Effects of acetic acid, furfural and catechol combinations on ethanol fermentation of Kluyveromyces marxianus

Oliva, J.; Negro, M.J.; Saéz, Felicia; Ballesteros, Ignacio; Manzanares, Paloma; González, Alberto; Ballesteros, Mercedes

doi:10.1016/j.procbio.2005.12.003

Cited by 65 publications

(49 citation statements)

References 26 publications

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“…The latter only depended on laccase dosage. Previous works showed a similar reduction of total phenols close to 80% in presence of higher laccase of 10 IU/g DW of substrate, even though initial phenol concentration was lower (2.5 g/L versus 4 g/L) [4]. Herein, the saturation of phenol removal was achieved from 2.75 IU/g DW laccase dosage.…”

Section: Presaccharification and Laccase Detoxification Stepsupporting

confidence: 63%

“…Concentration of aromatic phenols, such as vanillin, in the range of 0.9-1.2 g/L caused oxidative stress and mitochondrial fragmentation in Saccharomyces cerevisiae [25]. Although this range of concentrations did not inhibit the growth of S. cerevisiae [26], the combination of different degradation products, inhibited the growth of K. marxianus [4]. Phenolic compounds act cumulatively.…”

Section: Steam Explosion Pretreatmentmentioning

confidence: 96%

“…For inoculation, active cultures were obtained in 250 mL flasks with 100 mL of growth medium containing 20 g/L glucose, 5 g/L yeast extract, 2 g/L NH 4 Cl, 1 g/L KH 2 PO 4 , and 0.3 g/L MgSO 4 ‧ 7H 2 O. After 16h on a rotary shaker at 180 rpm at 42°C, the cultures were centrifuged at 5000 rpm for 5 min.…”

Section: Microorganisms and Mediamentioning

confidence: 99%

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Optimization of the laccase detoxification step in hybrid hydrolysis and fermentation processes from wheat straw by K. marxianus CECT 10875

Oliva-Taravilla¹,

Tomás‐Pejó²,

Demuez³

et al. 2016

Bioethanol

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The addition of laccase enzymes reduces the amount of phenols present in lignocellulosic pretreated materials and increases their fermentability. However, laccase addition in combination with cellulases reduces hydrolysis yields. In this work, hybrid hydrolysis and fermentation (HHF) configuration allowed overcoming the negative effect of laccase treatment on enzymatic hydrolysis. Furthermore, the effects of different laccase dosages, length of detoxification time and inoculum size on ethanol production were evaluated. In the evaluated configurations, the different laccase dosages did not show any significant effect on enzymatic hydrolysis. The lowest laccase dosage (0.5 IU/g DW) removed ~70% of total phenols which was enough to reach the highest ethanol production yields (~10 g/L) using K. marxianus CECT 10875. Shorter detoxification times and larger inoculum sizes had a positive impact on both ethanol production and volumetric productivity. These optimal detoxification conditions enable the fermentation of inhibitory slurries by reducing the overall time and cost of the process.

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Section: Presaccharification and Laccase Detoxification Stepsupporting

confidence: 63%

Section: Steam Explosion Pretreatmentmentioning

confidence: 96%

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Optimization of the laccase detoxification step in hybrid hydrolysis and fermentation processes from wheat straw by K. marxianus CECT 10875

Oliva-Taravilla¹,

Tomás‐Pejó²,

Demuez³

et al. 2016

Bioethanol

Self Cite

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“…This means that part of the sugars released from the polymer is degraded before the end of the reaction. In addition, the chemical hydrolysis usually produces small amounts of side products that can inhibit the subsequent fermentation step (Oliva et al 2006). Enzymatic hydrolysis is an ideal approach for converting lignocellulose into sugars because mild reaction conditions can be used resulting in negligible by-products at high sugar yields.…”

Section: Enzymatic Hydrolysis Of Lignocellulosic Materialsmentioning

confidence: 99%

Topochemistry, Porosity and Chemical Composition Affecting Enzymatic Hydrolysis of Lignocellulosic Materials

Milagres

Carvalho²,

Ferraz³

2010

Routes to Cellulosic Ethanol

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“…furfural and 5-hydroxymethylfurfural, respectively, from corresponding polysaccharides (Palmqvist and Hahn-Hagerdal 2000). The effects of furfural and 5-hydroxymethylfurfural have been studied, to some extent, in connection with biotechnological production of bioethanol or hydrogen from lignocellulose biomass (Oliva et al 2006;Lu et al 2007;Bellido et al 2011;Paulova et al 2012). Inhibition of at least three enzymes of the central carbon metabolism has been proven (Modig et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Quantitation of the Inhibition Effect of Model Compounds Representing Plant Biomass Degradation Products on Methane Production

et al. 2017

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During the steam explosion pretreatment of plant biomass, degradation products are generated, and some of these have inhibitory activity against biogas production. The aim of this study was to investigate and quantify the effect of selected model inhibitory compounds on methane production. The results showed no significant inhibition of methane production by furfural at concentrations below 1 g/L. In addition, the microbial community was able to restore biogas production inhibited by this compound after a certain time. 5-hydroxymethylfurfural was evaluated as a more potent inhibitor, with a significant effect above 0.2 g/L. Both compounds were more effective inhibitors with cellulose as the carbon substrate, probably reflecting higher sensitivity of the cellulolytic step in biogas production. No significant inhibition was observed for the phenolic compounds tested, gallic and tannic acids, at concentrations of up to 2 g/L. Thus, the compounds investigated should not represent a problem for the biogas production involving steam explosion preprocessed plant biomass.

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Effects of acetic acid, furfural and catechol combinations on ethanol fermentation of Kluyveromyces marxianus

Cited by 65 publications

References 26 publications

Optimization of the laccase detoxification step in hybrid hydrolysis and fermentation processes from wheat straw by K. marxianus CECT 10875

Optimization of the laccase detoxification step in hybrid hydrolysis and fermentation processes from wheat straw by K. marxianus CECT 10875

Topochemistry, Porosity and Chemical Composition Affecting Enzymatic Hydrolysis of Lignocellulosic Materials

Quantitation of the Inhibition Effect of Model Compounds Representing Plant Biomass Degradation Products on Methane Production

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