Effects of Abeliophyllum distichum Nakai Flower Extracts on Antioxidative Activities and Inhibition of DNA Damage

Abstract

-The present study attempts to evaluate antioxidant activities of extracts from Abeliophyllum distichum. Nakai flower. The samples were collected in Jangyyeon-myeon, Goesan-gun, Korea and extracted with either hot-water or ethyl acetate (EtOAC). In DPPH, hydroxyl radical scavenging activity and Fe 2+ chelating activity of EtOAC extracts were 93.41%, 98.43%, and 7.38%, while those of hot-water extracts were 86.93%, 41.33% and 47.68% at 200 μg/ml, respectively. In φX-174 RF I plasmid DNA cleavage assay, the protective effects of EtOAC and hot-water extracts against oxidative DNA damage were 82% and 17% at 200 μg/ml, respectively. Both extracts showed the protective effect of DNA migration by oxidative stress in intracellular DNA migration assay. Both extracts had no cytotoxity in NIH3T3 cells. Several polyphenolic compounds were identified such as 2-methoxy-benzoic acid, vanillic acid, phytol and pulegone by GC/MS. These results indicated that extracts of Abeliophyllum distichum Nakai flower showed antioxidant activities and protective activities against oxidative DNA damage and showed the possibility to be used as an effective natural antioxidants.

Protective and inhibitory effects of acteoside from Abeliophyllum distichum Nakai against oxidative DNA damage

“…Additionally, the effects of the antioxidant properties of AAD on oxidative DNA damage were also examined. Our previous studies suggested that A. distichum had potential antioxidant activities ( 49 , 50 ). DPPH and ABTS radicals are the most common and most stable chromogens used to estimate antioxidant activity of biological materials.…”

Anti-inflammatory effects of isoacteoside fromAbeliophyllum distichum

“…Phytochemical studies have identified two phenylethanol glycosides: verboscoside (acteoside) and arenarioside; two benzoquinolethanoids: cornoside and halleridone; and a lignin glucoside, epipinoresinol 4 0 -O-glucoside 3,4 . Some of these compounds have been reported as having antioxidant, anti-angiotensin and antidiabetic properties 2,5 . However, the compounds responsible for its anti-inflammatory actions remain elusive.…”

Antioxidant, Anti-inflammatory and Cytotoxicity on Human Lung Epithelial A549 Cells of Jerusalem artichoke (Helianthus tuberosus L.) Tuber

“…Phytonutrients used for food, cosmetic and pharmaceutical products not only have negligible amount of poison to our body, but also have strong biological activity (Ahn and Park, 2013). Most free radicals, being known as reactive oxygen species (ROS) and reactive nitrogen species (RNS) are considered to be a fundamental cause of physiological diseases such as cancer, aging, diabetes mellitus, Alzheimer's disease and coronary heart ailment (Bulkley, 1983;Cheng et al, 2003;Finkel and Holbrook, 2000).…”