Effects of Acetone Fraction From Buchenavia tomentosa Aqueous Extract and Gallic Acid on Candida albicans Biofilms and Virulence Factors

Abstract

A promising anti-Candida activity of Buchenavia tomentosa extracts was recently described. In the present work, experiments were carried out to determine the fraction with higher antifungal activity from a B. tomentosa extract. Acetone fraction (AF) was obtained from the aqueous extract from dried leaves (5 min/100°C) and it was the most effective one. Gallic acid (GA) was identified by electrospray ionization mass spectrometry (ESI–MS) and also chosen to perform antifungal tests due to its promising activity on Candida albicans. Minimal inhibitory and fungicidal concentrations (MIC and MFC) were determined by broth microdilution technique. The effect on virulence factors of C. albicans was evaluated, and the cytotoxicity was determined. MIC50 and MIC90 values were both equal to 0.625 mg ml-1 for AF and 2.5 and 5 mg ml-1, respectively, for GA. AF and GA showed ability to inhibit C. albicans adherence and to disrupt 48 h-biofilm. AF and GA were effective in reducing the formation of hyphae of C. albicans SC5314. AF and GA decreased adherence of C. albicans to oral epithelial cells. AF and GA showed slight to moderate toxicity to Vero cells. This result suggests further studies for topic use of these compounds. AF, which contains a combination of several molecules, presented greater potential of antimicrobial activity than GA, with lower values of MIC and lower cytoxicity.

Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli-Induced Injury

“…Previous works reported the predominant presence of flavonoids and alkaloids in B. tetraphylla and other species from the same genus [20,21,43,44]. In this present research, the most bioactive fractions (BTFC and BTFD) showed a similar chemical composition with the presence of aliphatic (terpenes, fatty acids, carbohydrates) and aromatic compounds (phenolic compounds).…”

Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species

“…In this way, the extracts could be acting on extracellular matrix components, which are more present in mature biofilms. Similar results were obtained by Teodoro et al [22] when studying the effects of a Buchenavia tomentosa acetone fraction on Candida biofilms.…”

“…Alalwan et al [25] performed different experiments with curcumin and found that this substance interferes with the adhesion of C. albicans SC5314 to denture materials. Teodoro et al [22] demonstrated that an acetone fraction from a Buchenavia tomentosa extract had the ability to inhibit C. albicans ATCC 18804 and C. albicans SC 5314 adherence and to disrupt 48 h biofilm. However, they also verified that the acetone fraction and gallic acid showed slight to moderate toxicity to Vero cells.…”

Anadenanthera Colubrina vell Brenan: anti-Candida and antibiofilm activities, toxicity and therapeutical action

“…Our findings have shown an antibiofilm activity superior to that shown by other studies with natural products. 36,37 Although we used higher concentrations, we proved that they are well below the dose considered toxic in our model in vivo.…”