Effects of AC‐Electrolysis on the Enzymatic Activity of Glucose Oxidase

Abstract

The change in the activity of glucose oxidase subjected to an asymmetrical alternating current (AC) electric field is investigated via horseradish peroxidase (HRP)-coupled bioassay. The effect of the amplitude, frequency and enzyme concentration have been shown to affect the enzyme activity towards glucose oxidation. The decrease in the enzyme activity is directly related to the change in pH and temperature of the GOx solution during AC electrolysis. The enzyme activity reduces with increasing amplitude, enzyme concentration and decreasing frequency. Results from UV-vis, FT-IR and UV CD spectroscopy showed that the AC treated GOx samples undergo structural modifications.

Enhanced electrochromic switching speed and electrochemical stability of conducting polymer film on an ionic liquid functionalized ITO electrode

“…The smaller and more uniform structure may lead to a larger contact area and more channels, which could facilitate counterion diffusion and improve the switching speed. 30,31 The peak at 1068 cm À1 corresponds to the asymmetric stretching vibrations of B-F. 30 The spectrum of the PTBTPA-[BMIM]BF 4 film contains all the characteristic bands of above two compounds with a little difference (maybe ascribed to the interaction between the two compounds). 1b In the spectrum of the pure PTBTPA film, the peak located at 1597 cm À1 should be assigned to the C-C stretching vibrations in the phenyl ring.…”

An electronic approach to minimising moisture-associated skin damage in ostomy patients

“…2) which can actively measure the pH and respond accordingly. The electrochemical control of processes that involve either the production of protons (H + ) or their consumption (and hence enable pH manipulation) have been successfully demonstrated in a number of electroanalytical [29,30] and electroporation systems [31][32][33][34] but the underpinning reactions are now being extensively researched in solar conversion and hydrogen fuel cell technologies [35][36][37][38][39][40][41]. Our contention is that these processes which, in principle, can lead to changes in pH profile at the electrode, could be harnessed to control the pH at the skin surface.…”

Alternating‐Current Electrolysis for the Production of Phenol from Benzene

“…The advantage of AC electrolysis over DC electrolysis has been reported for the purification of organic pollutants [16,17] and for the synthesis of chemicals. [18,19] Based on these observations and assumptions, it is expected that the electrochemical synthesis of phenol could be improved by optimizing the AC frequency for Reactions (1), (2), (3), and (4). The goals of the present work are to: 1) characterize both the anode and cathode reactions under DC with respect to the current efficiency for phenol production and the selectivity toward phenol; 2) clarify the reaction mechanism by various means, including electrochemical, kinetic, and spectroscopic techniques; and 3) enhance the phenol production using AC with optimal frequency.…”