Effects of Abscisic Acid Metabolites and Analogs on Freezing Tolerance and Gene Expression in Bromegrass (Bromus inermis Leyss) Cell Cultures

Robertson, A.J.; Reaney, Martin J. T.; Wilen, Ronald W.; Lamb, Nancy; Abrams, Suzanne R.; Gusta, L. V.

doi:10.1104/pp.105.3.823

Cited by 39 publications

(32 citation statements)

References 20 publications

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“…Total RNA was isolated from crown tissue of wheat using a modified hot-phenol method (Robertson et al, 1994). After isolation, total RNA (30 g per lane) was separated by electrophoresis on formaldehyde gels.…”

Section: Northern Analysismentioning

confidence: 99%

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Isolation, Chromosomal Localization, and Differential Expression of Mitochondrial Manganese Superoxide Dismutase and Chloroplastic Copper/Zinc Superoxide Dismutase Genes in Wheat1

et al. 1999

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Superoxide dismutase (SOD) gene expression was investigated to elucidate its role in drought and freezing tolerance in spring and winter wheat (Triticum aestivum). cDNAs encoding chloroplastic Cu/ZnSODs and mitochondrial MnSODs were isolated from wheat. MnSOD and Cu/ZnSOD genes were mapped to the long arms of the homologous group-2 and -7 chromosomes, respectively. Northern blots indicated that MnSOD genes were drought inducible and decreased after rehydration. In contrast, Cu/ZnSOD mRNA was not drought inducible but increased after rehydration. In both spring and winter wheat seedlings exposed to 2°C, MnSOD transcripts attained maximum levels between 7 and 49 d. Transcripts of Cu/ ZnSOD mRNA were detected sooner in winter than in spring wheat; however, they disappeared after 21 d of acclimation. Transcripts of both classes of SOD genes increased during natural acclimation in both spring and winter types. Exposure of fully hardened plants to three nonlethal freeze-thaw cycles resulted in Cu/Zn mRNA accumulation; however, MnSOD mRNA levels declined in spring wheat but remained unchanged in winter wheat. The results of the dehydration and freeze-thaw-cycle experiments suggest that winter wheat has evolved a more effective stress-repair mechanism than spring wheat.Active oxygen species such as superoxide, H 2 O 2 , and hydroxyl radicals are by-products of normal cell metabolism. These active oxygen species result in the peroxidation of membrane lipids (Mead, 1976), breakage of DNA strands (Brawn and Fridovich, 1981), and inactivation of enzymes (Fucci et al., 1983). The conditions leading to damage caused by active oxygen species are referred to as oxidative stress. Both chloroplasts and mitochondria can produce active oxygen species either under normal growth conditions or during exposure to various stresses. The PSI electron-transport chain contains a number of autooxidizable enzymes that reduce O 2 to superoxide (Badger, 1985; Asada and Takahashi, 1987; Asada, 1994), and evidence indicates that superoxide and H 2 O 2 can also be produced by PSII under high-light intensities (Landgraf et al., 1995).During mitochondrial respiration, reactive oxygen species are also generated via the reactions of the electrontransport chain (Rich and Bonner, 1978;Bowler et al., 1991).Active oxygen species are also generated during chemical and environmental stresses, including chilling and freezing (Wise and Naylor, 1987;Senaratna et al., 1988; Kendall and McKersie, 1989;Tsang et al., 1991;McKersie et al., 1993), drought (Perl-Treves and Galun, 1991; Price and Hendry, 1991), desiccation (Senaratna et al., 1985;Leprince et al., 1990), flooding (Hunter et al., 1983;Van Toai and Bolles, 1991), herbicide treatment (Malan et al., 1990; Kurepa et al., 1997), pathogen attack (Montalbini and Buonaurio, 1986; Buonaurio et al., 1987; Koch and Slusarenko, 1990), and ionizing radiation (Niwa et al., 1977).SODs are a group of metalloenzymes that protect cells from superoxide radicals by catalyzing the dismutation of the superoxide radical to...

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Section: Northern Analysismentioning

confidence: 99%

“…After isolation, total RNA (30 g per lane) was separated by electrophoresis on formaldehyde gels. The remainder of the RNA blotting and hybridization was as described previously (Robertson et al, 1994), except that 200 g of sonicated herring DNA was used instead of yeast tRNA.…”

Section: Northern Analysismentioning

confidence: 99%

Isolation, Chromosomal Localization, and Differential Expression of Mitochondrial Manganese Superoxide Dismutase and Chloroplastic Copper/Zinc Superoxide Dismutase Genes in Wheat1

et al. 1999

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“…Vol. 108, 1995 induction of synthesis of two ABA-inducible proteins in barley embryos and in blocking the GA,-induced accumulation of a-amylase in aleurone layers (Robertson et al, 1994). The latter observation was also made by Walton (1983) using natural PA extracted from plant tissues.…”

mentioning

confidence: 60%

“…On the other hand, natural PA has been shown to be ineffective in inducing ABA-specific proteins in aleurone layers (Lin and Ho, 1986) and only weakly active in inhibiting the growth of cultured maize cells (Balsevich et al, 1994a(Balsevich et al, , 1994b. Racemic PA was inactive in inducing freezing tolerance in bromegrass cultured cells (Robertson et al, 1994). (-)-7'-HydroxyABA was weakly active in inhibiting the growth of maize cells (Balsevich et al, 1994a(Balsevich et al, , 1994b.…”

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confidence: 99%

“…The biological activity of (+I-7'-hydroxyABA has not been reported, whereas the biological activity of 8'-hydroxyABA is not known because the compound spontaneously cyclizes to PA in solution. The ABA analog racemic 2',3'-dihydroABA has potent biological activity in the induction of freezing tolerance (Robertson et al, 1994) and in the reversal of GA,-induced a-amylase activity (Hill et al, 1992). Furthermore, the (+)-optical isomer was a germination inhibitor in wheat embryos (Walker-Simmons et al, 1992).…”

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Abscisic Acid Structure-Activity Relationships in Barley Aleurone Layers and Protoplasts (Biological Activity of Optically Active, Oxygenated Abscisic Acid Analogs)

Hill¹,

Liu²,

Durnin³

et al. 1995

Plant Physiol.

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Promotion of the albumin storage protein gene response had a more strict stereochemical requirement for elicitation of an ABA response than inhibition of a-amylase gene expression. l h e naturally occurring stereoisomer of ABA and its metabolites were more effective at followed by 7'-hydroxyABA, with phaseic acid being the least active. Racemic 8'-hydroxy-2',3'-dihydroABA, an analog of 8'-hydroxyABA, was inactive, whereas racemic 2',3'-dihydroABA was as effective as ABA. The differences in response of the same tissue to the ABA enantiomers lead us to conclude that there exists more than one type of ABA receptor and/or multiple signal transduction pathways in barley aleurone tissue.

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Exploring In‐built Defense Mechanisms in Plants under Heat Stress

Surabhi

Seth

2020

Heat Stress Tolerance in Plants

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Effects of Abscisic Acid Metabolites and Analogs on Freezing Tolerance and Gene Expression in Bromegrass (Bromus inermis Leyss) Cell Cultures

Cited by 39 publications

References 20 publications

Isolation, Chromosomal Localization, and Differential Expression of Mitochondrial Manganese Superoxide Dismutase and Chloroplastic Copper/Zinc Superoxide Dismutase Genes in Wheat1

Isolation, Chromosomal Localization, and Differential Expression of Mitochondrial Manganese Superoxide Dismutase and Chloroplastic Copper/Zinc Superoxide Dismutase Genes in Wheat1

Abscisic Acid Structure-Activity Relationships in Barley Aleurone Layers and Protoplasts (Biological Activity of Optically Active, Oxygenated Abscisic Acid Analogs)

Exploring In‐built Defense Mechanisms in Plants under Heat Stress

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