 100 ng mL(-1)) significantly (P < 0.05) decreased progesterone secretion by rabbit ovarian fragments. Third, T-2 toxin and HT-2 toxin at all doses used in the study (0.01, 0.1, 1, 10, and 100 ng mL(-1)) combined with leptin (at dose 1000 ng mL(-1)) were not shown to be potential regulators of progesterone secretion in rabbit ovarian fragments. Furthermore, T-2 toxin and HT-2 toxin at all doses used in the study (0.01, 0.1, 1, 10, and 100 ng mL(-1)) combined with ghrelin (500 ng mL(-1)) were not shown to be potential regulators of progesterone secretion in rabbit ovarian fragments. Results in this study showed that trichothecene as T-2 toxin combined with IGF-I but not HT-2 toxin was able to decrease progesterone secretion in rabbit ovarian fragments in vitro. Experimental results of T-2 and HT-2 toxins combined with leptin and ghrelin did not confirm ability to modulate progesterone secretion by ovarian fragments in rabbits.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of T-2 toxin effect and its metabolite HT-2 toxin combined with insulin-like growth factor I, leptin and ghrelin on progesterone secretion by rabbit ovarian fragments

Maruniaková

Kádasi

Sirotkin

et al. 2015

Journal of Environmental Science and Health, Part B

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“…[2] It is known that T-2 toxin had direct and potent dose-dependent effects on porcine granulosa cell steroidogenesis and proliferation. [27,29] Natural antioxidants as quercetin or resveratrol can modulate ovarian function, interfering with porcine granulosa cell steroidogenic activity. [12,28] Activity of SOD and GPx were significantly decreased by T-2 toxin in the rat ovarian granulosa cells.…”

Section: Discussionmentioning

confidence: 99%

Changes in antioxidant status of porcine ovarian granulosa cells after quercetin and T-2 toxin treatment

Capcarová

Petruška

Zbyňovská

et al. 2015

Journal of Environmental Science and Health, Part B

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The aim of this study was to determine the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), production of reactive oxygen species (ROS), and total antioxidant status (TAS) in porcine ovarian granulosa cells after quercetin and T-2 toxin exposure in vitro. Porcine ovarian granulosa cells were incubated with quercetin and T-2 toxin separately or in mutual combination at the doses of 1 ng/mL, 10 ng/mL, 100 ng/mL, and the control group without any additions for 24 h. In this study T-2 toxin developed stress reaction in porcine ovarian granulosa cells and increased generation of ROS. Quercetin had no effect in elimination of ROS generation induced by T-2 toxin, but was effective in maintaining and increasing of TAS, activities of SOD and GPx in porcine granulosa cells in vitro. These results contribute towards the understanding of cellular stress and its response.

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“…These abnormalities include retarded ovulation and decreased progesterone production (Huszenicza et al, 2000), disrupted corpus luteum maturation (Ványi et al, 1995), inhibited granulosa cell proliferation (Caloni et al, 2009), as well as reduced sperm motility and testosterone production (Kovács et al, 2011).…”

mentioning

confidence: 99%

T-2 mycotoxin slows down the development of mouse blastocysts, decreases their blastomere number and increases chromatin damage

Somoskői

Kovács

Cseh

2016

Acta Veterinaria Hungarica

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The mycotoxin T-2 has many harmful effects on mammalian cells and reproductive functions. In the present study, the in vitro effect of T-2 toxin on mouse blastocysts was examined. Embryos were cultured in media supplemented with 0.5, 0.75 and 1 ng/ml T-2. Different exposure times were applied [96 h (treatment I) or 24 h following 72 h in toxin-free media (treatment II)]. Blastomere number, nuclear chromatin status and blastocoel formation were investigated in blastocysts. Our data show that the effect of T-2 toxin may vary depending on the stage of the embryo at the start of exposure. At 96 h of exposure, the blastocysts had blastomeres with normal chromatin quality but their developmental potential was decreased. After 24 h of exposure applied following a 72-h culture, blastomeres had a higher level of chromatin damage, although their developmental potential was the same as in the control embryos. In both cases, decreased mitotic rate was found, which resulted in decreased blastomere number even at low toxin concentration.

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Effects of a trichothecene, T-2 toxin, on proliferation and steroid production by porcine granulosa cells

Cited by 53 publications

References 47 publications

Assessment of T-2 toxin effect and its metabolite HT-2 toxin combined with insulin-like growth factor I, leptin and ghrelin on progesterone secretion by rabbit ovarian fragments

Assessment of T-2 toxin effect and its metabolite HT-2 toxin combined with insulin-like growth factor I, leptin and ghrelin on progesterone secretion by rabbit ovarian fragments

Changes in antioxidant status of porcine ovarian granulosa cells after quercetin and T-2 toxin treatment

T-2 mycotoxin slows down the development of mouse blastocysts, decreases their blastomere number and increases chromatin damage

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